Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmA and napC are separated by 12 nucleotides napC and napB are separated by 9 nucleotides napB and napH overlap by 4 nucleotides napH and napG overlap by 14 nucleotides
b2201: ccmA - ATP binding protein of heme exporter A (VIMSS), at 2,295,043 to 2,295,666
ccmA
b2202: napC - nitrate reductase, cytochrome c-type, periplasmic (NCBI), at 2,295,679 to 2,296,281
napC
b2203: napB - cytochrome c-type protein (VIMSS), at 2,296,291 to 2,296,740
napB
b2204: napH - quinol dehydrogenase membrane component (NCBI), at 2,296,737 to 2,297,600
napH
b2205: napG - quinol dehydrogenase periplasmic component (NCBI), at 2,297,587 to 2,298,282
napG
Position (kb)
2296
2297 Strain fitness (log2 ratio)
-1
0
1 at 2295.401 kb on + strand, within ccmA at 2295.433 kb on - strand, within ccmA at 2295.476 kb on + strand, within ccmA at 2295.476 kb on + strand, within ccmA at 2295.516 kb on - strand, within ccmA at 2295.575 kb on - strand, within ccmA at 2295.650 kb on + strand at 2295.690 kb on + strand at 2295.722 kb on - strand at 2295.760 kb on + strand, within napC at 2295.760 kb on + strand, within napC at 2295.769 kb on + strand, within napC at 2295.806 kb on + strand, within napC at 2295.880 kb on + strand, within napC at 2295.921 kb on - strand, within napC at 2296.069 kb on - strand, within napC at 2296.154 kb on - strand, within napC at 2296.254 kb on + strand at 2296.254 kb on + strand at 2296.348 kb on + strand, within napB at 2296.348 kb on + strand, within napB at 2296.356 kb on + strand, within napB at 2296.356 kb on + strand, within napB at 2296.368 kb on + strand, within napB at 2296.431 kb on + strand, within napB at 2296.491 kb on + strand, within napB at 2296.491 kb on + strand, within napB at 2296.491 kb on - strand, within napB at 2296.545 kb on + strand, within napB at 2296.578 kb on + strand, within napB at 2296.578 kb on + strand, within napB at 2296.675 kb on + strand, within napB at 2296.695 kb on - strand, within napB at 2296.773 kb on + strand at 2296.773 kb on + strand at 2296.779 kb on - strand at 2296.880 kb on - strand, within napH at 2296.953 kb on + strand, within napH at 2297.095 kb on - strand, within napH at 2297.341 kb on + strand, within napH at 2297.444 kb on - strand, within napH at 2297.464 kb on + strand, within napH at 2297.476 kb on - strand, within napH at 2297.476 kb on - strand, within napH at 2297.517 kb on + strand at 2297.535 kb on - strand at 2297.535 kb on - strand at 2297.585 kb on - strand at 2297.585 kb on - strand at 2297.615 kb on - strand at 2297.676 kb on - strand, within napG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 2,295,401 + ccmA b2201 0.57 -1.5 2,295,433 - ccmA b2201 0.62 +0.0 2,295,476 + ccmA b2201 0.69 -1.3 2,295,476 + ccmA b2201 0.69 -0.0 2,295,516 - ccmA b2201 0.76 -0.5 2,295,575 - ccmA b2201 0.85 +0.3 2,295,650 + +0.6 2,295,690 + -0.6 2,295,722 - +0.2 2,295,760 + napC b2202 0.13 -0.7 2,295,760 + napC b2202 0.13 +0.7 2,295,769 + napC b2202 0.15 +1.1 2,295,806 + napC b2202 0.21 +0.1 2,295,880 + napC b2202 0.33 +0.1 2,295,921 - napC b2202 0.40 -0.2 2,296,069 - napC b2202 0.65 -0.1 2,296,154 - napC b2202 0.79 -0.3 2,296,254 + +0.1 2,296,254 + -0.7 2,296,348 + napB b2203 0.13 +0.0 2,296,348 + napB b2203 0.13 +0.1 2,296,356 + napB b2203 0.14 -0.1 2,296,356 + napB b2203 0.14 +0.8 2,296,368 + napB b2203 0.17 +0.1 2,296,431 + napB b2203 0.31 +0.6 2,296,491 + napB b2203 0.44 +0.5 2,296,491 + napB b2203 0.44 +0.7 2,296,491 - napB b2203 0.44 -0.4 2,296,545 + napB b2203 0.56 +0.8 2,296,578 + napB b2203 0.64 +1.0 2,296,578 + napB b2203 0.64 +0.3 2,296,675 + napB b2203 0.85 -0.0 2,296,695 - napB b2203 0.90 +0.3 2,296,773 + +0.4 2,296,773 + +0.1 2,296,779 - -1.3 2,296,880 - napH b2204 0.17 -0.4 2,296,953 + napH b2204 0.25 +0.1 2,297,095 - napH b2204 0.41 -0.5 2,297,341 + napH b2204 0.70 -0.3 2,297,444 - napH b2204 0.82 +0.0 2,297,464 + napH b2204 0.84 -0.2 2,297,476 - napH b2204 0.86 +0.5 2,297,476 - napH b2204 0.86 -0.5 2,297,517 + +0.6 2,297,535 - -0.0 2,297,535 - +0.5 2,297,585 - -0.2 2,297,585 - +0.4 2,297,615 - +0.3 2,297,676 - napG b2205 0.13 +0.0
Or see this region's nucleotide sequence