Strain Fitness in Escherichia coli BW25113 around b1658

Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhP and ynhF are separated by 165 nucleotidesynhF and purR are separated by 298 nucleotidespurR and ydhB overlap by 4 nucleotides b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314 ydhP b4602: ynhF - hypothetical protein (RefSeq), at 1,735,480 to 1,735,569 ynhF b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893 purR b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822 ydhB Position (kb) 1735 1736 1737Strain fitness (log2 ratio) -1 0 1 2at 1734.906 kb on - strand, within ydhPat 1734.939 kb on - strand, within ydhPat 1735.214 kb on - strandat 1735.316 kb on + strandat 1735.660 kb on - strandat 1735.660 kb on - strandat 1735.783 kb on + strandat 1735.783 kb on + strandat 1735.791 kb on - strandat 1736.132 kb on + strand, within purRat 1736.147 kb on - strand, within purRat 1736.235 kb on - strand, within purRat 1736.243 kb on - strand, within purRat 1736.255 kb on + strand, within purRat 1736.255 kb on + strand, within purRat 1736.297 kb on + strand, within purRat 1736.319 kb on + strand, within purRat 1736.347 kb on - strand, within purRat 1736.506 kb on + strand, within purRat 1736.506 kb on + strand, within purRat 1736.688 kb on + strand, within purRat 1736.728 kb on + strand, within purRat 1736.728 kb on + strand, within purRat 1736.766 kb on - strand, within purRat 1736.820 kb on + strandat 1736.820 kb on - strandat 1736.843 kb on + strandat 1736.965 kb on + strandat 1737.011 kb on + strand, within ydhBat 1737.229 kb on - strand, within ydhBat 1737.332 kb on + strand, within ydhBat 1737.436 kb on - strandat 1737.689 kb on + strand, within ydhBat 1737.719 kb on + strand, within ydhB

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
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1,734,906 - ydhP b1657 0.65 +0.2
1,734,939 - ydhP b1657 0.68 -0.1
1,735,214 - -0.0
1,735,316 + +0.1
1,735,660 - -1.3
1,735,660 - -0.2
1,735,783 + -0.4
1,735,783 + +0.5
1,735,791 - -0.1
1,736,132 + purR b1658 0.26 +0.8
1,736,147 - purR b1658 0.27 +1.7
1,736,235 - purR b1658 0.36 -0.1
1,736,243 - purR b1658 0.37 +1.2
1,736,255 + purR b1658 0.38 +1.2
1,736,255 + purR b1658 0.38 +0.9
1,736,297 + purR b1658 0.42 +0.3
1,736,319 + purR b1658 0.44 +0.1
1,736,347 - purR b1658 0.47 +0.5
1,736,506 + purR b1658 0.62 -0.5
1,736,506 + purR b1658 0.62 -0.3
1,736,688 + purR b1658 0.80 +1.1
1,736,728 + purR b1658 0.84 +1.3
1,736,728 + purR b1658 0.84 +1.5
1,736,766 - purR b1658 0.88 -1.0
1,736,820 + -0.3
1,736,820 - +1.1
1,736,843 + +0.6
1,736,965 + -0.4
1,737,011 + ydhB b1659 0.13 +1.8
1,737,229 - ydhB b1659 0.36 +0.0
1,737,332 + ydhB b1659 0.47 -0.0
1,737,436 - +0.4
1,737,689 + ydhB b1659 0.86 +0.2
1,737,719 + ydhB b1659 0.89 +0.9

Or see this region's nucleotide sequence