Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafX and ykfF are separated by 97 nucleotides ykfF and ykfB are separated by 76 nucleotides ykfB and yafY are separated by 22 nucleotides yafY and ykfL overlap by 1 nucleotides ykfL and ykfK are separated by 1 nucleotides ykfK and yafZ are separated by 216 nucleotides
b0248: yafX - CP4-6 prophage; predicted protein (NCBI), at 263,972 to 264,430
yafX
b0249: ykfF - CP4-6 prophage; predicted protein (NCBI), at 264,528 to 264,767
ykfF
b0250: ykfB - CP4-6 prophage; predicted protein (NCBI), at 264,844 to 265,311
ykfB
b0251: yafY - CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq), at 265,334 to 265,777
yafY
b4627: ykfL - no description, at 265,777 to 265,998
ykfL
b4628: ykfK - no description, at 266,000 to 266,191
ykfK
b0252: yafZ - orf, hypothetical protein (VIMSS), at 266,408 to 267,229
yafZ
Position (kb) 265
266 Strain fitness (log2 ratio) -1
0
1 at 264.426 kb on + strand at 264.483 kb on + strand at 264.539 kb on - strand at 264.539 kb on - strand at 264.542 kb on + strand at 264.550 kb on - strand at 264.554 kb on + strand, within ykfF at 264.674 kb on - strand, within ykfF at 264.701 kb on - strand, within ykfF at 264.752 kb on + strand at 264.752 kb on + strand at 264.953 kb on + strand, within ykfB at 265.019 kb on - strand, within ykfB at 265.140 kb on - strand, within ykfB at 265.234 kb on - strand, within ykfB at 265.234 kb on - strand, within ykfB at 265.275 kb on - strand at 265.362 kb on + strand at 265.426 kb on - strand, within yafY at 265.436 kb on - strand, within yafY at 265.519 kb on - strand, within yafY at 265.903 kb on + strand, within ykfL at 265.906 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.951 kb on - strand, within ykfL at 266.018 kb on + strand at 266.018 kb on + strand at 266.187 kb on + strand at 266.217 kb on - strand at 266.258 kb on + strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.353 kb on - strand at 266.433 kb on + strand at 266.436 kb on - strand at 266.449 kb on + strand at 266.464 kb on - strand at 266.569 kb on - strand, within yafZ at 266.668 kb on - strand, within yafZ at 266.773 kb on - strand, within yafZ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 264,426 + +0.2 264,483 + +0.1 264,539 - +0.2 264,539 - -0.2 264,542 + -0.4 264,550 - +0.2 264,554 + ykfF b0249 0.11 -0.2 264,674 - ykfF b0249 0.61 -0.5 264,701 - ykfF b0249 0.72 -0.0 264,752 + -0.1 264,752 + -0.4 264,953 + ykfB b0250 0.23 -0.0 265,019 - ykfB b0250 0.37 +0.2 265,140 - ykfB b0250 0.63 -0.6 265,234 - ykfB b0250 0.83 -0.4 265,234 - ykfB b0250 0.83 +0.1 265,275 - +0.0 265,362 + +0.1 265,426 - yafY b0251 0.21 +0.5 265,436 - yafY b0251 0.23 +0.4 265,519 - yafY b0251 0.42 -0.0 265,903 + ykfL b4627 0.57 -0.0 265,906 - ykfL b4627 0.58 -1.5 265,943 - ykfL b4627 0.75 -0.0 265,943 - ykfL b4627 0.75 -0.0 265,945 + ykfL b4627 0.76 +0.5 265,945 + ykfL b4627 0.76 -0.5 265,951 - ykfL b4627 0.78 -0.4 266,018 + +0.2 266,018 + +0.0 266,187 + +0.1 266,217 - +0.5 266,258 + -0.1 266,267 - -0.8 266,267 - -0.2 266,267 - -0.3 266,353 - +0.3 266,433 + -0.6 266,436 - -1.0 266,449 + -0.5 266,464 - +0.4 266,569 - yafZ b0252 0.20 -0.2 266,668 - yafZ b0252 0.32 +0.7 266,773 - yafZ b0252 0.44 -0.2
Or see this region's nucleotide sequence