Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt znuC and znuB overlap by 4 nucleotides znuB and ruvB are separated by 146 nucleotides ruvB and ruvA are separated by 8 nucleotides ruvA and yobI are separated by 138 nucleotides yobI and yebB are separated by 70 nucleotides
b1858: znuC - high-affinity zinc transporter ATPase (NCBI), at 1,940,686 to 1,941,441
znuC
b1859: znuB - high-affinity zinc transporter membrane component (NCBI), at 1,941,438 to 1,942,223
znuB
b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380
ruvB
b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000
ruvA
b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204
yobI
b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877
yebB
Position (kb)
1942
1943
1944 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1941.385 kb on - strand at 1941.492 kb on - strand at 1941.587 kb on + strand, within znuB at 1941.587 kb on + strand, within znuB at 1941.589 kb on + strand, within znuB at 1941.662 kb on + strand, within znuB at 1941.662 kb on + strand at 1941.704 kb on + strand, within znuB at 1941.712 kb on - strand at 1941.714 kb on + strand, within znuB at 1941.749 kb on + strand, within znuB at 1941.914 kb on + strand, within znuB at 1941.914 kb on + strand, within znuB at 1941.978 kb on + strand, within znuB at 1942.038 kb on + strand, within znuB at 1942.038 kb on + strand, within znuB at 1942.094 kb on + strand, within znuB at 1942.094 kb on + strand, within znuB at 1942.156 kb on - strand at 1942.156 kb on - strand at 1942.184 kb on + strand at 1942.295 kb on + strand at 1942.295 kb on + strand at 1942.423 kb on + strand at 1942.445 kb on - strand at 1942.446 kb on - strand at 1942.806 kb on + strand, within ruvB at 1942.985 kb on + strand, within ruvB at 1943.892 kb on + strand, within ruvA at 1943.919 kb on - strand, within ruvA at 1943.934 kb on - strand, within ruvA at 1943.990 kb on - strand at 1943.996 kb on + strand at 1943.996 kb on + strand at 1944.021 kb on + strand at 1944.102 kb on + strand at 1944.102 kb on + strand at 1944.137 kb on + strand at 1944.333 kb on - strand at 1944.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 1,941,385 - +0.1 1,941,492 - -1.6 1,941,587 + znuB b1859 0.19 -0.2 1,941,587 + znuB b1859 0.19 +0.7 1,941,589 + znuB b1859 0.19 -0.1 1,941,662 + znuB b1859 0.28 +0.9 1,941,662 + +1.9 1,941,704 + znuB b1859 0.34 +0.8 1,941,712 - +2.0 1,941,714 + znuB b1859 0.35 -1.2 1,941,749 + znuB b1859 0.40 -0.2 1,941,914 + znuB b1859 0.61 -0.1 1,941,914 + znuB b1859 0.61 -0.7 1,941,978 + znuB b1859 0.69 +0.1 1,942,038 + znuB b1859 0.76 -1.0 1,942,038 + znuB b1859 0.76 +0.3 1,942,094 + znuB b1859 0.83 -0.8 1,942,094 + znuB b1859 0.83 -2.3 1,942,156 - +3.8 1,942,156 - +1.8 1,942,184 + -0.9 1,942,295 + -1.7 1,942,295 + -0.6 1,942,423 + +0.4 1,942,445 - +1.8 1,942,446 - +1.8 1,942,806 + ruvB b1860 0.43 +2.8 1,942,985 + ruvB b1860 0.61 +2.8 1,943,892 + ruvA b1861 0.82 +2.8 1,943,919 - ruvA b1861 0.87 +1.8 1,943,934 - ruvA b1861 0.89 +1.2 1,943,990 - +1.8 1,943,996 + +2.8 1,943,996 + +2.8 1,944,021 + -1.3 1,944,102 + -0.8 1,944,102 + +1.3 1,944,137 + +1.0 1,944,333 - -0.4 1,944,333 - -0.6
Or see this region's nucleotide sequence