Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ypjA and pinH are separated by 338 nucleotides pinH and ypjC are separated by 333 nucleotides ypjC and ileY are separated by 750 nucleotides
b2647: ypjA - putative ATP-binding component of a transport system (VIMSS), at 2,776,168 to 2,780,748
ypjA
b2648: pinH - predicted invertase fragment (pseudogene) (RefSeq), at 2,781,087 to 2,781,326
pinH
b2650: ypjC - hypothetical protein (RefSeq), at 2,781,660 to 2,783,033
ypjC
b2652: ileY - tRNA-Ile (NCBI), at 2,783,784 to 2,783,859
ileY
Position (kb)
2781
2782
2783
2784 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2780.661 kb on + strand at 2780.666 kb on + strand at 2780.683 kb on + strand at 2780.687 kb on - strand at 2780.691 kb on - strand at 2780.693 kb on + strand at 2780.704 kb on + strand at 2780.704 kb on + strand at 2780.729 kb on - strand at 2780.731 kb on - strand at 2780.741 kb on - strand at 2780.741 kb on - strand at 2780.777 kb on - strand at 2780.782 kb on - strand at 2780.782 kb on - strand at 2780.782 kb on - strand at 2780.790 kb on - strand at 2780.801 kb on - strand at 2780.801 kb on - strand at 2780.801 kb on - strand at 2780.805 kb on - strand at 2780.813 kb on - strand at 2780.813 kb on - strand at 2780.818 kb on - strand at 2780.820 kb on + strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.838 kb on - strand at 2780.862 kb on + strand at 2780.862 kb on + strand at 2780.862 kb on + strand at 2780.862 kb on + strand at 2780.863 kb on + strand at 2780.865 kb on + strand at 2780.865 kb on + strand at 2780.865 kb on + strand at 2780.870 kb on + strand at 2780.870 kb on + strand at 2780.872 kb on + strand at 2780.872 kb on + strand at 2780.945 kb on + strand at 2780.952 kb on + strand at 2780.952 kb on + strand at 2780.963 kb on - strand at 2781.023 kb on - strand at 2781.137 kb on - strand, within pinH at 2781.154 kb on + strand, within pinH at 2781.158 kb on - strand, within pinH at 2781.162 kb on - strand, within pinH at 2781.163 kb on - strand, within pinH at 2781.202 kb on + strand, within pinH at 2781.205 kb on + strand, within pinH at 2781.207 kb on + strand, within pinH at 2781.208 kb on - strand, within pinH at 2781.210 kb on - strand, within pinH at 2781.210 kb on - strand, within pinH at 2781.210 kb on - strand, within pinH at 2781.210 kb on - strand, within pinH at 2781.210 kb on - strand, within pinH at 2781.213 kb on - strand, within pinH at 2781.215 kb on - strand, within pinH at 2781.220 kb on - strand, within pinH at 2781.220 kb on - strand, within pinH at 2781.222 kb on + strand, within pinH at 2781.224 kb on + strand, within pinH at 2781.224 kb on + strand, within pinH at 2781.224 kb on + strand, within pinH at 2781.224 kb on + strand, within pinH at 2781.228 kb on - strand, within pinH at 2781.228 kb on - strand, within pinH at 2781.230 kb on - strand, within pinH at 2781.230 kb on - strand, within pinH at 2781.232 kb on - strand, within pinH at 2781.232 kb on - strand, within pinH at 2781.232 kb on - strand, within pinH at 2781.232 kb on - strand, within pinH at 2781.232 kb on - strand, within pinH at 2781.232 kb on - strand, within pinH at 2781.240 kb on - strand, within pinH at 2781.250 kb on + strand, within pinH at 2781.250 kb on + strand, within pinH at 2781.258 kb on + strand, within pinH at 2781.258 kb on + strand, within pinH at 2781.258 kb on + strand, within pinH at 2781.258 kb on + strand, within pinH at 2781.258 kb on + strand, within pinH at 2781.259 kb on - strand, within pinH at 2781.260 kb on + strand, within pinH at 2781.266 kb on - strand, within pinH at 2781.266 kb on - strand, within pinH at 2781.271 kb on - strand, within pinH at 2781.277 kb on + strand, within pinH at 2781.281 kb on + strand, within pinH at 2781.281 kb on + strand, within pinH at 2781.289 kb on - strand, within pinH at 2781.289 kb on - strand, within pinH at 2781.332 kb on - strand at 2781.336 kb on + strand at 2781.336 kb on + strand at 2781.336 kb on + strand at 2781.337 kb on + strand at 2781.344 kb on + strand at 2781.345 kb on - strand at 2781.346 kb on - strand at 2781.357 kb on + strand at 2781.357 kb on + strand at 2781.357 kb on + strand at 2781.357 kb on + strand at 2781.359 kb on + strand at 2781.372 kb on + strand at 2781.372 kb on + strand at 2781.375 kb on + strand at 2781.380 kb on + strand at 2781.380 kb on + strand at 2781.380 kb on + strand at 2781.380 kb on + strand at 2781.380 kb on + strand at 2781.380 kb on + strand at 2781.425 kb on + strand at 2781.537 kb on + strand at 2781.573 kb on + strand at 2781.591 kb on - strand at 2781.631 kb on - strand at 2781.631 kb on - strand at 2781.683 kb on - strand at 2781.683 kb on - strand at 2781.697 kb on - strand at 2781.697 kb on - strand at 2781.697 kb on - strand at 2781.699 kb on + strand at 2781.707 kb on - strand at 2781.707 kb on - strand at 2781.707 kb on - strand at 2781.707 kb on - strand at 2781.722 kb on - strand at 2781.739 kb on - strand at 2781.742 kb on + strand at 2781.922 kb on - strand, within ypjC at 2781.925 kb on - strand, within ypjC at 2783.288 kb on - strand at 2783.305 kb on - strand at 2783.305 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.318 kb on - strand at 2783.320 kb on - strand at 2783.321 kb on - strand at 2783.321 kb on - strand at 2783.350 kb on + strand at 2783.360 kb on + strand at 2783.360 kb on + strand at 2783.413 kb on + strand at 2783.413 kb on + strand at 2783.413 kb on + strand at 2783.413 kb on + strand at 2783.413 kb on + strand at 2783.418 kb on + strand at 2783.421 kb on - strand at 2783.423 kb on + strand at 2783.467 kb on - strand at 2783.638 kb on - strand at 2783.638 kb on - strand at 2783.643 kb on - strand at 2783.643 kb on - strand at 2783.650 kb on - strand at 2783.661 kb on - strand at 2783.661 kb on - strand at 2783.663 kb on - strand at 2783.664 kb on - strand at 2783.729 kb on - strand at 2783.731 kb on + strand at 2783.734 kb on + strand at 2783.737 kb on + strand at 2783.743 kb on + strand at 2783.743 kb on + strand at 2783.744 kb on + strand at 2783.745 kb on + strand at 2783.751 kb on - strand at 2783.751 kb on - strand at 2783.752 kb on + strand at 2783.756 kb on + strand at 2783.796 kb on - strand, within ileY at 2783.821 kb on - strand, within ileY at 2783.838 kb on - strand, within ileY at 2783.843 kb on - strand, within ileY at 2783.843 kb on - strand, within ileY at 2783.843 kb on - strand, within ileY at 2783.847 kb on + strand, within ileY at 2783.847 kb on + strand, within ileY at 2783.864 kb on - strand at 2783.864 kb on - strand at 2783.864 kb on - strand at 2783.888 kb on - strand at 2783.888 kb on - strand at 2783.952 kb on - strand at 2783.956 kb on - strand at 2783.967 kb on - strand at 2783.979 kb on + strand at 2783.986 kb on - strand at 2783.994 kb on - strand at 2784.011 kb on - strand at 2784.024 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 2,780,661 + +0.3 2,780,666 + +0.6 2,780,683 + +0.6 2,780,687 - +2.6 2,780,691 - +2.6 2,780,693 + +1.6 2,780,704 + +2.6 2,780,704 + +1.6 2,780,729 - +1.0 2,780,731 - +2.6 2,780,741 - +0.2 2,780,741 - -1.1 2,780,777 - -0.9 2,780,782 - -0.2 2,780,782 - +0.3 2,780,782 - +1.2 2,780,790 - -2.5 2,780,801 - -0.6 2,780,801 - +0.6 2,780,801 - +0.1 2,780,805 - +0.3 2,780,813 - -0.1 2,780,813 - -0.2 2,780,818 - -0.8 2,780,820 + +0.3 2,780,824 - +0.7 2,780,824 - +0.0 2,780,824 - +1.0 2,780,838 - +2.6 2,780,838 - +1.0 2,780,838 - -0.8 2,780,838 - +1.4 2,780,838 - -0.7 2,780,838 - +0.7 2,780,838 - -1.1 2,780,838 - -0.3 2,780,838 - +1.6 2,780,862 + +1.4 2,780,862 + +0.0 2,780,862 + +0.3 2,780,862 + +0.4 2,780,863 + +0.5 2,780,865 + -1.1 2,780,865 + +0.0 2,780,865 + -2.3 2,780,870 + -0.4 2,780,870 + +0.3 2,780,872 + +2.0 2,780,872 + +0.3 2,780,945 + +0.7 2,780,952 + +0.6 2,780,952 + -2.0 2,780,963 - +2.6 2,781,023 - +2.6 2,781,137 - pinH b2648 0.21 +2.6 2,781,154 + pinH b2648 0.28 +2.6 2,781,158 - pinH b2648 0.30 -0.7 2,781,162 - pinH b2648 0.31 +0.3 2,781,163 - pinH b2648 0.32 +0.6 2,781,202 + pinH b2648 0.48 +2.6 2,781,205 + pinH b2648 0.49 +2.6 2,781,207 + pinH b2648 0.50 +2.6 2,781,208 - pinH b2648 0.50 +0.0 2,781,210 - pinH b2648 0.51 +1.0 2,781,210 - pinH b2648 0.51 -0.1 2,781,210 - pinH b2648 0.51 +2.0 2,781,210 - pinH b2648 0.51 +0.2 2,781,210 - pinH b2648 0.51 +0.5 2,781,213 - pinH b2648 0.53 -0.3 2,781,215 - pinH b2648 0.53 -0.3 2,781,220 - pinH b2648 0.55 -0.1 2,781,220 - pinH b2648 0.55 -0.2 2,781,222 + pinH b2648 0.56 -0.2 2,781,224 + pinH b2648 0.57 +1.8 2,781,224 + pinH b2648 0.57 +0.3 2,781,224 + pinH b2648 0.57 +0.2 2,781,224 + pinH b2648 0.57 +1.6 2,781,228 - pinH b2648 0.59 -0.1 2,781,228 - pinH b2648 0.59 -1.3 2,781,230 - pinH b2648 0.60 +0.1 2,781,230 - pinH b2648 0.60 -0.3 2,781,232 - pinH b2648 0.60 +0.7 2,781,232 - pinH b2648 0.60 -0.4 2,781,232 - pinH b2648 0.60 -2.4 2,781,232 - pinH b2648 0.60 +0.0 2,781,232 - pinH b2648 0.60 +0.4 2,781,232 - pinH b2648 0.60 -1.1 2,781,240 - pinH b2648 0.64 +1.8 2,781,250 + pinH b2648 0.68 +1.9 2,781,250 + pinH b2648 0.68 +0.4 2,781,258 + pinH b2648 0.71 +0.5 2,781,258 + pinH b2648 0.71 +0.8 2,781,258 + pinH b2648 0.71 +0.3 2,781,258 + pinH b2648 0.71 -0.9 2,781,258 + pinH b2648 0.71 -0.2 2,781,259 - pinH b2648 0.72 +1.9 2,781,260 + pinH b2648 0.72 -0.4 2,781,266 - pinH b2648 0.75 -0.5 2,781,266 - pinH b2648 0.75 -0.4 2,781,271 - pinH b2648 0.77 -1.2 2,781,277 + pinH b2648 0.79 -0.4 2,781,281 + pinH b2648 0.81 +0.6 2,781,281 + pinH b2648 0.81 -1.0 2,781,289 - pinH b2648 0.84 +0.0 2,781,289 - pinH b2648 0.84 +1.6 2,781,332 - +2.6 2,781,336 + +1.7 2,781,336 + -0.5 2,781,336 + -0.4 2,781,337 + -1.5 2,781,344 + -0.9 2,781,345 - +2.6 2,781,346 - +2.6 2,781,357 + -1.3 2,781,357 + -0.3 2,781,357 + -0.3 2,781,357 + -0.5 2,781,359 + -2.0 2,781,372 + -0.7 2,781,372 + -0.9 2,781,375 + -0.6 2,781,380 + -0.2 2,781,380 + +0.3 2,781,380 + -0.3 2,781,380 + -0.6 2,781,380 + -0.2 2,781,380 + -0.0 2,781,425 + -0.3 2,781,537 + +2.6 2,781,573 + -0.2 2,781,591 - +2.6 2,781,631 - +2.6 2,781,631 - +2.6 2,781,683 - -1.0 2,781,683 - +0.4 2,781,697 - +1.0 2,781,697 - +0.1 2,781,697 - +0.6 2,781,699 + +2.6 2,781,707 - -0.4 2,781,707 - +0.2 2,781,707 - +1.8 2,781,707 - +3.2 2,781,722 - +2.6 2,781,739 - +2.6 2,781,742 + +1.6 2,781,922 - ypjC b2650 0.19 +2.6 2,781,925 - ypjC b2650 0.19 +2.6 2,783,288 - +2.6 2,783,305 - +1.3 2,783,305 - +1.6 2,783,318 - +2.6 2,783,318 - +2.6 2,783,318 - +1.6 2,783,318 - +2.6 2,783,318 - +2.6 2,783,318 - +1.6 2,783,318 - +2.6 2,783,318 - +2.6 2,783,318 - +1.0 2,783,318 - +2.6 2,783,318 - +0.0 2,783,318 - +2.6 2,783,318 - +1.6 2,783,320 - +1.6 2,783,321 - +1.6 2,783,321 - +2.6 2,783,350 + +1.0 2,783,360 + +2.6 2,783,360 + +2.6 2,783,413 + +0.6 2,783,413 + +0.6 2,783,413 + +2.6 2,783,413 + +1.0 2,783,413 + +0.3 2,783,418 + +2.6 2,783,421 - +2.6 2,783,423 + -0.5 2,783,467 - +2.6 2,783,638 - +0.7 2,783,638 - -1.1 2,783,643 - -0.0 2,783,643 - +0.5 2,783,650 - +0.4 2,783,661 - +0.7 2,783,661 - -0.4 2,783,663 - +1.1 2,783,664 - +1.9 2,783,729 - +2.6 2,783,731 + -1.0 2,783,734 + +1.0 2,783,737 + +2.6 2,783,743 + +1.4 2,783,743 + -0.5 2,783,744 + -0.8 2,783,745 + +1.6 2,783,751 - +2.6 2,783,751 - +2.6 2,783,752 + +2.6 2,783,756 + +0.6 2,783,796 - ileY b2652 0.16 -0.5 2,783,821 - ileY b2652 0.49 +2.6 2,783,838 - ileY b2652 0.71 +2.6 2,783,843 - ileY b2652 0.78 +1.0 2,783,843 - ileY b2652 0.78 +2.6 2,783,843 - ileY b2652 0.78 +2.6 2,783,847 + ileY b2652 0.83 +2.6 2,783,847 + ileY b2652 0.83 +2.6 2,783,864 - +1.6 2,783,864 - +1.0 2,783,864 - +0.6 2,783,888 - -0.2 2,783,888 - -1.0 2,783,952 - +1.4 2,783,956 - -0.7 2,783,967 - +1.6 2,783,979 + +2.6 2,783,986 - +0.3 2,783,994 - +2.6 2,784,011 - +2.6 2,784,024 - +2.6
Or see this region's nucleotide sequence