Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sfmA and sfmC are separated by 219 nucleotides sfmC and sfmD are separated by 30 nucleotides
b0530: sfmA - putative fimbrial-like protein (VIMSS), at 557,435 to 557,977
sfmA
b0531: sfmC - pilin chaperone, periplasmic (NCBI), at 558,197 to 558,889
sfmC
b0532: sfmD - predicted outer membrane export usher protein (NCBI), at 558,920 to 561,523
sfmD
Position (kb) 558
559 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 557.226 kb on + strand at 557.584 kb on + strand, within sfmA at 557.588 kb on + strand, within sfmA at 557.707 kb on + strand, within sfmA at 557.767 kb on + strand, within sfmA at 557.786 kb on + strand, within sfmA at 557.824 kb on + strand, within sfmA at 557.838 kb on + strand, within sfmA at 557.845 kb on + strand, within sfmA at 557.856 kb on + strand, within sfmA at 557.870 kb on + strand, within sfmA at 557.900 kb on + strand, within sfmA at 557.900 kb on + strand, within sfmA at 557.902 kb on + strand, within sfmA at 557.902 kb on + strand, within sfmA at 557.914 kb on + strand, within sfmA at 557.916 kb on + strand, within sfmA at 557.916 kb on + strand, within sfmA at 557.925 kb on + strand at 557.925 kb on + strand at 557.928 kb on + strand at 557.933 kb on + strand at 557.938 kb on + strand at 557.940 kb on + strand at 557.957 kb on + strand at 557.957 kb on + strand at 558.080 kb on + strand at 558.084 kb on + strand at 558.274 kb on - strand, within sfmC at 558.320 kb on - strand, within sfmC at 558.320 kb on - strand, within sfmC at 558.332 kb on - strand, within sfmC at 558.334 kb on + strand, within sfmC at 558.383 kb on + strand, within sfmC at 558.404 kb on - strand, within sfmC at 558.425 kb on - strand, within sfmC at 558.439 kb on - strand, within sfmC at 558.480 kb on - strand, within sfmC at 558.480 kb on - strand, within sfmC at 558.480 kb on - strand, within sfmC at 558.488 kb on - strand, within sfmC at 558.501 kb on - strand, within sfmC at 558.517 kb on + strand, within sfmC at 558.539 kb on + strand, within sfmC at 558.556 kb on - strand, within sfmC at 558.650 kb on - strand, within sfmC at 558.659 kb on - strand, within sfmC at 558.693 kb on - strand, within sfmC at 558.695 kb on - strand, within sfmC at 558.695 kb on - strand, within sfmC at 558.695 kb on - strand, within sfmC at 558.719 kb on - strand, within sfmC at 558.719 kb on - strand, within sfmC at 558.722 kb on + strand, within sfmC at 558.722 kb on + strand, within sfmC at 558.733 kb on - strand, within sfmC at 558.735 kb on - strand, within sfmC at 558.740 kb on - strand, within sfmC at 558.740 kb on - strand, within sfmC at 558.755 kb on - strand, within sfmC at 558.763 kb on + strand, within sfmC at 558.763 kb on + strand, within sfmC at 558.763 kb on + strand, within sfmC at 558.771 kb on - strand, within sfmC at 558.771 kb on - strand, within sfmC at 558.771 kb on - strand, within sfmC at 558.774 kb on + strand, within sfmC at 558.775 kb on + strand, within sfmC at 558.810 kb on - strand, within sfmC at 558.812 kb on + strand, within sfmC at 558.812 kb on + strand, within sfmC at 558.812 kb on + strand, within sfmC at 558.812 kb on + strand, within sfmC at 558.820 kb on - strand at 558.852 kb on + strand at 558.852 kb on + strand at 558.855 kb on + strand at 558.906 kb on + strand at 558.939 kb on - strand at 558.955 kb on + strand at 558.960 kb on - strand at 559.010 kb on + strand at 559.010 kb on + strand at 559.102 kb on + strand at 559.110 kb on - strand at 559.110 kb on - strand at 559.110 kb on - strand at 559.110 kb on - strand at 559.145 kb on + strand at 559.146 kb on - strand at 559.178 kb on + strand at 559.211 kb on + strand, within sfmD at 559.227 kb on + strand, within sfmD at 559.227 kb on + strand, within sfmD at 559.261 kb on + strand, within sfmD at 559.384 kb on + strand, within sfmD at 559.414 kb on + strand, within sfmD at 559.416 kb on + strand, within sfmD at 559.416 kb on + strand, within sfmD at 559.420 kb on + strand, within sfmD at 559.420 kb on + strand, within sfmD at 559.420 kb on + strand, within sfmD at 559.422 kb on - strand, within sfmD at 559.425 kb on + strand, within sfmD at 559.433 kb on + strand, within sfmD at 559.439 kb on + strand, within sfmD at 559.454 kb on + strand, within sfmD at 559.569 kb on - strand, within sfmD at 559.659 kb on - strand, within sfmD at 559.664 kb on - strand, within sfmD at 559.777 kb on - strand, within sfmD at 559.784 kb on - strand, within sfmD at 559.792 kb on - strand, within sfmD at 559.794 kb on - strand, within sfmD at 559.797 kb on + strand, within sfmD at 559.798 kb on + strand, within sfmD at 559.799 kb on - strand, within sfmD at 559.801 kb on + strand, within sfmD at 559.805 kb on - strand, within sfmD at 559.805 kb on - strand, within sfmD at 559.805 kb on - strand, within sfmD at 559.812 kb on + strand, within sfmD
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 557,226 + +0.4 557,584 + sfmA b0530 0.27 +1.3 557,588 + sfmA b0530 0.28 +2.2 557,707 + sfmA b0530 0.50 +2.2 557,767 + sfmA b0530 0.61 +2.2 557,786 + sfmA b0530 0.65 +2.2 557,824 + sfmA b0530 0.72 +2.2 557,838 + sfmA b0530 0.74 +2.2 557,845 + sfmA b0530 0.76 +2.2 557,856 + sfmA b0530 0.78 -0.1 557,870 + sfmA b0530 0.80 +1.7 557,900 + sfmA b0530 0.86 +0.0 557,900 + sfmA b0530 0.86 +1.9 557,902 + sfmA b0530 0.86 +0.6 557,902 + sfmA b0530 0.86 +1.4 557,914 + sfmA b0530 0.88 +0.6 557,916 + sfmA b0530 0.89 +0.7 557,916 + sfmA b0530 0.89 -0.6 557,925 + +0.9 557,925 + -0.8 557,928 + +0.0 557,933 + +0.5 557,938 + -0.4 557,940 + -0.0 557,957 + +3.2 557,957 + +0.7 558,080 + +2.2 558,084 + +2.2 558,274 - sfmC b0531 0.11 +2.2 558,320 - sfmC b0531 0.18 +2.2 558,320 - sfmC b0531 0.18 +1.2 558,332 - sfmC b0531 0.19 +2.2 558,334 + sfmC b0531 0.20 +2.2 558,383 + sfmC b0531 0.27 +0.7 558,404 - sfmC b0531 0.30 +2.2 558,425 - sfmC b0531 0.33 +2.2 558,439 - sfmC b0531 0.35 +2.2 558,480 - sfmC b0531 0.41 +1.0 558,480 - sfmC b0531 0.41 +1.4 558,480 - sfmC b0531 0.41 -0.2 558,488 - sfmC b0531 0.42 -0.8 558,501 - sfmC b0531 0.44 -2.0 558,517 + sfmC b0531 0.46 +2.2 558,539 + sfmC b0531 0.49 +2.2 558,556 - sfmC b0531 0.52 +0.6 558,650 - sfmC b0531 0.65 +0.6 558,659 - sfmC b0531 0.67 -0.5 558,693 - sfmC b0531 0.72 +0.3 558,695 - sfmC b0531 0.72 +2.2 558,695 - sfmC b0531 0.72 +2.9 558,695 - sfmC b0531 0.72 +0.2 558,719 - sfmC b0531 0.75 +0.2 558,719 - sfmC b0531 0.75 +1.2 558,722 + sfmC b0531 0.76 -0.4 558,722 + sfmC b0531 0.76 +0.6 558,733 - sfmC b0531 0.77 +0.8 558,735 - sfmC b0531 0.78 +0.4 558,740 - sfmC b0531 0.78 +0.4 558,740 - sfmC b0531 0.78 -1.6 558,755 - sfmC b0531 0.81 +2.7 558,763 + sfmC b0531 0.82 +0.6 558,763 + sfmC b0531 0.82 +2.2 558,763 + sfmC b0531 0.82 +2.2 558,771 - sfmC b0531 0.83 +2.2 558,771 - sfmC b0531 0.83 +2.2 558,771 - sfmC b0531 0.83 +2.2 558,774 + sfmC b0531 0.83 +0.2 558,775 + sfmC b0531 0.83 +2.2 558,810 - sfmC b0531 0.88 -2.6 558,812 + sfmC b0531 0.89 +2.2 558,812 + sfmC b0531 0.89 +2.2 558,812 + sfmC b0531 0.89 +2.2 558,812 + sfmC b0531 0.89 +0.6 558,820 - -0.1 558,852 + +2.2 558,852 + +0.4 558,855 + +2.2 558,906 + +2.2 558,939 - +2.2 558,955 + +2.2 558,960 - +2.2 559,010 + +2.2 559,010 + +2.2 559,102 + +1.2 559,110 - +1.2 559,110 - +0.2 559,110 - -0.3 559,110 - +0.7 559,145 + +2.2 559,146 - +2.2 559,178 + +2.2 559,211 + sfmD b0532 0.11 +2.2 559,227 + sfmD b0532 0.12 +2.2 559,227 + sfmD b0532 0.12 +2.2 559,261 + sfmD b0532 0.13 +2.2 559,384 + sfmD b0532 0.18 +2.2 559,414 + sfmD b0532 0.19 +1.2 559,416 + sfmD b0532 0.19 +2.2 559,416 + sfmD b0532 0.19 +2.2 559,420 + sfmD b0532 0.19 +0.6 559,420 + sfmD b0532 0.19 +0.0 559,420 + sfmD b0532 0.19 +0.9 559,422 - sfmD b0532 0.19 +2.2 559,425 + sfmD b0532 0.19 +1.2 559,433 + sfmD b0532 0.20 -0.1 559,439 + sfmD b0532 0.20 +2.2 559,454 + sfmD b0532 0.21 +0.2 559,569 - sfmD b0532 0.25 +2.2 559,659 - sfmD b0532 0.28 -0.8 559,664 - sfmD b0532 0.29 +2.2 559,777 - sfmD b0532 0.33 +1.2 559,784 - sfmD b0532 0.33 +2.2 559,792 - sfmD b0532 0.33 +0.6 559,794 - sfmD b0532 0.34 +2.2 559,797 + sfmD b0532 0.34 +2.2 559,798 + sfmD b0532 0.34 +2.2 559,799 - sfmD b0532 0.34 +2.2 559,801 + sfmD b0532 0.34 -0.1 559,805 - sfmD b0532 0.34 +2.2 559,805 - sfmD b0532 0.34 +2.2 559,805 - sfmD b0532 0.34 +0.1 559,812 + sfmD b0532 0.34 +2.2
Or see this region's nucleotide sequence