Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjeJ and yjeK are separated by 394 nucleotides yjeK and efp are separated by 41 nucleotides efp and ecnA are separated by 51 nucleotides ecnA and ecnB are separated by 110 nucleotides
b4145: yjeJ - hypothetical protein (NCBI), at 4,371,388 to 4,372,257
yjeJ
b4146: yjeK - predicted lysine aminomutase (NCBI), at 4,372,652 to 4,373,680
yjeK
b4147: efp - elongation factor P (NCBI), at 4,373,722 to 4,374,288
efp
b4410: ecnA - entericidin A membrane lipoprotein, antidote entericidin B (NCBI), at 4,374,340 to 4,374,465
ecnA
b4411: ecnB - entericidin B membrane lipoprotein (NCBI), at 4,374,576 to 4,374,722
ecnB
Position (kb)
4372
4373
4374 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4371.656 kb on + strand, within yjeJ at 4371.656 kb on + strand, within yjeJ at 4371.670 kb on - strand, within yjeJ at 4371.694 kb on + strand, within yjeJ at 4371.694 kb on + strand, within yjeJ at 4371.708 kb on + strand, within yjeJ at 4371.708 kb on + strand, within yjeJ at 4371.718 kb on - strand, within yjeJ at 4371.739 kb on + strand, within yjeJ at 4371.740 kb on + strand, within yjeJ at 4371.740 kb on + strand, within yjeJ at 4371.747 kb on + strand, within yjeJ at 4371.748 kb on - strand, within yjeJ at 4371.755 kb on + strand, within yjeJ at 4371.772 kb on + strand, within yjeJ at 4371.795 kb on + strand, within yjeJ at 4371.813 kb on - strand, within yjeJ at 4371.813 kb on - strand, within yjeJ at 4371.851 kb on + strand, within yjeJ at 4371.851 kb on + strand, within yjeJ at 4371.851 kb on + strand, within yjeJ at 4371.869 kb on + strand, within yjeJ at 4371.869 kb on + strand, within yjeJ at 4371.895 kb on - strand, within yjeJ at 4371.903 kb on - strand, within yjeJ at 4371.945 kb on + strand, within yjeJ at 4371.945 kb on + strand, within yjeJ at 4371.945 kb on + strand, within yjeJ at 4371.953 kb on + strand, within yjeJ at 4371.953 kb on + strand, within yjeJ at 4371.961 kb on - strand, within yjeJ at 4372.045 kb on + strand, within yjeJ at 4372.045 kb on + strand, within yjeJ at 4372.045 kb on + strand, within yjeJ at 4372.051 kb on + strand, within yjeJ at 4372.051 kb on + strand, within yjeJ at 4372.053 kb on - strand, within yjeJ at 4372.053 kb on - strand, within yjeJ at 4372.055 kb on + strand, within yjeJ at 4372.055 kb on + strand, within yjeJ at 4372.055 kb on + strand, within yjeJ at 4372.055 kb on + strand, within yjeJ at 4372.055 kb on + strand, within yjeJ at 4372.055 kb on + strand, within yjeJ at 4372.065 kb on + strand, within yjeJ at 4372.070 kb on + strand, within yjeJ at 4372.080 kb on + strand, within yjeJ at 4372.087 kb on - strand, within yjeJ at 4372.110 kb on + strand, within yjeJ at 4372.112 kb on + strand, within yjeJ at 4372.112 kb on + strand, within yjeJ at 4372.114 kb on + strand, within yjeJ at 4372.115 kb on + strand, within yjeJ at 4372.122 kb on + strand, within yjeJ at 4372.122 kb on + strand, within yjeJ at 4372.124 kb on + strand, within yjeJ at 4372.124 kb on + strand, within yjeJ at 4372.131 kb on + strand, within yjeJ at 4372.131 kb on + strand, within yjeJ at 4372.134 kb on + strand, within yjeJ at 4372.143 kb on + strand, within yjeJ at 4372.150 kb on - strand, within yjeJ at 4372.160 kb on + strand, within yjeJ at 4372.213 kb on + strand at 4372.246 kb on + strand at 4372.247 kb on + strand at 4372.247 kb on + strand at 4372.267 kb on + strand at 4372.267 kb on + strand at 4372.409 kb on - strand at 4372.436 kb on - strand at 4372.451 kb on + strand at 4372.490 kb on - strand at 4372.503 kb on - strand at 4372.503 kb on - strand at 4372.503 kb on - strand at 4372.517 kb on - strand at 4372.518 kb on + strand at 4372.523 kb on - strand at 4372.533 kb on + strand at 4372.533 kb on + strand at 4372.547 kb on - strand at 4372.547 kb on - strand at 4372.547 kb on - strand at 4372.557 kb on - strand at 4372.557 kb on - strand at 4372.559 kb on - strand at 4372.559 kb on - strand at 4372.569 kb on - strand at 4372.569 kb on - strand at 4372.589 kb on + strand at 4372.589 kb on + strand at 4372.589 kb on + strand at 4372.596 kb on + strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.597 kb on - strand at 4372.651 kb on + strand at 4372.659 kb on - strand at 4372.674 kb on - strand at 4372.674 kb on - strand at 4372.731 kb on - strand at 4372.731 kb on - strand at 4372.801 kb on - strand, within yjeK at 4372.801 kb on - strand, within yjeK at 4372.818 kb on - strand, within yjeK at 4372.874 kb on - strand, within yjeK at 4372.876 kb on - strand, within yjeK at 4372.912 kb on + strand, within yjeK at 4372.915 kb on - strand, within yjeK at 4373.029 kb on + strand, within yjeK at 4373.029 kb on + strand, within yjeK at 4373.140 kb on - strand, within yjeK at 4373.140 kb on - strand, within yjeK at 4373.221 kb on + strand, within yjeK at 4373.255 kb on + strand, within yjeK at 4373.313 kb on - strand, within yjeK at 4373.387 kb on + strand, within yjeK at 4373.609 kb on + strand at 4373.609 kb on + strand at 4373.609 kb on + strand at 4373.695 kb on - strand at 4374.270 kb on + strand at 4374.303 kb on + strand at 4374.303 kb on + strand at 4374.336 kb on + strand at 4374.401 kb on - strand, within ecnA at 4374.646 kb on - strand, within ecnB at 4374.668 kb on - strand, within ecnB at 4374.668 kb on - strand, within ecnB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 4,371,656 + yjeJ b4145 0.31 -0.2 4,371,656 + yjeJ b4145 0.31 +0.2 4,371,670 - yjeJ b4145 0.32 -0.2 4,371,694 + yjeJ b4145 0.35 -0.8 4,371,694 + yjeJ b4145 0.35 +1.6 4,371,708 + yjeJ b4145 0.37 -0.6 4,371,708 + yjeJ b4145 0.37 +0.2 4,371,718 - yjeJ b4145 0.38 +0.8 4,371,739 + yjeJ b4145 0.40 +0.7 4,371,740 + yjeJ b4145 0.40 +0.2 4,371,740 + yjeJ b4145 0.40 -1.4 4,371,747 + yjeJ b4145 0.41 -0.8 4,371,748 - yjeJ b4145 0.41 -0.9 4,371,755 + yjeJ b4145 0.42 -1.5 4,371,772 + yjeJ b4145 0.44 -0.4 4,371,795 + yjeJ b4145 0.47 -0.4 4,371,813 - yjeJ b4145 0.49 +0.8 4,371,813 - yjeJ b4145 0.49 +0.8 4,371,851 + yjeJ b4145 0.53 -0.2 4,371,851 + yjeJ b4145 0.53 +0.2 4,371,851 + yjeJ b4145 0.53 +1.5 4,371,869 + yjeJ b4145 0.55 +0.8 4,371,869 + yjeJ b4145 0.55 +0.2 4,371,895 - yjeJ b4145 0.58 +1.8 4,371,903 - yjeJ b4145 0.59 +2.8 4,371,945 + yjeJ b4145 0.64 +0.5 4,371,945 + yjeJ b4145 0.64 -0.8 4,371,945 + yjeJ b4145 0.64 -0.4 4,371,953 + yjeJ b4145 0.65 +0.8 4,371,953 + yjeJ b4145 0.65 +1.2 4,371,961 - yjeJ b4145 0.66 -0.3 4,372,045 + yjeJ b4145 0.76 +1.8 4,372,045 + yjeJ b4145 0.76 +1.8 4,372,045 + yjeJ b4145 0.76 +1.8 4,372,051 + yjeJ b4145 0.76 +1.8 4,372,051 + yjeJ b4145 0.76 +1.8 4,372,053 - yjeJ b4145 0.76 +1.8 4,372,053 - yjeJ b4145 0.76 +0.8 4,372,055 + yjeJ b4145 0.77 +0.8 4,372,055 + yjeJ b4145 0.77 +0.2 4,372,055 + yjeJ b4145 0.77 +1.1 4,372,055 + yjeJ b4145 0.77 +2.8 4,372,055 + yjeJ b4145 0.77 -0.2 4,372,055 + yjeJ b4145 0.77 +0.9 4,372,065 + yjeJ b4145 0.78 +0.8 4,372,070 + yjeJ b4145 0.78 +0.5 4,372,080 + yjeJ b4145 0.80 +1.2 4,372,087 - yjeJ b4145 0.80 +1.8 4,372,110 + yjeJ b4145 0.83 +0.0 4,372,112 + yjeJ b4145 0.83 +1.8 4,372,112 + yjeJ b4145 0.83 -0.5 4,372,114 + yjeJ b4145 0.83 +1.2 4,372,115 + yjeJ b4145 0.84 -1.5 4,372,122 + yjeJ b4145 0.84 -1.6 4,372,122 + yjeJ b4145 0.84 +1.8 4,372,124 + yjeJ b4145 0.85 -1.4 4,372,124 + yjeJ b4145 0.85 -1.0 4,372,131 + yjeJ b4145 0.85 +0.1 4,372,131 + yjeJ b4145 0.85 +1.5 4,372,134 + yjeJ b4145 0.86 -0.8 4,372,143 + yjeJ b4145 0.87 +1.8 4,372,150 - yjeJ b4145 0.88 +0.2 4,372,160 + yjeJ b4145 0.89 -0.3 4,372,213 + +0.8 4,372,246 + +1.8 4,372,247 + +0.2 4,372,247 + +1.8 4,372,267 + +0.8 4,372,267 + +0.8 4,372,409 - -0.6 4,372,436 - +0.4 4,372,451 + -0.2 4,372,490 - +0.0 4,372,503 - +0.1 4,372,503 - -0.3 4,372,503 - -0.7 4,372,517 - -0.1 4,372,518 + -0.2 4,372,523 - +0.3 4,372,533 + +1.1 4,372,533 + +0.9 4,372,547 - -0.3 4,372,547 - -0.4 4,372,547 - -0.3 4,372,557 - +0.5 4,372,557 - -0.0 4,372,559 - +0.2 4,372,559 - +0.0 4,372,569 - +0.8 4,372,569 - +0.0 4,372,589 + +0.5 4,372,589 + -0.5 4,372,589 + -0.8 4,372,596 + -0.5 4,372,597 - +0.0 4,372,597 - -0.7 4,372,597 - -0.4 4,372,597 - +1.1 4,372,597 - +0.8 4,372,597 - -0.2 4,372,597 - +0.6 4,372,597 - +0.0 4,372,597 - +0.1 4,372,597 - -0.1 4,372,651 + +0.8 4,372,659 - -0.2 4,372,674 - -0.5 4,372,674 - -0.1 4,372,731 - -0.2 4,372,731 - +0.2 4,372,801 - yjeK b4146 0.14 -0.5 4,372,801 - yjeK b4146 0.14 +0.2 4,372,818 - yjeK b4146 0.16 -0.2 4,372,874 - yjeK b4146 0.22 +1.8 4,372,876 - yjeK b4146 0.22 -0.8 4,372,912 + yjeK b4146 0.25 +0.8 4,372,915 - yjeK b4146 0.26 +0.2 4,373,029 + yjeK b4146 0.37 +0.8 4,373,029 + yjeK b4146 0.37 +0.2 4,373,140 - yjeK b4146 0.47 -0.2 4,373,140 - yjeK b4146 0.47 -0.5 4,373,221 + yjeK b4146 0.55 +0.8 4,373,255 + yjeK b4146 0.59 -0.2 4,373,313 - yjeK b4146 0.64 -0.5 4,373,387 + yjeK b4146 0.71 -1.0 4,373,609 + +0.8 4,373,609 + +0.8 4,373,609 + +0.8 4,373,695 - -1.0 4,374,270 + -3.8 4,374,303 + -0.3 4,374,303 + -0.2 4,374,336 + -0.4 4,374,401 - ecnA b4410 0.48 -0.1 4,374,646 - ecnB b4411 0.48 -0.4 4,374,668 - ecnB b4411 0.63 -0.4 4,374,668 - ecnB b4411 0.63 -0.3
Or see this region's nucleotide sequence