Strain Fitness in Escherichia coli BW25113 around b1370

Experiment: M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynaA and lomR are separated by 2 nucleotideslomR and insH-5 are separated by 147 nucleotidesinsH-5 and b1372 are separated by 322 nucleotides b1368: ynaA - Rac prophage; predicted tail protein (RefSeq), at 1,424,478 to 1,425,410 ynaA b4570: lomR - no description, at 1,425,413 to 1,425,622 lomR b1370: insH-5 - Rac prophage; IS5 transposase and trans-activator (NCBI), at 1,425,770 to 1,426,750 insH-5 b1372: b1372 - putative membrane protein (VIMSS), at 1,427,073 to 1,430,435 b1372 Position (kb) 1425 1426 1427Strain fitness (log2 ratio) -2 -1 0 1at 1424.862 kb on - strand, within ynaAat 1424.862 kb on - strand, within ynaAat 1424.869 kb on - strand, within ynaAat 1425.045 kb on + strand, within ynaAat 1425.045 kb on + strand, within ynaAat 1425.060 kb on + strand, within ynaAat 1425.178 kb on + strand, within ynaAat 1425.321 kb on - strandat 1425.377 kb on - strandat 1425.377 kb on - strandat 1425.496 kb on + strand, within lomRat 1425.548 kb on + strand, within lomRat 1425.574 kb on - strand, within lomRat 1426.405 kb on + strand, within insH-5at 1426.456 kb on - strand, within insH-5at 1426.658 kb on + strandat 1426.658 kb on + strandat 1426.738 kb on + strandat 1426.738 kb on + strandat 1426.746 kb on + strandat 1426.877 kb on + strandat 1426.954 kb on - strandat 1426.954 kb on - strandat 1427.144 kb on - strandat 1427.251 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 500 uM
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1,424,862 - ynaA b1368 0.41 -1.4
1,424,862 - ynaA b1368 0.41 +1.1
1,424,869 - ynaA b1368 0.42 -0.3
1,425,045 + ynaA b1368 0.61 +1.6
1,425,045 + ynaA b1368 0.61 +0.6
1,425,060 + ynaA b1368 0.62 +0.2
1,425,178 + ynaA b1368 0.75 +0.7
1,425,321 - -0.4
1,425,377 - -2.4
1,425,377 - -1.4
1,425,496 + lomR b4570 0.40 +0.4
1,425,548 + lomR b4570 0.64 +0.5
1,425,574 - lomR b4570 0.77 +0.2
1,426,405 + insH-5 b1370 0.65 +0.9
1,426,456 - insH-5 b1370 0.70 +0.2
1,426,658 + +0.6
1,426,658 + -0.5
1,426,738 + -0.8
1,426,738 + -0.6
1,426,746 + +0.4
1,426,877 + -0.1
1,426,954 - +0.4
1,426,954 - +0.7
1,427,144 - -0.7
1,427,251 - -0.5

Or see this region's nucleotide sequence