Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciM and pyrF are separated by 193 nucleotides pyrF and yciH overlap by 1 nucleotides yciH and osmB are separated by 125 nucleotides osmB and yciT are separated by 268 nucleotides
b1280: yciM - hypothetical protein (NCBI), at 1,338,582 to 1,339,751
yciM
b1281: pyrF - orotidine 5'-phosphate decarboxylase (NCBI), at 1,339,945 to 1,340,682
pyrF
b1282: yciH - orf, hypothetical protein (VIMSS), at 1,340,682 to 1,341,008
yciH
b1283: osmB - lipoprotein (NCBI), at 1,341,134 to 1,341,352
osmB
b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370
yciT
Position (kb)
1339
1340
1341 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1339.902 kb on + strand at 1340.104 kb on + strand, within pyrF at 1340.104 kb on + strand, within pyrF at 1340.193 kb on + strand, within pyrF at 1340.295 kb on + strand, within pyrF at 1340.331 kb on + strand, within pyrF at 1340.455 kb on - strand, within pyrF at 1340.691 kb on + strand at 1340.708 kb on - strand at 1340.708 kb on - strand at 1340.748 kb on + strand, within yciH at 1340.781 kb on - strand, within yciH at 1340.902 kb on + strand, within yciH at 1340.937 kb on + strand, within yciH at 1340.940 kb on + strand, within yciH at 1340.986 kb on + strand at 1341.128 kb on - strand at 1341.179 kb on - strand, within osmB at 1341.179 kb on - strand, within osmB at 1341.187 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.203 kb on - strand, within osmB at 1341.211 kb on + strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.223 kb on - strand, within osmB at 1341.229 kb on - strand, within osmB at 1341.274 kb on + strand, within osmB at 1341.274 kb on + strand, within osmB at 1341.282 kb on - strand, within osmB at 1341.288 kb on + strand, within osmB at 1341.293 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.369 kb on - strand at 1341.369 kb on - strand at 1341.400 kb on - strand at 1341.421 kb on - strand at 1341.459 kb on - strand at 1341.465 kb on - strand at 1341.473 kb on - strand at 1341.473 kb on - strand at 1341.483 kb on - strand at 1341.494 kb on - strand at 1341.495 kb on - strand at 1341.495 kb on - strand at 1341.543 kb on - strand at 1341.585 kb on - strand at 1341.585 kb on - strand at 1341.609 kb on - strand at 1341.632 kb on - strand at 1341.645 kb on - strand at 1341.654 kb on + strand at 1341.654 kb on + strand at 1341.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 1,339,902 + +0.6 1,340,104 + pyrF b1281 0.22 -0.6 1,340,104 + pyrF b1281 0.22 -0.4 1,340,193 + pyrF b1281 0.34 -2.0 1,340,295 + pyrF b1281 0.47 -0.2 1,340,331 + pyrF b1281 0.52 -0.0 1,340,455 - pyrF b1281 0.69 -1.0 1,340,691 + -0.3 1,340,708 - -0.5 1,340,708 - -0.5 1,340,748 + yciH b1282 0.20 -1.2 1,340,781 - yciH b1282 0.30 -0.5 1,340,902 + yciH b1282 0.67 -0.6 1,340,937 + yciH b1282 0.78 -0.1 1,340,940 + yciH b1282 0.79 -0.2 1,340,986 + -0.9 1,341,128 - +0.1 1,341,179 - osmB b1283 0.21 -0.1 1,341,179 - osmB b1283 0.21 -1.3 1,341,187 - osmB b1283 0.24 +0.3 1,341,191 - osmB b1283 0.26 -0.2 1,341,191 - osmB b1283 0.26 -1.4 1,341,191 - osmB b1283 0.26 -0.9 1,341,203 - osmB b1283 0.32 +0.5 1,341,211 + osmB b1283 0.35 +1.8 1,341,214 - osmB b1283 0.37 +0.1 1,341,214 - osmB b1283 0.37 -0.0 1,341,214 - osmB b1283 0.37 -1.1 1,341,214 - osmB b1283 0.37 +0.1 1,341,214 - osmB b1283 0.37 -1.5 1,341,214 - osmB b1283 0.37 -0.4 1,341,219 - osmB b1283 0.39 -1.7 1,341,219 - osmB b1283 0.39 -0.5 1,341,219 - osmB b1283 0.39 -0.5 1,341,219 - osmB b1283 0.39 +1.6 1,341,219 - osmB b1283 0.39 +0.0 1,341,223 - osmB b1283 0.41 -0.5 1,341,229 - osmB b1283 0.43 -0.1 1,341,274 + osmB b1283 0.64 +1.8 1,341,274 + osmB b1283 0.64 +1.8 1,341,282 - osmB b1283 0.68 -1.2 1,341,288 + osmB b1283 0.70 +1.8 1,341,293 - osmB b1283 0.73 -0.1 1,341,296 - osmB b1283 0.74 +1.1 1,341,296 - osmB b1283 0.74 -0.5 1,341,296 - osmB b1283 0.74 +1.1 1,341,296 - osmB b1283 0.74 +1.3 1,341,369 - -1.2 1,341,369 - +0.4 1,341,400 - -0.5 1,341,421 - -0.1 1,341,459 - -1.4 1,341,465 - +0.2 1,341,473 - +0.6 1,341,473 - -0.8 1,341,483 - +0.5 1,341,494 - +0.3 1,341,495 - +0.2 1,341,495 - +0.1 1,341,543 - -1.1 1,341,585 - -0.2 1,341,585 - -0.5 1,341,609 - +0.2 1,341,632 - +0.4 1,341,645 - +0.2 1,341,654 + +0.2 1,341,654 + -0.2 1,341,656 + +1.2
Or see this region's nucleotide sequence