Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeS and yeeT are separated by 62 nucleotides yeeT and yeeU are separated by 73 nucleotides yeeU and yeeV are separated by 88 nucleotides yeeV and yeeW overlap by 4 nucleotides yeeW and yoeD are separated by 441 nucleotides yoeD and yoeF are separated by 68 nucleotides
b2002: yeeS - CP4-44 prophage; predicted DNA repair protein (NCBI), at 2,074,332 to 2,074,778
yeeS
b2003: yeeT - CP4-44 prophage; predicted protein (NCBI), at 2,074,841 to 2,075,062
yeeT
b2004: yeeU - CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI), at 2,075,136 to 2,075,504
yeeU
b2005: yeeV - CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system (NCBI), at 2,075,593 to 2,075,967
yeeV
b2006: yeeW - CP4-44 prophage; predicted protein (RefSeq), at 2,075,964 to 2,076,131
yeeW
b4642: yoeD - no description, at 2,076,573 to 2,076,701
yoeD
b4538: yoeF - hypothetical protein (RefSeq), at 2,076,770 to 2,076,955
yoeF
Position (kb)
2075
2076 Strain fitness (log2 ratio)
-1
0
1 at 2074.622 kb on + strand, within yeeS at 2074.649 kb on + strand, within yeeS at 2074.657 kb on - strand, within yeeS at 2074.690 kb on + strand, within yeeS at 2074.696 kb on + strand, within yeeS at 2074.696 kb on - strand, within yeeS at 2074.782 kb on + strand at 2074.865 kb on + strand, within yeeT at 2074.920 kb on - strand, within yeeT at 2074.949 kb on - strand, within yeeT at 2074.949 kb on - strand, within yeeT at 2075.051 kb on + strand at 2075.051 kb on + strand at 2075.051 kb on + strand at 2075.199 kb on - strand, within yeeU at 2075.199 kb on - strand, within yeeU at 2075.201 kb on + strand, within yeeU at 2075.209 kb on + strand, within yeeU at 2075.302 kb on + strand, within yeeU at 2075.407 kb on + strand, within yeeU at 2075.416 kb on + strand, within yeeU at 2075.452 kb on - strand, within yeeU at 2075.452 kb on - strand, within yeeU at 2075.642 kb on + strand, within yeeV at 2075.642 kb on + strand, within yeeV at 2075.827 kb on + strand, within yeeV at 2075.848 kb on - strand, within yeeV at 2075.848 kb on - strand, within yeeV at 2075.933 kb on + strand at 2076.005 kb on + strand, within yeeW at 2076.019 kb on + strand, within yeeW at 2076.019 kb on + strand, within yeeW at 2076.081 kb on + strand, within yeeW at 2076.111 kb on - strand, within yeeW at 2076.111 kb on - strand, within yeeW at 2076.134 kb on + strand at 2076.134 kb on + strand at 2076.161 kb on - strand at 2076.161 kb on - strand at 2076.178 kb on - strand at 2076.184 kb on - strand at 2076.251 kb on + strand at 2076.332 kb on - strand at 2076.415 kb on - strand at 2076.436 kb on + strand at 2076.436 kb on + strand at 2076.462 kb on + strand at 2076.462 kb on + strand at 2076.549 kb on - strand at 2076.611 kb on + strand, within yoeD at 2076.670 kb on - strand, within yoeD at 2076.697 kb on + strand at 2076.881 kb on - strand, within yoeF at 2076.886 kb on - strand, within yoeF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 2,074,622 + yeeS b2002 0.65 +0.1 2,074,649 + yeeS b2002 0.71 +0.4 2,074,657 - yeeS b2002 0.73 -0.3 2,074,690 + yeeS b2002 0.80 -0.6 2,074,696 + yeeS b2002 0.81 -0.5 2,074,696 - yeeS b2002 0.81 -0.3 2,074,782 + -0.1 2,074,865 + yeeT b2003 0.11 -1.0 2,074,920 - yeeT b2003 0.36 +0.2 2,074,949 - yeeT b2003 0.49 -0.0 2,074,949 - yeeT b2003 0.49 -0.2 2,075,051 + +0.5 2,075,051 + -0.7 2,075,051 + -0.8 2,075,199 - yeeU b2004 0.17 +0.4 2,075,199 - yeeU b2004 0.17 +0.1 2,075,201 + yeeU b2004 0.18 +0.2 2,075,209 + yeeU b2004 0.20 +0.2 2,075,302 + yeeU b2004 0.45 -0.8 2,075,407 + yeeU b2004 0.73 +0.3 2,075,416 + yeeU b2004 0.76 -0.1 2,075,452 - yeeU b2004 0.86 -0.3 2,075,452 - yeeU b2004 0.86 -1.0 2,075,642 + yeeV b2005 0.13 +0.2 2,075,642 + yeeV b2005 0.13 +0.7 2,075,827 + yeeV b2005 0.62 -0.2 2,075,848 - yeeV b2005 0.68 +0.0 2,075,848 - yeeV b2005 0.68 +0.5 2,075,933 + -0.4 2,076,005 + yeeW b2006 0.24 -0.0 2,076,019 + yeeW b2006 0.33 +0.2 2,076,019 + yeeW b2006 0.33 -0.7 2,076,081 + yeeW b2006 0.70 +0.1 2,076,111 - yeeW b2006 0.88 +0.3 2,076,111 - yeeW b2006 0.88 -0.9 2,076,134 + +0.2 2,076,134 + +0.2 2,076,161 - +0.4 2,076,161 - -0.3 2,076,178 - +0.1 2,076,184 - -0.5 2,076,251 + -0.0 2,076,332 - -0.7 2,076,415 - +0.1 2,076,436 + -0.0 2,076,436 + +0.7 2,076,462 + -0.5 2,076,462 + +0.2 2,076,549 - -0.2 2,076,611 + yoeD b4642 0.29 -0.0 2,076,670 - yoeD b4642 0.75 +0.7 2,076,697 + -0.4 2,076,881 - yoeF b4538 0.60 +0.4 2,076,886 - yoeF b4538 0.62 +0.2
Or see this region's nucleotide sequence