Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides tolA and tolB are separated by 132 nucleotides
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
b0738: tolR - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 775,072 to 775,500
tolR
b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830
tolA
b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255
tolB
Position (kb) 775
776
777 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 774.571 kb on + strand, within tolQ at 774.649 kb on + strand, within tolQ at 774.668 kb on - strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.809 kb on - strand, within tolQ at 774.813 kb on - strand, within tolQ at 774.818 kb on - strand, within tolQ at 774.839 kb on + strand, within tolQ at 774.905 kb on + strand, within tolQ at 774.956 kb on + strand, within tolQ at 775.063 kb on + strand at 775.063 kb on + strand at 775.070 kb on - strand at 775.071 kb on - strand at 775.071 kb on - strand at 775.091 kb on - strand at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.146 kb on + strand, within tolR at 775.152 kb on - strand, within tolR at 775.194 kb on - strand, within tolR at 775.258 kb on + strand, within tolR at 775.265 kb on + strand, within tolR at 775.275 kb on + strand, within tolR at 775.280 kb on + strand, within tolR at 775.288 kb on - strand, within tolR at 775.288 kb on - strand, within tolR at 775.298 kb on - strand, within tolR at 775.333 kb on + strand, within tolR at 775.333 kb on + strand, within tolR at 775.357 kb on - strand, within tolR at 775.526 kb on - strand at 775.533 kb on + strand at 775.565 kb on + strand at 775.628 kb on + strand at 775.651 kb on + strand at 775.659 kb on - strand at 775.672 kb on + strand at 775.748 kb on + strand, within tolA at 775.753 kb on + strand, within tolA at 775.840 kb on + strand, within tolA at 775.930 kb on - strand, within tolA at 775.932 kb on + strand, within tolA at 775.947 kb on + strand, within tolA at 776.098 kb on - strand, within tolA at 776.124 kb on + strand, within tolA at 776.146 kb on + strand, within tolA at 776.265 kb on - strand, within tolA at 776.265 kb on - strand, within tolA at 776.464 kb on + strand, within tolA at 776.475 kb on - strand, within tolA at 776.601 kb on - strand, within tolA at 776.625 kb on - strand, within tolA at 776.640 kb on - strand, within tolA at 776.640 kb on - strand, within tolA at 776.657 kb on - strand, within tolA at 776.713 kb on - strand at 776.725 kb on + strand at 776.779 kb on - strand at 776.943 kb on + strand at 776.968 kb on - strand at 776.994 kb on + strand at 776.994 kb on + strand at 777.012 kb on + strand at 777.014 kb on + strand at 777.135 kb on + strand, within tolB at 777.146 kb on + strand, within tolB at 777.260 kb on + strand, within tolB at 777.278 kb on - strand, within tolB at 777.299 kb on - strand, within tolB at 777.303 kb on - strand, within tolB at 777.393 kb on - strand, within tolB at 777.398 kb on - strand, within tolB at 777.444 kb on - strand, within tolB at 777.513 kb on + strand, within tolB at 777.522 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.692 kb on + strand, within tolB at 777.700 kb on - strand, within tolB at 777.720 kb on - strand, within tolB at 777.770 kb on - strand, within tolB at 777.792 kb on - strand, within tolB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 774,571 + tolQ b0737 0.28 +4.4 774,649 + tolQ b0737 0.39 +0.8 774,668 - tolQ b0737 0.42 +2.4 774,723 + tolQ b0737 0.50 +0.4 774,723 + tolQ b0737 0.50 +2.4 774,809 - tolQ b0737 0.62 +2.4 774,813 - tolQ b0737 0.63 +2.4 774,818 - tolQ b0737 0.64 +2.4 774,839 + tolQ b0737 0.67 +2.4 774,905 + tolQ b0737 0.76 +1.4 774,956 + tolQ b0737 0.84 +2.4 775,063 + -0.9 775,063 + -2.0 775,070 - -0.0 775,071 - -2.4 775,071 - -2.3 775,091 - -0.1 775,121 + tolR b0738 0.11 +2.4 775,121 + tolR b0738 0.11 +2.4 775,121 + tolR b0738 0.11 +2.4 775,146 + tolR b0738 0.17 +2.4 775,152 - tolR b0738 0.19 +2.4 775,194 - tolR b0738 0.28 +2.4 775,258 + tolR b0738 0.43 +1.4 775,265 + tolR b0738 0.45 +2.4 775,275 + tolR b0738 0.47 +2.4 775,280 + tolR b0738 0.48 +1.4 775,288 - tolR b0738 0.50 +2.4 775,288 - tolR b0738 0.50 +2.4 775,298 - tolR b0738 0.53 +2.4 775,333 + tolR b0738 0.61 +1.4 775,333 + tolR b0738 0.61 +2.4 775,357 - tolR b0738 0.66 +1.4 775,526 - +0.0 775,533 + +0.6 775,565 + +0.9 775,628 + +2.4 775,651 + +2.4 775,659 - +2.4 775,672 + +1.4 775,748 + tolA b0739 0.14 +0.4 775,753 + tolA b0739 0.15 +2.4 775,840 + tolA b0739 0.22 +2.4 775,930 - tolA b0739 0.29 +2.4 775,932 + tolA b0739 0.29 +0.4 775,947 + tolA b0739 0.30 +2.4 776,098 - tolA b0739 0.42 +2.4 776,124 + tolA b0739 0.44 +1.4 776,146 + tolA b0739 0.46 +2.4 776,265 - tolA b0739 0.55 +2.4 776,265 - tolA b0739 0.55 +1.4 776,464 + tolA b0739 0.71 +2.4 776,475 - tolA b0739 0.72 +1.4 776,601 - tolA b0739 0.82 +2.4 776,625 - tolA b0739 0.84 +1.4 776,640 - tolA b0739 0.85 +2.4 776,640 - tolA b0739 0.85 +1.4 776,657 - tolA b0739 0.86 +2.4 776,713 - +2.4 776,725 + +2.4 776,779 - +2.4 776,943 + -0.1 776,968 - -0.6 776,994 + +2.4 776,994 + +2.4 777,012 + +2.4 777,014 + +2.4 777,135 + tolB b0740 0.13 +2.4 777,146 + tolB b0740 0.14 +1.4 777,260 + tolB b0740 0.23 +1.4 777,278 - tolB b0740 0.24 +1.4 777,299 - tolB b0740 0.26 +1.4 777,303 - tolB b0740 0.26 +2.4 777,393 - tolB b0740 0.33 +2.4 777,398 - tolB b0740 0.34 +2.4 777,444 - tolB b0740 0.37 +2.4 777,513 + tolB b0740 0.43 +2.4 777,522 - tolB b0740 0.43 +2.4 777,602 - tolB b0740 0.49 +2.4 777,602 - tolB b0740 0.49 +1.4 777,692 + tolB b0740 0.56 +2.4 777,700 - tolB b0740 0.57 +0.8 777,720 - tolB b0740 0.59 +2.4 777,770 - tolB b0740 0.62 +0.4 777,792 - tolB b0740 0.64 +2.4
Or see this region's nucleotide sequence