Strain Fitness in Escherichia coli BW25113 around b2734

Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmutS and pphB are separated by 105 nucleotidespphB and ygbI are separated by 50 nucleotides b2733: mutS - DNA mismatch repair protein (NCBI), at 2,855,115 to 2,857,676 mutS b2734: pphB - serine/threonine-specific protein phosphatase 2 (NCBI), at 2,857,782 to 2,858,438 pphB b2735: ygbI - predicted DNA-binding transcriptional regulator (RefSeq), at 2,858,489 to 2,859,256 ygbI Position (kb) 2857 2858 2859Strain fitness (log2 ratio) -2 -1 0 1 2at 2856.799 kb on + strand, within mutSat 2856.799 kb on + strand, within mutSat 2856.804 kb on + strand, within mutSat 2856.812 kb on - strand, within mutSat 2856.817 kb on - strand, within mutSat 2856.986 kb on + strand, within mutSat 2856.999 kb on - strand, within mutSat 2857.001 kb on + strand, within mutSat 2857.021 kb on - strand, within mutSat 2857.089 kb on - strand, within mutSat 2857.308 kb on - strand, within mutSat 2857.378 kb on - strand, within mutSat 2857.378 kb on - strand, within mutSat 2857.408 kb on - strand, within mutSat 2857.408 kb on - strand, within mutSat 2857.453 kb on - strandat 2857.453 kb on - strandat 2857.512 kb on + strandat 2857.543 kb on + strandat 2857.566 kb on + strandat 2857.570 kb on - strandat 2857.575 kb on - strandat 2857.577 kb on + strandat 2857.591 kb on - strandat 2857.617 kb on + strandat 2857.621 kb on + strandat 2857.621 kb on + strandat 2857.621 kb on + strandat 2857.621 kb on + strandat 2857.621 kb on + strandat 2857.631 kb on + strandat 2857.649 kb on + strandat 2857.665 kb on + strandat 2857.665 kb on + strandat 2857.719 kb on + strandat 2857.743 kb on + strandat 2857.743 kb on + strandat 2857.747 kb on + strandat 2857.780 kb on - strandat 2857.784 kb on + strandat 2857.785 kb on - strandat 2857.786 kb on + strandat 2857.786 kb on + strandat 2857.787 kb on + strandat 2857.788 kb on + strandat 2857.794 kb on - strandat 2857.795 kb on - strandat 2857.829 kb on + strandat 2857.829 kb on + strandat 2857.838 kb on - strandat 2857.868 kb on + strand, within pphBat 2857.877 kb on - strand, within pphBat 2857.877 kb on - strand, within pphBat 2857.942 kb on + strand, within pphBat 2857.950 kb on - strand, within pphBat 2857.953 kb on - strand, within pphBat 2857.955 kb on - strand, within pphBat 2857.957 kb on + strand, within pphBat 2857.960 kb on - strand, within pphBat 2857.961 kb on - strand, within pphBat 2857.965 kb on + strand, within pphBat 2857.965 kb on - strand, within pphBat 2857.965 kb on - strand, within pphBat 2857.965 kb on - strand, within pphBat 2857.965 kb on - strand, within pphBat 2857.973 kb on - strand, within pphBat 2857.973 kb on - strand, within pphBat 2857.993 kb on - strand, within pphBat 2857.993 kb on - strand, within pphBat 2858.008 kb on - strand, within pphBat 2858.026 kb on + strand, within pphBat 2858.056 kb on + strand, within pphBat 2858.067 kb on + strand, within pphBat 2858.069 kb on + strand, within pphBat 2858.146 kb on + strand, within pphBat 2858.146 kb on + strand, within pphBat 2858.210 kb on + strand, within pphBat 2858.261 kb on - strand, within pphBat 2858.275 kb on - strand, within pphBat 2858.397 kb on - strandat 2858.405 kb on + strandat 2858.457 kb on + strandat 2858.457 kb on - strandat 2858.466 kb on - strandat 2858.471 kb on - strandat 2858.471 kb on - strandat 2858.473 kb on - strandat 2858.476 kb on - strandat 2858.514 kb on + strandat 2858.518 kb on + strandat 2858.568 kb on + strand, within ygbIat 2858.568 kb on + strand, within ygbIat 2858.618 kb on - strand, within ygbIat 2858.719 kb on + strand, within ygbIat 2858.822 kb on - strand, within ygbIat 2858.827 kb on - strand, within ygbIat 2858.827 kb on - strand, within ygbIat 2858.912 kb on - strand, within ygbIat 2859.114 kb on - strand, within ygbIat 2859.160 kb on - strand, within ygbI

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
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2,856,799 + mutS b2733 0.66 +0.7
2,856,799 + mutS b2733 0.66 -0.9
2,856,804 + mutS b2733 0.66 -0.4
2,856,812 - mutS b2733 0.66 +0.0
2,856,817 - mutS b2733 0.66 -0.1
2,856,986 + mutS b2733 0.73 -0.1
2,856,999 - mutS b2733 0.74 +0.3
2,857,001 + mutS b2733 0.74 +0.6
2,857,021 - mutS b2733 0.74 -0.1
2,857,089 - mutS b2733 0.77 -0.5
2,857,308 - mutS b2733 0.86 +0.1
2,857,378 - mutS b2733 0.88 +0.8
2,857,378 - mutS b2733 0.88 -1.0
2,857,408 - mutS b2733 0.90 +0.1
2,857,408 - mutS b2733 0.90 +0.1
2,857,453 - +0.3
2,857,453 - +0.9
2,857,512 + -0.2
2,857,543 + -0.5
2,857,566 + -0.1
2,857,570 - +0.0
2,857,575 - -2.3
2,857,577 + -1.8
2,857,591 - -0.6
2,857,617 + -0.0
2,857,621 + -0.5
2,857,621 + +0.0
2,857,621 + -0.3
2,857,621 + -1.6
2,857,621 + +0.2
2,857,631 + -1.0
2,857,649 + -0.0
2,857,665 + -0.6
2,857,665 + +0.6
2,857,719 + +0.1
2,857,743 + -1.4
2,857,743 + +1.0
2,857,747 + -1.3
2,857,780 - -0.6
2,857,784 + +1.6
2,857,785 - +2.6
2,857,786 + +2.6
2,857,786 + +1.0
2,857,787 + +0.2
2,857,788 + -0.0
2,857,794 - -1.6
2,857,795 - +1.0
2,857,829 + +2.6
2,857,829 + +1.6
2,857,838 - +1.6
2,857,868 + pphB b2734 0.13 +2.6
2,857,877 - pphB b2734 0.14 -0.3
2,857,877 - pphB b2734 0.14 +1.2
2,857,942 + pphB b2734 0.24 +2.6
2,857,950 - pphB b2734 0.26 +0.3
2,857,953 - pphB b2734 0.26 +0.9
2,857,955 - pphB b2734 0.26 +0.5
2,857,957 + pphB b2734 0.27 +2.6
2,857,960 - pphB b2734 0.27 +1.0
2,857,961 - pphB b2734 0.27 +0.3
2,857,965 + pphB b2734 0.28 +2.6
2,857,965 - pphB b2734 0.28 -0.1
2,857,965 - pphB b2734 0.28 +1.2
2,857,965 - pphB b2734 0.28 +1.4
2,857,965 - pphB b2734 0.28 +1.0
2,857,973 - pphB b2734 0.29 +0.2
2,857,973 - pphB b2734 0.29 +0.6
2,857,993 - pphB b2734 0.32 -0.6
2,857,993 - pphB b2734 0.32 +1.6
2,858,008 - pphB b2734 0.34 -0.8
2,858,026 + pphB b2734 0.37 +2.6
2,858,056 + pphB b2734 0.42 +1.0
2,858,067 + pphB b2734 0.43 +2.6
2,858,069 + pphB b2734 0.44 +1.0
2,858,146 + pphB b2734 0.55 +2.6
2,858,146 + pphB b2734 0.55 +2.6
2,858,210 + pphB b2734 0.65 +1.6
2,858,261 - pphB b2734 0.73 +1.0
2,858,275 - pphB b2734 0.75 +2.6
2,858,397 - +1.9
2,858,405 + -0.6
2,858,457 + -1.8
2,858,457 - +0.3
2,858,466 - -0.6
2,858,471 - -0.3
2,858,471 - -0.8
2,858,473 - -0.2
2,858,476 - +0.1
2,858,514 + -0.9
2,858,518 + -1.1
2,858,568 + ygbI b2735 0.10 +0.2
2,858,568 + ygbI b2735 0.10 +0.9
2,858,618 - ygbI b2735 0.17 -0.3
2,858,719 + ygbI b2735 0.30 -1.1
2,858,822 - ygbI b2735 0.43 -0.2
2,858,827 - ygbI b2735 0.44 -0.8
2,858,827 - ygbI b2735 0.44 -1.8
2,858,912 - ygbI b2735 0.55 -0.5
2,859,114 - ygbI b2735 0.81 -0.9
2,859,160 - ygbI b2735 0.87 -0.1

Or see this region's nucleotide sequence