Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modA and modB overlap by 1 nucleotides modB and modC are separated by 2 nucleotides modC and ybhA are separated by 0 nucleotides ybhA and ybhE are separated by 154 nucleotides
b0763: modA - molybdate transporter subunit (NCBI), at 794,312 to 795,085
modA
b0764: modB - molybdate ABC transporter permease protein (NCBI), at 795,085 to 795,774
modB
b0765: modC - molybdate transporter subunit (NCBI), at 795,777 to 796,835
modC
b0766: ybhA - predicted hydrolase (NCBI), at 796,836 to 797,654
ybhA
b0767: ybhE - 6-phosphogluconolactonase (NCBI), at 797,809 to 798,804
ybhE
Position (kb) 795
796
797 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 794.800 kb on + strand, within modA at 794.876 kb on + strand, within modA at 794.989 kb on - strand, within modA at 795.088 kb on + strand at 795.113 kb on - strand at 795.153 kb on + strand at 795.230 kb on + strand, within modB at 795.330 kb on - strand, within modB at 795.549 kb on + strand, within modB at 795.549 kb on + strand at 795.552 kb on - strand, within modB at 795.638 kb on + strand, within modB at 795.638 kb on + strand, within modB at 795.642 kb on - strand, within modB at 795.644 kb on + strand, within modB at 795.646 kb on - strand, within modB at 795.695 kb on - strand, within modB at 795.811 kb on + strand at 795.811 kb on + strand at 795.820 kb on - strand at 795.820 kb on - strand at 796.082 kb on + strand, within modC at 796.082 kb on + strand, within modC at 796.218 kb on + strand, within modC at 796.234 kb on + strand, within modC at 796.234 kb on + strand, within modC at 796.352 kb on + strand, within modC at 796.369 kb on + strand, within modC at 796.369 kb on + strand, within modC at 796.377 kb on + strand, within modC at 796.377 kb on + strand, within modC at 796.377 kb on + strand, within modC at 796.377 kb on - strand, within modC at 796.377 kb on - strand, within modC at 796.590 kb on - strand, within modC at 796.862 kb on + strand at 797.012 kb on - strand, within ybhA at 797.022 kb on - strand, within ybhA at 797.100 kb on - strand, within ybhA at 797.122 kb on - strand, within ybhA at 797.289 kb on + strand, within ybhA at 797.318 kb on - strand, within ybhA at 797.334 kb on + strand, within ybhA at 797.334 kb on + strand at 797.380 kb on - strand, within ybhA at 797.565 kb on + strand, within ybhA at 797.565 kb on + strand, within ybhA at 797.592 kb on - strand at 797.615 kb on - strand at 797.615 kb on - strand at 797.702 kb on + strand at 797.702 kb on + strand at 797.775 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 794,800 + modA b0763 0.63 -0.3 794,876 + modA b0763 0.73 -0.6 794,989 - modA b0763 0.87 -1.8 795,088 + -0.1 795,113 - -0.6 795,153 + -1.4 795,230 + modB b0764 0.21 -1.3 795,330 - modB b0764 0.36 -2.6 795,549 + modB b0764 0.67 -0.2 795,549 + -1.5 795,552 - modB b0764 0.68 -1.5 795,638 + modB b0764 0.80 -1.0 795,638 + modB b0764 0.80 -0.9 795,642 - modB b0764 0.81 -1.0 795,644 + modB b0764 0.81 -1.8 795,646 - modB b0764 0.81 -1.4 795,695 - modB b0764 0.88 -3.3 795,811 + -2.5 795,811 + -2.1 795,820 - -1.3 795,820 - -1.8 796,082 + modC b0765 0.29 -1.1 796,082 + modC b0765 0.29 -1.2 796,218 + modC b0765 0.42 -1.1 796,234 + modC b0765 0.43 -0.9 796,234 + modC b0765 0.43 -1.5 796,352 + modC b0765 0.54 -1.6 796,369 + modC b0765 0.56 -3.2 796,369 + modC b0765 0.56 -0.6 796,377 + modC b0765 0.57 -0.9 796,377 + modC b0765 0.57 -1.0 796,377 + modC b0765 0.57 -1.2 796,377 - modC b0765 0.57 -1.1 796,377 - modC b0765 0.57 -1.8 796,590 - modC b0765 0.77 +0.1 796,862 + +0.5 797,012 - ybhA b0766 0.21 +0.0 797,022 - ybhA b0766 0.23 +0.0 797,100 - ybhA b0766 0.32 -0.3 797,122 - ybhA b0766 0.35 -0.2 797,289 + ybhA b0766 0.55 +0.2 797,318 - ybhA b0766 0.59 +0.3 797,334 + ybhA b0766 0.61 -0.2 797,334 + -0.6 797,380 - ybhA b0766 0.66 +0.2 797,565 + ybhA b0766 0.89 +0.0 797,565 + ybhA b0766 0.89 +0.3 797,592 - +0.0 797,615 - -0.4 797,615 - +0.2 797,702 + +0.2 797,702 + -0.5 797,775 + +0.0
Or see this region's nucleotide sequence