Strain Fitness in Escherichia coli BW25113 around b3055

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygiF and htrG are separated by 241 nucleotideshtrG and cca are separated by 63 nucleotides b3054: ygiF - predicted adenylate cyclase (NCBI), at 3,197,686 to 3,198,987 ygiF b3055: htrG - predicted signal transduction protein (SH3 domain) (NCBI), at 3,199,229 to 3,199,849 htrG b3056: cca - fused tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase and phosphatase (NCBI), at 3,199,913 to 3,201,151 cca Position (kb) 3199 3200Strain fitness (log2 ratio) -2 -1 0 1 2at 3198.282 kb on - strand, within ygiFat 3198.321 kb on + strand, within ygiFat 3198.393 kb on - strand, within ygiFat 3198.393 kb on - strand, within ygiFat 3198.398 kb on - strand, within ygiFat 3198.398 kb on - strand, within ygiFat 3198.491 kb on + strand, within ygiFat 3198.552 kb on + strand, within ygiFat 3198.560 kb on - strand, within ygiFat 3198.561 kb on - strand, within ygiFat 3198.561 kb on - strand, within ygiFat 3198.711 kb on + strand, within ygiFat 3198.733 kb on + strand, within ygiFat 3198.733 kb on + strand, within ygiFat 3198.750 kb on + strand, within ygiFat 3198.750 kb on + strand, within ygiFat 3198.758 kb on - strand, within ygiFat 3198.824 kb on - strand, within ygiFat 3198.824 kb on - strand, within ygiFat 3198.828 kb on - strand, within ygiFat 3198.898 kb on - strandat 3198.900 kb on - strandat 3198.919 kb on + strandat 3198.991 kb on - strandat 3198.998 kb on + strandat 3198.998 kb on + strandat 3199.136 kb on + strandat 3199.139 kb on - strandat 3199.175 kb on - strandat 3199.180 kb on + strandat 3199.214 kb on + strandat 3199.274 kb on - strandat 3199.310 kb on + strand, within htrGat 3199.327 kb on - strand, within htrGat 3199.446 kb on + strand, within htrGat 3199.446 kb on + strand, within htrGat 3199.480 kb on + strand, within htrGat 3199.480 kb on + strand, within htrGat 3199.488 kb on - strand, within htrGat 3199.578 kb on - strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.721 kb on + strand, within htrGat 3199.721 kb on + strand, within htrGat 3199.724 kb on + strand, within htrGat 3199.724 kb on + strand, within htrGat 3199.747 kb on - strand, within htrGat 3199.759 kb on - strand, within htrGat 3199.759 kb on - strand, within htrGat 3199.771 kb on - strand, within htrGat 3199.771 kb on - strand, within htrGat 3199.778 kb on + strand, within htrGat 3199.781 kb on + strand, within htrGat 3199.781 kb on + strand, within htrGat 3199.878 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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3,198,282 - ygiF b3054 0.46 +0.8
3,198,321 + ygiF b3054 0.49 +0.2
3,198,393 - ygiF b3054 0.54 -0.6
3,198,393 - ygiF b3054 0.54 +0.1
3,198,398 - ygiF b3054 0.55 +0.1
3,198,398 - ygiF b3054 0.55 -0.2
3,198,491 + ygiF b3054 0.62 -0.1
3,198,552 + ygiF b3054 0.67 +0.5
3,198,560 - ygiF b3054 0.67 -0.4
3,198,561 - ygiF b3054 0.67 -0.4
3,198,561 - ygiF b3054 0.67 +0.2
3,198,711 + ygiF b3054 0.79 -0.4
3,198,733 + ygiF b3054 0.80 +0.4
3,198,733 + ygiF b3054 0.80 +0.3
3,198,750 + ygiF b3054 0.82 -0.8
3,198,750 + ygiF b3054 0.82 +0.4
3,198,758 - ygiF b3054 0.82 +0.5
3,198,824 - ygiF b3054 0.87 +0.2
3,198,824 - ygiF b3054 0.87 +0.1
3,198,828 - ygiF b3054 0.88 -0.4
3,198,898 - +0.4
3,198,900 - +0.2
3,198,919 + -1.7
3,198,991 - -0.0
3,198,998 + -0.2
3,198,998 + +0.5
3,199,136 + +2.2
3,199,139 - -1.9
3,199,175 - -0.7
3,199,180 + -1.1
3,199,214 + +0.1
3,199,274 - -2.4
3,199,310 + htrG b3055 0.13 -1.3
3,199,327 - htrG b3055 0.16 -0.4
3,199,446 + htrG b3055 0.35 -0.5
3,199,446 + htrG b3055 0.35 -1.8
3,199,480 + htrG b3055 0.40 -0.1
3,199,480 + htrG b3055 0.40 -1.1
3,199,488 - htrG b3055 0.42 -0.9
3,199,578 - htrG b3055 0.56 -1.8
3,199,716 + htrG b3055 0.78 -0.4
3,199,716 + htrG b3055 0.78 -0.4
3,199,716 + htrG b3055 0.78 -1.1
3,199,716 + htrG b3055 0.78 -0.8
3,199,721 + htrG b3055 0.79 -1.5
3,199,721 + htrG b3055 0.79 -0.8
3,199,724 + htrG b3055 0.80 -0.6
3,199,724 + htrG b3055 0.80 +0.8
3,199,747 - htrG b3055 0.83 -0.7
3,199,759 - htrG b3055 0.85 -1.2
3,199,759 - htrG b3055 0.85 -0.8
3,199,771 - htrG b3055 0.87 +0.7
3,199,771 - htrG b3055 0.87 -0.1
3,199,778 + htrG b3055 0.88 -0.6
3,199,781 + htrG b3055 0.89 -1.0
3,199,781 + htrG b3055 0.89 -1.1
3,199,878 - -0.7

Or see this region's nucleotide sequence