Strain Fitness in Escherichia coli BW25113 around b1959

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedI and yedA are separated by 172 nucleotidesyedA and vsr overlap by 12 nucleotidesvsr and dcm overlap by 20 nucleotides b1958: yedI - conserved inner membrane protein (NCBI), at 2,026,473 to 2,027,390 yedI b1959: yedA - predicted inner membrane protein (NCBI), at 2,027,563 to 2,028,483 yedA b1960: vsr - DNA mismatch endonuclease of very short patch repair (NCBI), at 2,028,472 to 2,028,942 vsr b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341 dcm Position (kb) 2027 2028 2029Strain fitness (log2 ratio) -2 -1 0 1at 2026.610 kb on - strand, within yedIat 2026.657 kb on - strand, within yedIat 2026.834 kb on + strand, within yedIat 2026.859 kb on - strand, within yedIat 2026.880 kb on + strand, within yedIat 2026.906 kb on - strand, within yedIat 2027.009 kb on - strand, within yedIat 2027.080 kb on - strand, within yedIat 2027.285 kb on - strand, within yedIat 2027.321 kb on - strandat 2027.339 kb on - strandat 2027.339 kb on - strandat 2027.416 kb on - strandat 2027.416 kb on - strandat 2027.424 kb on - strandat 2027.450 kb on - strandat 2027.459 kb on - strandat 2027.608 kb on + strandat 2027.686 kb on + strand, within yedAat 2027.723 kb on + strand, within yedAat 2028.038 kb on + strand, within yedAat 2028.081 kb on - strand, within yedAat 2028.322 kb on + strand, within yedAat 2028.398 kb on + strandat 2028.717 kb on + strand, within vsrat 2028.727 kb on - strand, within vsrat 2028.727 kb on - strand, within vsrat 2028.753 kb on + strand, within vsrat 2028.753 kb on + strand, within vsrat 2028.819 kb on - strand, within vsrat 2028.819 kb on - strand, within vsrat 2028.943 kb on + strandat 2028.966 kb on + strandat 2028.966 kb on - strandat 2029.342 kb on + strandat 2029.361 kb on - strand, within dcm

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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2,026,610 - yedI b1958 0.15 -0.5
2,026,657 - yedI b1958 0.20 +0.1
2,026,834 + yedI b1958 0.39 -0.5
2,026,859 - yedI b1958 0.42 -0.7
2,026,880 + yedI b1958 0.44 -0.7
2,026,906 - yedI b1958 0.47 -1.3
2,027,009 - yedI b1958 0.58 -0.2
2,027,080 - yedI b1958 0.66 +0.5
2,027,285 - yedI b1958 0.88 +0.3
2,027,321 - -0.4
2,027,339 - +0.1
2,027,339 - +0.4
2,027,416 - -0.7
2,027,416 - -0.3
2,027,424 - +0.1
2,027,450 - +0.0
2,027,459 - +0.2
2,027,608 + +0.2
2,027,686 + yedA b1959 0.13 +0.2
2,027,723 + yedA b1959 0.17 +0.8
2,028,038 + yedA b1959 0.52 +0.4
2,028,081 - yedA b1959 0.56 -0.2
2,028,322 + yedA b1959 0.82 +0.2
2,028,398 + -0.2
2,028,717 + vsr b1960 0.52 -0.2
2,028,727 - vsr b1960 0.54 +1.1
2,028,727 - vsr b1960 0.54 +0.5
2,028,753 + vsr b1960 0.60 +0.5
2,028,753 + vsr b1960 0.60 -0.2
2,028,819 - vsr b1960 0.74 -0.9
2,028,819 - vsr b1960 0.74 +0.0
2,028,943 + +0.5
2,028,966 + -0.6
2,028,966 - -0.4
2,029,342 + -2.0
2,029,361 - dcm b1961 0.31 -0.1

Or see this region's nucleotide sequence