Strain Fitness in Escherichia coli BW25113 around b4493

Experiment: M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaldA and gapC are separated by 41 nucleotidesgapC and cybB are separated by 188 nucleotidescybB and rydC are separated by 10 nucleotidesrydC and ydcA are separated by 170 nucleotides b1415: aldA - Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data), at 1,486,256 to 1,487,695 aldA b4493: gapC - no description, at 1,487,737 to 1,488,737 gapC b1418: cybB - cytochrome b(561) (VIMSS), at 1,488,926 to 1,489,456 cybB b4597: rydC - sRNA regulator of yejABEF (RefSeq), at 1,489,467 to 1,489,530 rydC b1419: ydcA - hypothetical protein (NCBI), at 1,489,701 to 1,489,874 ydcA Position (kb) 1487 1488 1489Strain fitness (log2 ratio) -1 0 1at 1486.932 kb on + strand, within aldAat 1486.932 kb on + strand, within aldAat 1487.097 kb on + strand, within aldAat 1487.294 kb on - strand, within aldAat 1487.351 kb on - strand, within aldAat 1487.490 kb on + strand, within aldAat 1487.801 kb on + strandat 1487.801 kb on + strandat 1487.921 kb on + strand, within gapCat 1488.063 kb on - strand, within gapCat 1488.092 kb on - strand, within gapCat 1488.654 kb on - strandat 1488.684 kb on + strandat 1488.684 kb on + strandat 1488.827 kb on + strandat 1489.026 kb on + strand, within cybBat 1489.028 kb on + strand, within cybBat 1489.032 kb on + strand, within cybBat 1489.032 kb on + strand, within cybBat 1489.616 kb on + strandat 1489.627 kb on + strandat 1489.651 kb on + strandat 1489.659 kb on - strandat 1489.659 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM
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1,486,932 + aldA b1415 0.47 -0.0
1,486,932 + aldA b1415 0.47 -0.2
1,487,097 + aldA b1415 0.58 -0.5
1,487,294 - aldA b1415 0.72 +0.3
1,487,351 - aldA b1415 0.76 +0.7
1,487,490 + aldA b1415 0.86 -0.7
1,487,801 + -0.4
1,487,801 + -0.4
1,487,921 + gapC b4493 0.18 +0.1
1,488,063 - gapC b4493 0.33 +0.3
1,488,092 - gapC b4493 0.35 +0.5
1,488,654 - +0.3
1,488,684 + +0.2
1,488,684 + -0.6
1,488,827 + -0.3
1,489,026 + cybB b1418 0.19 -0.4
1,489,028 + cybB b1418 0.19 -0.2
1,489,032 + cybB b1418 0.20 -0.0
1,489,032 + cybB b1418 0.20 -0.2
1,489,616 + -1.0
1,489,627 + -0.1
1,489,651 + +0.1
1,489,659 - -0.2
1,489,659 - +0.4

Or see this region's nucleotide sequence