Strain Fitness in Escherichia coli BW25113 around b1642

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhH and slyB are separated by 273 nucleotidesslyB and slyA are separated by 46 nucleotidesslyA and ydhI are separated by 200 nucleotidesydhI and b1644 are separated by 2 nucleotides b1640: ydhH - anhydro-N-acetylmuramic acid kinase (NCBI), at 1,716,517 to 1,717,626 ydhH b1641: slyB - outer membrane lipoprotein (NCBI), at 1,717,900 to 1,718,367 slyB b1642: slyA - transcriptional regulator for cryptic hemolysin (VIMSS), at 1,718,414 to 1,718,848 slyA b1643: ydhI - predicted inner membrane protein (NCBI), at 1,719,049 to 1,719,285 ydhI b1644: b1644 - putative membrane protein (VIMSS), at 1,719,288 to 1,720,145 b1644 Position (kb) 1718 1719Strain fitness (log2 ratio) -1 0 1 2at 1717.569 kb on + strandat 1717.569 kb on + strandat 1717.612 kb on - strandat 1717.661 kb on + strandat 1717.661 kb on + strandat 1717.696 kb on - strandat 1717.837 kb on + strandat 1717.934 kb on + strandat 1718.034 kb on - strand, within slyBat 1718.718 kb on - strand, within slyAat 1718.718 kb on - strand, within slyAat 1718.809 kb on - strandat 1719.062 kb on - strandat 1719.065 kb on + strandat 1719.078 kb on + strand, within ydhIat 1719.103 kb on - strand, within ydhIat 1719.163 kb on + strand, within ydhIat 1719.171 kb on - strand, within ydhIat 1719.171 kb on - strand, within ydhIat 1719.190 kb on + strand, within ydhIat 1719.211 kb on + strand, within ydhIat 1719.221 kb on - strand, within ydhIat 1719.559 kb on - strand, within b1644at 1719.779 kb on + strand, within b1644at 1719.779 kb on + strand, within b1644at 1719.786 kb on - strand, within b1644at 1719.786 kb on - strand, within b1644at 1719.832 kb on - strand, within b1644at 1719.832 kb on - strand, within b1644

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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1,717,569 + -0.2
1,717,569 + +1.0
1,717,612 - +0.7
1,717,661 + +0.7
1,717,661 + +0.8
1,717,696 - -0.3
1,717,837 + -0.3
1,717,934 + -0.1
1,718,034 - slyB b1641 0.29 +0.4
1,718,718 - slyA b1642 0.70 +0.2
1,718,718 - slyA b1642 0.70 +0.9
1,718,809 - -0.1
1,719,062 - -1.6
1,719,065 + +2.4
1,719,078 + ydhI b1643 0.12 +0.3
1,719,103 - ydhI b1643 0.23 -0.3
1,719,163 + ydhI b1643 0.48 -0.3
1,719,171 - ydhI b1643 0.51 -0.9
1,719,171 - ydhI b1643 0.51 -1.1
1,719,190 + ydhI b1643 0.59 +0.2
1,719,211 + ydhI b1643 0.68 +0.2
1,719,221 - ydhI b1643 0.73 +0.2
1,719,559 - b1644 b1644 0.32 +0.6
1,719,779 + b1644 b1644 0.57 +0.0
1,719,779 + b1644 b1644 0.57 +0.4
1,719,786 - b1644 b1644 0.58 +0.1
1,719,786 - b1644 b1644 0.58 -0.6
1,719,832 - b1644 b1644 0.63 +0.1
1,719,832 - b1644 b1644 0.63 -0.3

Or see this region's nucleotide sequence