Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynhE and sufA are separated by 8 nucleotides sufA and rydB are separated by 326 nucleotides rydB and ydiH are separated by 153 nucleotides ydiH and ydiI are separated by 99 nucleotides ydiI and ydiJ overlap by 4 nucleotides
b1683: ynhE - orf, hypothetical protein (VIMSS), at 1,760,546 to 1,762,033
ynhE
b1684: sufA - iron-sulfur cluster assembly scaffold protein (NCBI), at 1,762,042 to 1,762,410
sufA
b4430: rydB - regulatory sRNA (NCBI), at 1,762,737 to 1,762,804
rydB
b1685: ydiH - predicted protein (RefSeq), at 1,762,958 to 1,763,146
ydiH
b1686: ydiI - hypothetical protein (NCBI), at 1,763,246 to 1,763,656
ydiI
b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709
ydiJ
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-1
0
1
2 at 1762.235 kb on - strand, within sufA at 1762.235 kb on - strand, within sufA at 1762.245 kb on - strand, within sufA at 1762.437 kb on + strand at 1762.521 kb on + strand at 1762.546 kb on - strand at 1762.891 kb on - strand at 1762.891 kb on - strand at 1762.904 kb on - strand at 1763.084 kb on - strand, within ydiH at 1763.150 kb on - strand at 1763.150 kb on - strand at 1763.186 kb on - strand at 1763.195 kb on + strand at 1763.195 kb on + strand at 1763.545 kb on + strand, within ydiI at 1763.577 kb on - strand, within ydiI at 1763.577 kb on - strand, within ydiI at 1763.692 kb on - strand at 1763.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 1,762,235 - sufA b1684 0.52 +0.2 1,762,235 - sufA b1684 0.52 -0.2 1,762,245 - sufA b1684 0.55 +0.1 1,762,437 + +2.2 1,762,521 + -0.4 1,762,546 - +0.7 1,762,891 - +0.1 1,762,891 - -1.2 1,762,904 - +0.3 1,763,084 - ydiH b1685 0.67 +0.4 1,763,150 - -0.1 1,763,150 - -0.7 1,763,186 - -1.2 1,763,195 + -0.1 1,763,195 + +0.5 1,763,545 + ydiI b1686 0.73 -0.0 1,763,577 - ydiI b1686 0.81 -0.2 1,763,577 - ydiI b1686 0.81 +0.0 1,763,692 - -0.0 1,763,886 - +0.2
Or see this region's nucleotide sequence