Strain Fitness in Escherichia coli BW25113 around b1289

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfabI and ycjD are separated by 367 nucleotidesycjD and sapF are separated by 67 nucleotidessapF and sapD are separated by 1 nucleotides b1288: fabI - enoyl-(acyl carrier protein) reductase (NCBI), at 1,348,275 to 1,349,063 fabI b1289: ycjD - hypothetical protein (NCBI), at 1,349,431 to 1,349,784 ycjD b1290: sapF - predicted antimicrobial peptide transporter subunit (NCBI), at 1,349,852 to 1,350,658 sapF b1291: sapD - predicted antimicrobial peptide transporter subunit (NCBI), at 1,350,660 to 1,351,652 sapD Position (kb) 1349 1350Strain fitness (log2 ratio) -2 -1 0 1at 1349.183 kb on - strandat 1349.235 kb on + strandat 1349.243 kb on - strandat 1349.254 kb on + strandat 1349.254 kb on + strandat 1349.313 kb on + strandat 1349.335 kb on + strandat 1349.574 kb on - strand, within ycjDat 1349.577 kb on + strand, within ycjDat 1349.597 kb on - strand, within ycjDat 1349.645 kb on + strand, within ycjDat 1349.963 kb on - strand, within sapFat 1350.108 kb on + strand, within sapFat 1350.244 kb on + strandat 1350.244 kb on + strand, within sapFat 1350.286 kb on + strand, within sapFat 1350.294 kb on - strand, within sapFat 1350.550 kb on + strand, within sapFat 1350.656 kb on - strandat 1350.749 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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1,349,183 - +0.5
1,349,235 + -0.0
1,349,243 - +0.2
1,349,254 + +0.6
1,349,254 + -0.8
1,349,313 + +0.3
1,349,335 + -0.3
1,349,574 - ycjD b1289 0.40 +0.8
1,349,577 + ycjD b1289 0.41 +0.1
1,349,597 - ycjD b1289 0.47 +0.3
1,349,645 + ycjD b1289 0.60 -0.1
1,349,963 - sapF b1290 0.14 +0.4
1,350,108 + sapF b1290 0.32 -2.0
1,350,244 + -0.8
1,350,244 + sapF b1290 0.49 -0.6
1,350,286 + sapF b1290 0.54 -0.2
1,350,294 - sapF b1290 0.55 -0.6
1,350,550 + sapF b1290 0.86 +0.8
1,350,656 - -0.0
1,350,749 + -0.2

Or see this region's nucleotide sequence