Strain Fitness in Escherichia coli BW25113 around b1600

Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydgD and mdtI are separated by 38 nucleotidesmdtI and mdtJ overlap by 14 nucleotidesmdtJ and ydgG are separated by 411 nucleotides b1598: ydgD - predicted peptidase (NCBI), at 1,669,984 to 1,670,805 ydgD b1599: mdtI - multidrug efflux system transporter (NCBI), at 1,670,844 to 1,671,173 mdtI b1600: mdtJ - multidrug efflux system transporter (NCBI), at 1,671,160 to 1,671,525 mdtJ b1601: ydgG - predicted inner membrane protein (NCBI), at 1,671,937 to 1,672,971 ydgG Position (kb) 1671 1672Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1670.161 kb on - strand, within ydgDat 1670.248 kb on + strand, within ydgDat 1670.337 kb on - strand, within ydgDat 1670.575 kb on + strand, within ydgDat 1670.673 kb on - strand, within ydgDat 1671.156 kb on - strandat 1671.156 kb on - strandat 1671.165 kb on - strandat 1671.182 kb on - strandat 1671.186 kb on + strandat 1671.251 kb on - strand, within mdtJat 1671.253 kb on - strand, within mdtJat 1671.346 kb on + strand, within mdtJat 1671.348 kb on - strand, within mdtJat 1671.348 kb on - strand, within mdtJat 1671.354 kb on - strand, within mdtJat 1671.354 kb on - strand, within mdtJat 1671.355 kb on - strand, within mdtJat 1671.449 kb on - strand, within mdtJat 1671.466 kb on - strand, within mdtJat 1671.466 kb on - strand, within mdtJat 1671.474 kb on - strand, within mdtJat 1671.525 kb on - strandat 1671.595 kb on - strandat 1671.615 kb on - strandat 1671.653 kb on - strandat 1671.653 kb on - strandat 1671.653 kb on - strandat 1671.653 kb on - strandat 1671.665 kb on + strandat 1671.761 kb on - strandat 1671.761 kb on - strandat 1671.761 kb on - strandat 1671.800 kb on - strandat 1671.800 kb on - strandat 1671.927 kb on + strandat 1671.935 kb on + strandat 1671.941 kb on - strandat 1671.943 kb on - strandat 1672.027 kb on + strandat 1672.082 kb on - strand, within ydgGat 1672.082 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.102 kb on - strand, within ydgGat 1672.106 kb on + strand, within ydgGat 1672.151 kb on - strand, within ydgGat 1672.157 kb on - strand, within ydgGat 1672.291 kb on - strand, within ydgGat 1672.365 kb on + strand, within ydgGat 1672.365 kb on + strand, within ydgGat 1672.413 kb on + strand, within ydgGat 1672.481 kb on + strand, within ydgG

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
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1,670,161 - ydgD b1598 0.22 -0.1
1,670,248 + ydgD b1598 0.32 -0.1
1,670,337 - ydgD b1598 0.43 -0.4
1,670,575 + ydgD b1598 0.72 -0.6
1,670,673 - ydgD b1598 0.84 -0.5
1,671,156 - -0.5
1,671,156 - -0.2
1,671,165 - +0.1
1,671,182 - -0.9
1,671,186 + +1.2
1,671,251 - mdtJ b1600 0.25 +1.1
1,671,253 - mdtJ b1600 0.25 +1.3
1,671,346 + mdtJ b1600 0.51 +2.7
1,671,348 - mdtJ b1600 0.51 +1.1
1,671,348 - mdtJ b1600 0.51 +1.1
1,671,354 - mdtJ b1600 0.53 +0.1
1,671,354 - mdtJ b1600 0.53 -0.1
1,671,355 - mdtJ b1600 0.53 -0.9
1,671,449 - mdtJ b1600 0.79 +3.1
1,671,466 - mdtJ b1600 0.84 +4.7
1,671,466 - mdtJ b1600 0.84 +0.1
1,671,474 - mdtJ b1600 0.86 +1.7
1,671,525 - +2.7
1,671,595 - +0.4
1,671,615 - -0.1
1,671,653 - +0.7
1,671,653 - +0.4
1,671,653 - +0.3
1,671,653 - +0.1
1,671,665 + +2.7
1,671,761 - -0.0
1,671,761 - -0.1
1,671,761 - -0.2
1,671,800 - -0.5
1,671,800 - +1.0
1,671,927 + +2.7
1,671,935 + +2.7
1,671,941 - -2.5
1,671,943 - +2.7
1,672,027 + -0.0
1,672,082 - ydgG b1601 0.14 +0.2
1,672,082 - ydgG b1601 0.14 -1.3
1,672,095 - ydgG b1601 0.15 +0.6
1,672,095 - ydgG b1601 0.15 +2.3
1,672,095 - ydgG b1601 0.15 -1.1
1,672,102 - ydgG b1601 0.16 +0.8
1,672,106 + ydgG b1601 0.16 -1.2
1,672,151 - ydgG b1601 0.21 -0.0
1,672,157 - ydgG b1601 0.21 +0.9
1,672,291 - ydgG b1601 0.34 -0.4
1,672,365 + ydgG b1601 0.41 -0.4
1,672,365 + ydgG b1601 0.41 +0.4
1,672,413 + ydgG b1601 0.46 -0.6
1,672,481 + ydgG b1601 0.53 +0.9

Or see this region's nucleotide sequence