Strain Fitness in Escherichia coli BW25113 around b1360

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydaT and ydaU are separated by 12 nucleotidesydaU and ydaV are separated by 6 nucleotidesydaV and ydaW overlap by 29 nucleotidesydaW and rzpR are separated by 0 nucleotidesrzpR and rzoR overlap by 245 nucleotides b1358: ydaT - Rac prophage; predicted protein (NCBI), at 1,418,708 to 1,419,130 ydaT b1359: ydaU - Rac prophage; conserved protein (NCBI), at 1,419,143 to 1,420,000 ydaU b1360: ydaV - Rac prophage; predicted DNA replication protein (NCBI), at 1,420,007 to 1,420,753 ydaV b1361: ydaW - orf, hypothetical protein (VIMSS), at 1,420,725 to 1,421,224 ydaW b1362: rzpR - putative Rac prophage endopeptidase (VIMSS), at 1,421,225 to 1,421,668 rzpR b4528: rzoR - Rac prophage; predicted lipoprotein (NCBI), at 1,421,424 to 1,421,609 rzoR Position (kb) 1420 1421Strain fitness (log2 ratio) -2 -1 0 1at 1419.186 kb on + strandat 1419.186 kb on + strandat 1419.235 kb on - strand, within ydaUat 1419.360 kb on - strand, within ydaUat 1419.393 kb on - strand, within ydaUat 1419.393 kb on - strand, within ydaUat 1419.797 kb on + strand, within ydaUat 1419.910 kb on + strand, within ydaUat 1419.933 kb on - strandat 1419.988 kb on - strandat 1420.068 kb on - strandat 1420.436 kb on - strand, within ydaVat 1420.589 kb on + strand, within ydaVat 1420.831 kb on - strand, within ydaWat 1421.276 kb on - strand, within rzpRat 1421.536 kb on + strandat 1421.536 kb on + strandat 1421.602 kb on + strandat 1421.612 kb on + strand, within rzpRat 1421.612 kb on + strand, within rzpRat 1421.623 kb on + strand, within rzpRat 1421.623 kb on + strand, within rzpRat 1421.638 kb on + strandat 1421.638 kb on + strandat 1421.641 kb on + strandat 1421.672 kb on + strandat 1421.672 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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1,419,186 + +0.2
1,419,186 + +0.8
1,419,235 - ydaU b1359 0.11 -0.2
1,419,360 - ydaU b1359 0.25 -0.3
1,419,393 - ydaU b1359 0.29 -0.1
1,419,393 - ydaU b1359 0.29 -0.5
1,419,797 + ydaU b1359 0.76 +0.2
1,419,910 + ydaU b1359 0.89 +0.1
1,419,933 - -0.0
1,419,988 - +0.1
1,420,068 - -0.5
1,420,436 - ydaV b1360 0.57 +1.3
1,420,589 + ydaV b1360 0.78 -0.3
1,420,831 - ydaW b1361 0.21 -2.1
1,421,276 - rzpR b1362 0.11 +0.4
1,421,536 + -0.2
1,421,536 + +0.0
1,421,602 + -1.6
1,421,612 + rzpR b1362 0.87 +0.3
1,421,612 + rzpR b1362 0.87 +0.4
1,421,623 + rzpR b1362 0.90 +0.4
1,421,623 + rzpR b1362 0.90 +0.4
1,421,638 + +1.0
1,421,638 + -0.5
1,421,641 + +0.1
1,421,672 + +0.5
1,421,672 + +0.4

Or see this region's nucleotide sequence