Strain Fitness in Escherichia coli BW25113 around b1362

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydaV and ydaW overlap by 29 nucleotidesydaW and rzpR are separated by 0 nucleotidesrzpR and rzoR overlap by 245 nucleotidesrzoR and trkG are separated by 196 nucleotides b1360: ydaV - Rac prophage; predicted DNA replication protein (NCBI), at 1,420,007 to 1,420,753 ydaV b1361: ydaW - orf, hypothetical protein (VIMSS), at 1,420,725 to 1,421,224 ydaW b1362: rzpR - putative Rac prophage endopeptidase (VIMSS), at 1,421,225 to 1,421,668 rzpR b4528: rzoR - Rac prophage; predicted lipoprotein (NCBI), at 1,421,424 to 1,421,609 rzoR b1363: trkG - Rac prophage; potassium transporter subunit (NCBI), at 1,421,806 to 1,423,263 trkG Position (kb) 1421 1422Strain fitness (log2 ratio) -2 -1 0 1 2at 1420.436 kb on - strand, within ydaVat 1420.589 kb on + strand, within ydaVat 1420.831 kb on - strand, within ydaWat 1421.276 kb on - strand, within rzpRat 1421.536 kb on + strandat 1421.536 kb on + strandat 1421.602 kb on + strandat 1421.612 kb on + strand, within rzpRat 1421.612 kb on + strand, within rzpRat 1421.623 kb on + strand, within rzpRat 1421.623 kb on + strand, within rzpRat 1421.638 kb on + strandat 1421.638 kb on + strandat 1421.641 kb on + strandat 1421.672 kb on + strandat 1421.672 kb on + strandat 1421.813 kb on + strandat 1421.838 kb on + strandat 1421.838 kb on + strandat 1421.841 kb on + strandat 1421.841 kb on + strandat 1421.846 kb on + strandat 1421.847 kb on + strandat 1421.848 kb on + strandat 1421.848 kb on + strandat 1421.859 kb on + strandat 1421.875 kb on + strandat 1421.891 kb on + strandat 1421.895 kb on + strandat 1421.983 kb on + strand, within trkGat 1422.251 kb on + strand, within trkGat 1422.251 kb on + strand, within trkGat 1422.308 kb on - strand, within trkGat 1422.353 kb on + strand, within trkGat 1422.475 kb on + strand, within trkG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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1,420,436 - ydaV b1360 0.57 +1.3
1,420,589 + ydaV b1360 0.78 -0.3
1,420,831 - ydaW b1361 0.21 -2.1
1,421,276 - rzpR b1362 0.11 +0.4
1,421,536 + -0.2
1,421,536 + +0.0
1,421,602 + -1.6
1,421,612 + rzpR b1362 0.87 +0.3
1,421,612 + rzpR b1362 0.87 +0.4
1,421,623 + rzpR b1362 0.90 +0.4
1,421,623 + rzpR b1362 0.90 +0.4
1,421,638 + +1.0
1,421,638 + -0.5
1,421,641 + +0.1
1,421,672 + +0.5
1,421,672 + +0.4
1,421,813 + -0.1
1,421,838 + +2.5
1,421,838 + +0.3
1,421,841 + +0.1
1,421,841 + +0.1
1,421,846 + +0.1
1,421,847 + +0.0
1,421,848 + -0.4
1,421,848 + +0.1
1,421,859 + +0.1
1,421,875 + -0.3
1,421,891 + +1.5
1,421,895 + -0.2
1,421,983 + trkG b1363 0.12 +1.5
1,422,251 + trkG b1363 0.31 +1.5
1,422,251 + trkG b1363 0.31 +1.5
1,422,308 - trkG b1363 0.34 +1.5
1,422,353 + trkG b1363 0.38 +1.5
1,422,475 + trkG b1363 0.46 -0.5

Or see this region's nucleotide sequence