Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfB and hyfC are separated by 10 nucleotides hyfC and hyfD are separated by 16 nucleotides hyfD and hyfE are separated by 11 nucleotides hyfE and hyfF are separated by 4 nucleotides
b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858
hyfB
b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816
hyfC
b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272
hyfD
b2485: hyfE - hydrogenase 4, membrane subunit (NCBI), at 2,604,284 to 2,604,934
hyfE
b2486: hyfF - NADH dehydrogenase subunit N (NCBI), at 2,604,939 to 2,606,519
hyfF
Position (kb)
2602
2603
2604
2605 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2601.925 kb on + strand at 2601.925 kb on + strand at 2601.988 kb on - strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.150 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.325 kb on + strand, within hyfC at 2602.350 kb on + strand, within hyfC at 2602.379 kb on + strand, within hyfC at 2602.382 kb on - strand, within hyfC at 2602.432 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.813 kb on + strand at 2602.813 kb on + strand at 2603.063 kb on - strand, within hyfD at 2603.095 kb on - strand, within hyfD at 2603.151 kb on + strand, within hyfD at 2603.151 kb on + strand, within hyfD at 2603.293 kb on + strand, within hyfD at 2603.301 kb on - strand, within hyfD at 2603.328 kb on - strand, within hyfD at 2603.369 kb on + strand, within hyfD at 2603.430 kb on + strand, within hyfD at 2603.464 kb on - strand, within hyfD at 2603.464 kb on - strand, within hyfD at 2603.477 kb on - strand, within hyfD at 2603.547 kb on - strand, within hyfD at 2603.570 kb on - strand, within hyfD at 2603.574 kb on + strand, within hyfD at 2603.574 kb on + strand, within hyfD at 2603.613 kb on + strand, within hyfD at 2603.716 kb on + strand, within hyfD at 2603.726 kb on + strand, within hyfD at 2603.726 kb on + strand, within hyfD at 2603.783 kb on + strand, within hyfD at 2603.882 kb on + strand, within hyfD at 2603.882 kb on + strand, within hyfD at 2603.933 kb on - strand, within hyfD at 2603.933 kb on - strand, within hyfD at 2604.064 kb on - strand, within hyfD at 2604.064 kb on - strand, within hyfD at 2604.216 kb on + strand at 2604.216 kb on + strand at 2604.284 kb on - strand at 2604.284 kb on - strand at 2604.340 kb on + strand at 2604.488 kb on + strand, within hyfE at 2604.488 kb on + strand, within hyfE at 2604.513 kb on - strand, within hyfE at 2604.598 kb on - strand, within hyfE at 2604.626 kb on + strand, within hyfE at 2604.700 kb on + strand, within hyfE at 2604.708 kb on - strand, within hyfE at 2604.708 kb on - strand, within hyfE at 2604.828 kb on + strand, within hyfE at 2604.862 kb on - strand, within hyfE at 2604.882 kb on + strand at 2604.882 kb on + strand at 2605.055 kb on - strand at 2605.065 kb on - strand at 2605.065 kb on - strand at 2605.071 kb on - strand at 2605.140 kb on - strand, within hyfF at 2605.210 kb on + strand, within hyfF at 2605.256 kb on + strand, within hyfF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 2,601,925 + -1.1 2,601,925 + +0.2 2,601,988 - hyfC b2483 0.13 -0.2 2,602,127 + hyfC b2483 0.27 +0.3 2,602,127 + hyfC b2483 0.27 +0.2 2,602,150 - hyfC b2483 0.30 +0.4 2,602,199 - hyfC b2483 0.35 +0.4 2,602,199 - hyfC b2483 0.35 +0.1 2,602,325 + hyfC b2483 0.48 -0.1 2,602,350 + hyfC b2483 0.51 -0.4 2,602,379 + hyfC b2483 0.54 +0.4 2,602,382 - hyfC b2483 0.54 -0.4 2,602,432 - hyfC b2483 0.59 -0.9 2,602,620 - hyfC b2483 0.79 +0.1 2,602,620 - hyfC b2483 0.79 +0.4 2,602,813 + -0.1 2,602,813 + +1.0 2,603,063 - hyfD b2484 0.16 -1.0 2,603,095 - hyfD b2484 0.18 -0.1 2,603,151 + hyfD b2484 0.22 -0.8 2,603,151 + hyfD b2484 0.22 +0.1 2,603,293 + hyfD b2484 0.32 +1.9 2,603,301 - hyfD b2484 0.33 +0.3 2,603,328 - hyfD b2484 0.34 +0.0 2,603,369 + hyfD b2484 0.37 -1.0 2,603,430 + hyfD b2484 0.41 -0.3 2,603,464 - hyfD b2484 0.44 +0.7 2,603,464 - hyfD b2484 0.44 -0.4 2,603,477 - hyfD b2484 0.45 -0.6 2,603,547 - hyfD b2484 0.50 +0.9 2,603,570 - hyfD b2484 0.51 -0.6 2,603,574 + hyfD b2484 0.51 +0.4 2,603,574 + hyfD b2484 0.51 -0.1 2,603,613 + hyfD b2484 0.54 +0.1 2,603,716 + hyfD b2484 0.61 -0.0 2,603,726 + hyfD b2484 0.62 +1.0 2,603,726 + hyfD b2484 0.62 +0.1 2,603,783 + hyfD b2484 0.66 +0.7 2,603,882 + hyfD b2484 0.73 -0.9 2,603,882 + hyfD b2484 0.73 -0.1 2,603,933 - hyfD b2484 0.76 -1.8 2,603,933 - hyfD b2484 0.76 -0.4 2,604,064 - hyfD b2484 0.85 -0.5 2,604,064 - hyfD b2484 0.85 -0.7 2,604,216 + -0.2 2,604,216 + -0.8 2,604,284 - +0.8 2,604,284 - -0.1 2,604,340 + +0.2 2,604,488 + hyfE b2485 0.31 +0.8 2,604,488 + hyfE b2485 0.31 -2.2 2,604,513 - hyfE b2485 0.35 -0.6 2,604,598 - hyfE b2485 0.48 -1.3 2,604,626 + hyfE b2485 0.53 +0.5 2,604,700 + hyfE b2485 0.64 +0.8 2,604,708 - hyfE b2485 0.65 -0.3 2,604,708 - hyfE b2485 0.65 +0.0 2,604,828 + hyfE b2485 0.84 -1.7 2,604,862 - hyfE b2485 0.89 +0.1 2,604,882 + -0.7 2,604,882 + +0.2 2,605,055 - -0.7 2,605,065 - -0.3 2,605,065 - -0.4 2,605,071 - +0.1 2,605,140 - hyfF b2486 0.13 +1.8 2,605,210 + hyfF b2486 0.17 +0.1 2,605,256 + hyfF b2486 0.20 -0.5
Or see this region's nucleotide sequence