Strain Fitness in Escherichia coli BW25113 around b1400

Experiment: M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaX and paaY overlap by 19 nucleotidespaaY and ynbG are separated by 103 nucleotidesynbG and ydbA are separated by 161 nucleotides b1399: paaX - DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI), at 1,461,563 to 1,462,513 paaX b1400: paaY - predicted hexapeptide repeat acetyltransferase (NCBI), at 1,462,495 to 1,463,085 paaY b4674: ynbG - expressed protein (RefSeq), at 1,463,189 to 1,463,254 ynbG b4492: ydbA - no description, at 1,463,416 to 1,465,928 ydbA Position (kb) 1462 1463 1464Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1461.562 kb on + strandat 1461.562 kb on + strandat 1461.638 kb on + strandat 1461.687 kb on + strand, within paaXat 1461.873 kb on + strand, within paaXat 1461.873 kb on + strand, within paaXat 1461.938 kb on + strand, within paaXat 1461.978 kb on + strand, within paaXat 1462.399 kb on - strand, within paaXat 1462.521 kb on + strandat 1462.521 kb on + strandat 1462.975 kb on + strand, within paaYat 1462.975 kb on + strand, within paaYat 1462.988 kb on + strand, within paaYat 1463.000 kb on + strand, within paaYat 1463.053 kb on + strandat 1463.053 kb on + strandat 1463.080 kb on + strandat 1463.082 kb on + strandat 1463.499 kb on - strandat 1463.499 kb on - strandat 1463.754 kb on - strand, within ydbAat 1463.761 kb on - strand, within ydbAat 1463.762 kb on - strand, within ydbAat 1463.764 kb on - strand, within ydbAat 1463.764 kb on - strand, within ydbAat 1463.791 kb on + strand, within ydbAat 1463.801 kb on + strand, within ydbAat 1463.809 kb on - strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.821 kb on - strand, within ydbAat 1463.822 kb on - strand, within ydbAat 1463.874 kb on + strand, within ydbAat 1463.874 kb on + strand, within ydbAat 1463.924 kb on - strand, within ydbAat 1463.928 kb on - strand, within ydbAat 1463.932 kb on - strand, within ydbAat 1463.933 kb on - strand, within ydbAat 1463.971 kb on - strand, within ydbAat 1463.971 kb on - strand, within ydbAat 1463.989 kb on - strand, within ydbAat 1464.012 kb on - strand, within ydbAat 1464.023 kb on - strand, within ydbAat 1464.023 kb on - strand, within ydbAat 1464.026 kb on - strand, within ydbAat 1464.026 kb on - strand, within ydbA

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM
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1,461,562 + -0.1
1,461,562 + -0.1
1,461,638 + -0.8
1,461,687 + paaX b1399 0.13 +0.0
1,461,873 + paaX b1399 0.33 +0.3
1,461,873 + paaX b1399 0.33 +0.3
1,461,938 + paaX b1399 0.39 +0.4
1,461,978 + paaX b1399 0.44 +0.1
1,462,399 - paaX b1399 0.88 -0.1
1,462,521 + +0.8
1,462,521 + +0.0
1,462,975 + paaY b1400 0.81 +0.5
1,462,975 + paaY b1400 0.81 +0.3
1,462,988 + paaY b1400 0.83 +0.6
1,463,000 + paaY b1400 0.85 +0.6
1,463,053 + -0.6
1,463,053 + -0.3
1,463,080 + +0.6
1,463,082 + -0.4
1,463,499 - +1.6
1,463,499 - +0.6
1,463,754 - ydbA b4492 0.13 +3.1
1,463,761 - ydbA b4492 0.14 -0.3
1,463,762 - ydbA b4492 0.14 -1.4
1,463,764 - ydbA b4492 0.14 +1.6
1,463,764 - ydbA b4492 0.14 +0.6
1,463,791 + ydbA b4492 0.15 -0.4
1,463,801 + ydbA b4492 0.15 -2.4
1,463,809 - ydbA b4492 0.16 +1.0
1,463,813 + ydbA b4492 0.16 +0.6
1,463,813 + ydbA b4492 0.16 -2.4
1,463,813 + ydbA b4492 0.16 +0.2
1,463,813 + ydbA b4492 0.16 +0.3
1,463,821 - ydbA b4492 0.16 +2.0
1,463,822 - ydbA b4492 0.16 -0.0
1,463,874 + ydbA b4492 0.18 -0.9
1,463,874 + ydbA b4492 0.18 +0.7
1,463,924 - ydbA b4492 0.20 -0.2
1,463,928 - ydbA b4492 0.20 +0.1
1,463,932 - ydbA b4492 0.21 +0.2
1,463,933 - ydbA b4492 0.21 +0.2
1,463,971 - ydbA b4492 0.22 +0.3
1,463,971 - ydbA b4492 0.22 +0.2
1,463,989 - ydbA b4492 0.23 -0.3
1,464,012 - ydbA b4492 0.24 -1.4
1,464,023 - ydbA b4492 0.24 -0.1
1,464,023 - ydbA b4492 0.24 -1.0
1,464,026 - ydbA b4492 0.24 +0.0
1,464,026 - ydbA b4492 0.24 +1.1

Or see this region's nucleotide sequence