Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfO and ydfO are separated by 57 nucleotides ydfO and b1550 are separated by 151 nucleotides b1550 and b1551 are separated by 171 nucleotides b1551 and cspI are separated by 345 nucleotides cspI and ydfP are separated by 362 nucleotides
b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013
ynfO
b1549: ydfO - orf, hypothetical protein (VIMSS), at 1,635,071 to 1,635,481
ydfO
b1550: b1550 - orf, hypothetical protein (VIMSS), at 1,635,633 to 1,635,806
b1550
b1551: b1551 - orf, hypothetical protein (VIMSS), at 1,635,978 to 1,636,133
b1551
b1552: cspI - Qin prophage; cold shock protein (NCBI), at 1,636,479 to 1,636,691
cspI
b1553: ydfP - Qin prophage; conserved protein (NCBI), at 1,637,054 to 1,637,551
ydfP
b4689: rzoQ - hypothetical protein (RefSeq), at 1,637,104 to 1,637,358
rzoQ
Position (kb)
1635
1636
1637 Strain fitness (log2 ratio)
-1
0
1
2 at 1635.711 kb on - strand, within b1550 at 1635.711 kb on - strand, within b1550 at 1635.823 kb on - strand at 1635.823 kb on - strand at 1635.847 kb on - strand at 1635.847 kb on - strand at 1636.078 kb on - strand, within b1551 at 1636.097 kb on - strand, within b1551 at 1636.212 kb on + strand at 1636.353 kb on - strand at 1636.354 kb on + strand at 1636.421 kb on - strand at 1636.426 kb on - strand at 1636.428 kb on - strand at 1636.434 kb on - strand at 1636.434 kb on - strand at 1636.499 kb on - strand at 1636.500 kb on - strand at 1637.122 kb on - strand at 1637.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 1,635,711 - b1550 b1550 0.45 +0.4 1,635,711 - b1550 b1550 0.45 -0.6 1,635,823 - +0.1 1,635,823 - +0.1 1,635,847 - -0.2 1,635,847 - +0.0 1,636,078 - b1551 b1551 0.64 +2.6 1,636,097 - b1551 b1551 0.76 +2.6 1,636,212 + +2.6 1,636,353 - +0.8 1,636,354 + +2.6 1,636,421 - +2.6 1,636,426 - +1.5 1,636,428 - +0.0 1,636,434 - +1.6 1,636,434 - +2.6 1,636,499 - +2.6 1,636,500 - +2.6 1,637,122 - -0.6 1,637,122 - +0.6
Or see this region's nucleotide sequence