Strain Fitness in Escherichia coli BW25113 around b1860

Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntznuC and znuB overlap by 4 nucleotidesznuB and ruvB are separated by 146 nucleotidesruvB and ruvA are separated by 8 nucleotidesruvA and yobI are separated by 138 nucleotidesyobI and yebB are separated by 70 nucleotides b1858: znuC - high-affinity zinc transporter ATPase (NCBI), at 1,940,686 to 1,941,441 znuC b1859: znuB - high-affinity zinc transporter membrane component (NCBI), at 1,941,438 to 1,942,223 znuB b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380 ruvB b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000 ruvA b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204 yobI b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877 yebB Position (kb) 1942 1943 1944Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1941.385 kb on - strandat 1941.492 kb on - strandat 1941.587 kb on + strand, within znuBat 1941.587 kb on + strand, within znuBat 1941.589 kb on + strand, within znuBat 1941.662 kb on + strand, within znuBat 1941.662 kb on + strandat 1941.704 kb on + strand, within znuBat 1941.712 kb on - strandat 1941.714 kb on + strand, within znuBat 1941.749 kb on + strand, within znuBat 1941.914 kb on + strand, within znuBat 1941.914 kb on + strand, within znuBat 1941.978 kb on + strand, within znuBat 1942.038 kb on + strand, within znuBat 1942.038 kb on + strand, within znuBat 1942.094 kb on + strand, within znuBat 1942.094 kb on + strand, within znuBat 1942.156 kb on - strandat 1942.156 kb on - strandat 1942.184 kb on + strandat 1942.295 kb on + strandat 1942.295 kb on + strandat 1942.423 kb on + strandat 1942.445 kb on - strandat 1942.446 kb on - strandat 1942.806 kb on + strand, within ruvBat 1942.985 kb on + strand, within ruvBat 1943.892 kb on + strand, within ruvAat 1943.919 kb on - strand, within ruvAat 1943.934 kb on - strand, within ruvAat 1943.990 kb on - strandat 1943.996 kb on + strandat 1943.996 kb on + strandat 1944.021 kb on + strandat 1944.102 kb on + strandat 1944.102 kb on + strandat 1944.137 kb on + strandat 1944.333 kb on - strandat 1944.333 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
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1,941,385 - +0.1
1,941,492 - -1.6
1,941,587 + znuB b1859 0.19 -0.2
1,941,587 + znuB b1859 0.19 +0.7
1,941,589 + znuB b1859 0.19 -0.1
1,941,662 + znuB b1859 0.28 +0.9
1,941,662 + +1.9
1,941,704 + znuB b1859 0.34 +0.8
1,941,712 - +2.0
1,941,714 + znuB b1859 0.35 -1.2
1,941,749 + znuB b1859 0.40 -0.2
1,941,914 + znuB b1859 0.61 -0.1
1,941,914 + znuB b1859 0.61 -0.7
1,941,978 + znuB b1859 0.69 +0.1
1,942,038 + znuB b1859 0.76 -1.0
1,942,038 + znuB b1859 0.76 +0.3
1,942,094 + znuB b1859 0.83 -0.8
1,942,094 + znuB b1859 0.83 -2.3
1,942,156 - +3.8
1,942,156 - +1.8
1,942,184 + -0.9
1,942,295 + -1.7
1,942,295 + -0.6
1,942,423 + +0.4
1,942,445 - +1.8
1,942,446 - +1.8
1,942,806 + ruvB b1860 0.43 +2.8
1,942,985 + ruvB b1860 0.61 +2.8
1,943,892 + ruvA b1861 0.82 +2.8
1,943,919 - ruvA b1861 0.87 +1.8
1,943,934 - ruvA b1861 0.89 +1.2
1,943,990 - +1.8
1,943,996 + +2.8
1,943,996 + +2.8
1,944,021 + -1.3
1,944,102 + -0.8
1,944,102 + +1.3
1,944,137 + +1.0
1,944,333 - -0.4
1,944,333 - -0.6

Or see this region's nucleotide sequence