Experiment: M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagK and yagL are separated by 271 nucleotides yagL and yagM are separated by 26 nucleotides yagM and yagN are separated by 339 nucleotides yagN and intF are separated by 116 nucleotides
b0277: yagK - CP4-6 prophage; conserved protein (NCBI), at 291,546 to 292,172
yagK
b0278: yagL - CP4-6 prophage; DNA-binding protein (NCBI), at 292,444 to 293,142
yagL
b0279: yagM - CP4-6 prophage; predicted protein (NCBI), at 293,169 to 294,023
yagM
b0280: yagN - CP4-6 prophage; predicted protein (NCBI), at 294,363 to 294,803
yagN
b0281: intF - CP4-6 prophage; predicted phage integrase (NCBI), at 294,920 to 296,320
intF
Position (kb) 293
294
295 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 292.174 kb on + strand at 292.182 kb on - strand at 292.191 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on - strand at 292.201 kb on + strand at 292.201 kb on + strand at 292.201 kb on - strand at 292.201 kb on - strand at 292.203 kb on + strand at 292.206 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.209 kb on - strand at 292.209 kb on - strand at 292.209 kb on - strand at 292.210 kb on + strand at 292.210 kb on + strand at 292.211 kb on + strand at 292.211 kb on + strand at 292.211 kb on + strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.213 kb on + strand at 292.213 kb on + strand at 292.213 kb on + strand at 292.218 kb on + strand at 292.218 kb on - strand at 292.218 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on - strand at 292.221 kb on - strand at 292.221 kb on - strand at 292.221 kb on - strand at 292.223 kb on + strand at 292.224 kb on + strand at 292.225 kb on + strand at 292.225 kb on + strand at 292.226 kb on + strand at 292.226 kb on - strand at 292.226 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.231 kb on - strand at 292.231 kb on - strand at 292.231 kb on - strand at 292.231 kb on - strand at 292.232 kb on - strand at 292.232 kb on - strand at 292.233 kb on - strand at 292.235 kb on + strand at 292.235 kb on + strand at 292.238 kb on - strand at 292.244 kb on - strand at 292.260 kb on + strand at 292.260 kb on + strand at 292.260 kb on + strand at 292.288 kb on + strand at 292.291 kb on + strand at 292.291 kb on + strand at 292.296 kb on + strand at 292.301 kb on + strand at 292.301 kb on + strand at 292.312 kb on + strand at 292.317 kb on + strand at 292.317 kb on + strand at 292.334 kb on + strand at 292.388 kb on + strand at 292.395 kb on - strand at 292.395 kb on - strand at 292.400 kb on - strand at 292.400 kb on - strand at 292.410 kb on - strand at 292.410 kb on - strand at 292.471 kb on - strand at 292.481 kb on - strand at 292.490 kb on - strand at 292.490 kb on - strand at 292.519 kb on - strand, within yagL at 292.553 kb on - strand, within yagL at 292.553 kb on - strand, within yagL at 292.620 kb on - strand, within yagL at 292.620 kb on - strand, within yagL at 292.631 kb on - strand, within yagL at 292.631 kb on - strand, within yagL at 292.651 kb on - strand, within yagL at 292.651 kb on - strand, within yagL at 292.686 kb on - strand, within yagL at 292.688 kb on - strand, within yagL at 292.693 kb on - strand, within yagL at 292.697 kb on + strand, within yagL at 292.700 kb on - strand, within yagL at 292.707 kb on - strand, within yagL at 292.717 kb on - strand, within yagL at 292.717 kb on - strand, within yagL at 292.717 kb on - strand, within yagL at 292.718 kb on - strand, within yagL at 292.718 kb on - strand, within yagL at 292.718 kb on - strand, within yagL at 292.782 kb on + strand, within yagL at 292.786 kb on - strand, within yagL at 292.786 kb on - strand, within yagL at 292.786 kb on - strand, within yagL at 292.786 kb on - strand, within yagL at 292.786 kb on - strand, within yagL at 292.790 kb on - strand, within yagL at 292.792 kb on + strand, within yagL at 292.792 kb on + strand, within yagL at 292.792 kb on + strand, within yagL at 292.801 kb on - strand, within yagL at 292.801 kb on - strand, within yagL at 292.805 kb on - strand, within yagL at 292.805 kb on - strand, within yagL at 292.806 kb on - strand, within yagL at 292.806 kb on - strand, within yagL at 292.807 kb on - strand, within yagL at 292.811 kb on + strand, within yagL at 292.830 kb on + strand, within yagL at 292.852 kb on + strand, within yagL at 292.860 kb on - strand, within yagL at 292.871 kb on - strand, within yagL at 292.878 kb on + strand, within yagL at 292.886 kb on - strand, within yagL at 292.904 kb on - strand, within yagL at 292.906 kb on - strand, within yagL at 292.907 kb on + strand, within yagL at 292.917 kb on - strand, within yagL at 292.940 kb on - strand, within yagL at 292.947 kb on - strand, within yagL at 292.947 kb on - strand, within yagL at 292.947 kb on - strand, within yagL at 292.954 kb on - strand, within yagL at 292.954 kb on - strand, within yagL at 292.954 kb on - strand, within yagL at 292.955 kb on - strand, within yagL at 292.969 kb on - strand, within yagL at 292.977 kb on - strand, within yagL at 292.990 kb on - strand, within yagL at 292.990 kb on - strand, within yagL at 292.990 kb on - strand, within yagL at 293.025 kb on - strand, within yagL at 293.029 kb on - strand, within yagL at 293.174 kb on - strand at 293.174 kb on - strand at 293.176 kb on - strand at 293.191 kb on - strand at 293.218 kb on - strand at 293.245 kb on - strand at 293.254 kb on + strand at 293.255 kb on - strand, within yagM at 293.255 kb on - strand, within yagM at 293.673 kb on + strand, within yagM at 293.678 kb on + strand, within yagM at 293.678 kb on + strand, within yagM at 293.820 kb on - strand, within yagM at 293.928 kb on + strand, within yagM at 293.928 kb on + strand, within yagM at 293.933 kb on + strand, within yagM at 293.936 kb on + strand, within yagM at 293.937 kb on - strand, within yagM at 293.938 kb on + strand at 293.938 kb on + strand at 293.938 kb on + strand at 293.960 kb on - strand at 294.107 kb on - strand at 294.107 kb on - strand at 294.195 kb on - strand at 294.200 kb on - strand at 294.200 kb on - strand at 294.206 kb on - strand at 294.206 kb on - strand at 294.225 kb on - strand at 294.232 kb on + strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.275 kb on - strand at 294.330 kb on - strand at 294.338 kb on - strand at 294.387 kb on - strand at 294.387 kb on - strand at 294.389 kb on - strand at 294.389 kb on - strand at 294.423 kb on + strand, within yagN at 294.453 kb on - strand, within yagN at 294.453 kb on - strand, within yagN at 294.466 kb on + strand, within yagN at 294.470 kb on - strand, within yagN at 294.480 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.490 kb on + strand, within yagN at 294.491 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.531 kb on + strand, within yagN at 294.539 kb on - strand, within yagN at 294.663 kb on - strand, within yagN at 294.667 kb on + strand, within yagN at 294.686 kb on - strand, within yagN at 294.698 kb on + strand, within yagN at 294.761 kb on - strand at 294.761 kb on - strand at 294.777 kb on + strand at 294.809 kb on + strand at 294.822 kb on - strand at 294.847 kb on + strand at 294.855 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM remove 292,174 + +1.3 292,182 - +0.3 292,191 + +0.5 292,199 + +4.3 292,199 + +2.3 292,199 + +0.7 292,199 + +1.3 292,199 + +0.7 292,199 + +2.3 292,199 - +0.3 292,201 + -0.3 292,201 + -0.7 292,201 - -1.3 292,201 - -0.6 292,203 + +2.1 292,206 - -0.3 292,207 - -0.1 292,207 - -0.3 292,207 - -0.1 292,207 - +0.8 292,207 - -0.3 292,207 - -0.2 292,207 - +0.9 292,207 - -0.1 292,207 - +1.3 292,207 - -0.7 292,207 - -0.4 292,207 - -0.0 292,207 - -0.6 292,207 - +0.0 292,207 - -0.2 292,207 - -0.3 292,207 - -0.6 292,207 - -0.7 292,207 - -0.3 292,207 - -1.2 292,207 - -0.4 292,207 - +0.6 292,207 - +0.6 292,207 - -0.3 292,207 - +0.5 292,207 - -0.3 292,207 - -0.5 292,209 - -1.8 292,209 - +0.3 292,209 - -0.5 292,210 + +2.3 292,210 + +1.3 292,211 + +2.3 292,211 + +2.3 292,211 + +1.3 292,211 - -1.3 292,211 - +1.6 292,211 - -0.0 292,211 - -0.8 292,211 - -0.6 292,211 - +0.7 292,211 - -0.1 292,211 - -0.2 292,211 - -0.4 292,211 - -0.7 292,211 - -0.8 292,211 - +0.1 292,213 + +2.3 292,213 + +0.7 292,213 + +2.3 292,218 + -1.1 292,218 - +0.6 292,218 - -0.3 292,219 - -1.7 292,219 - +0.1 292,219 - -0.1 292,219 - -0.5 292,219 - -1.9 292,221 + +0.4 292,221 + -0.7 292,221 + +0.6 292,221 + +0.8 292,221 + +1.2 292,221 + +1.6 292,221 + -0.7 292,221 - +1.1 292,221 - -1.3 292,221 - -0.3 292,221 - +0.7 292,223 + -1.4 292,224 + +1.3 292,225 + -0.1 292,225 + +2.3 292,226 + +1.8 292,226 - +0.1 292,226 - +0.8 292,229 - -1.2 292,229 - -0.3 292,229 - +0.3 292,229 - +1.3 292,229 - +1.2 292,231 - -0.1 292,231 - +0.6 292,231 - +2.1 292,231 - +0.3 292,232 - +0.3 292,232 - +0.9 292,233 - -1.1 292,235 + +2.3 292,235 + -0.1 292,238 - +2.3 292,244 - +2.3 292,260 + +2.3 292,260 + +1.3 292,260 + +2.3 292,288 + -1.1 292,291 + +1.3 292,291 + +1.9 292,296 + +0.7 292,301 + +2.7 292,301 + +0.7 292,312 + +2.3 292,317 + +2.3 292,317 + +2.3 292,334 + +2.3 292,388 + +1.3 292,395 - +2.3 292,395 - +2.3 292,400 - +2.3 292,400 - +2.3 292,410 - +0.7 292,410 - +2.3 292,471 - -0.3 292,481 - +0.7 292,490 - -0.3 292,490 - +2.3 292,519 - yagL b0278 0.11 +1.3 292,553 - yagL b0278 0.16 -1.2 292,553 - yagL b0278 0.16 -0.1 292,620 - yagL b0278 0.25 +0.7 292,620 - yagL b0278 0.25 +1.8 292,631 - yagL b0278 0.27 -2.0 292,631 - yagL b0278 0.27 +1.1 292,651 - yagL b0278 0.30 +0.1 292,651 - yagL b0278 0.30 -2.2 292,686 - yagL b0278 0.35 -0.5 292,688 - yagL b0278 0.35 +3.6 292,693 - yagL b0278 0.36 +0.5 292,697 + yagL b0278 0.36 +2.3 292,700 - yagL b0278 0.37 +0.6 292,707 - yagL b0278 0.38 -0.0 292,717 - yagL b0278 0.39 -1.5 292,717 - yagL b0278 0.39 -0.3 292,717 - yagL b0278 0.39 +0.4 292,718 - yagL b0278 0.39 -0.6 292,718 - yagL b0278 0.39 +0.1 292,718 - yagL b0278 0.39 -0.0 292,782 + yagL b0278 0.48 +0.9 292,786 - yagL b0278 0.49 -1.0 292,786 - yagL b0278 0.49 +0.6 292,786 - yagL b0278 0.49 -0.0 292,786 - yagL b0278 0.49 -0.0 292,786 - yagL b0278 0.49 -0.2 292,790 - yagL b0278 0.49 +1.7 292,792 + yagL b0278 0.50 -0.0 292,792 + yagL b0278 0.50 +0.4 292,792 + yagL b0278 0.50 +0.7 292,801 - yagL b0278 0.51 +1.9 292,801 - yagL b0278 0.51 +0.7 292,805 - yagL b0278 0.52 +2.8 292,805 - yagL b0278 0.52 +1.3 292,806 - yagL b0278 0.52 +0.7 292,806 - yagL b0278 0.52 +1.3 292,807 - yagL b0278 0.52 -0.1 292,811 + yagL b0278 0.53 -0.7 292,830 + yagL b0278 0.55 +0.8 292,852 + yagL b0278 0.58 +2.3 292,860 - yagL b0278 0.60 +0.7 292,871 - yagL b0278 0.61 +2.3 292,878 + yagL b0278 0.62 +2.3 292,886 - yagL b0278 0.63 +2.3 292,904 - yagL b0278 0.66 +0.2 292,906 - yagL b0278 0.66 +0.3 292,907 + yagL b0278 0.66 -1.2 292,917 - yagL b0278 0.68 +0.7 292,940 - yagL b0278 0.71 -0.5 292,947 - yagL b0278 0.72 +0.5 292,947 - yagL b0278 0.72 -0.0 292,947 - yagL b0278 0.72 +0.0 292,954 - yagL b0278 0.73 +1.5 292,954 - yagL b0278 0.73 +2.3 292,954 - yagL b0278 0.73 +0.3 292,955 - yagL b0278 0.73 +0.7 292,969 - yagL b0278 0.75 -1.3 292,977 - yagL b0278 0.76 +2.3 292,990 - yagL b0278 0.78 -0.1 292,990 - yagL b0278 0.78 +2.3 292,990 - yagL b0278 0.78 +0.1 293,025 - yagL b0278 0.83 +0.7 293,029 - yagL b0278 0.84 +0.7 293,174 - +1.0 293,174 - -0.3 293,176 - +1.3 293,191 - +1.3 293,218 - +2.3 293,245 - +2.3 293,254 + +2.3 293,255 - yagM b0279 0.10 +2.3 293,255 - yagM b0279 0.10 +1.3 293,673 + yagM b0279 0.59 +2.3 293,678 + yagM b0279 0.60 +2.3 293,678 + yagM b0279 0.60 +2.3 293,820 - yagM b0279 0.76 +2.3 293,928 + yagM b0279 0.89 +0.3 293,928 + yagM b0279 0.89 +1.3 293,933 + yagM b0279 0.89 +2.3 293,936 + yagM b0279 0.90 +1.3 293,937 - yagM b0279 0.90 +2.3 293,938 + +1.3 293,938 + +0.7 293,938 + +2.3 293,960 - +0.7 294,107 - +0.1 294,107 - -0.3 294,195 - +0.1 294,200 - -1.0 294,200 - +0.3 294,206 - -0.1 294,206 - +1.3 294,225 - -0.0 294,232 + +2.3 294,240 - +0.0 294,240 - -0.7 294,240 - -0.4 294,240 - -0.4 294,275 - -0.3 294,330 - +0.3 294,338 - +0.3 294,387 - +0.7 294,387 - +0.5 294,389 - -0.8 294,389 - -0.4 294,423 + yagN b0280 0.14 -1.2 294,453 - yagN b0280 0.20 -0.7 294,453 - yagN b0280 0.20 +0.1 294,466 + yagN b0280 0.23 +0.4 294,470 - yagN b0280 0.24 +0.2 294,480 - yagN b0280 0.27 -0.3 294,488 - yagN b0280 0.28 -0.0 294,488 - yagN b0280 0.28 -0.1 294,488 - yagN b0280 0.28 +0.2 294,490 + yagN b0280 0.29 -0.3 294,491 + yagN b0280 0.29 +0.2 294,523 + yagN b0280 0.36 +1.4 294,523 + yagN b0280 0.36 +0.8 294,523 + yagN b0280 0.36 +0.4 294,531 + yagN b0280 0.38 +0.3 294,539 - yagN b0280 0.40 -2.7 294,663 - yagN b0280 0.68 +0.3 294,667 + yagN b0280 0.69 -0.4 294,686 - yagN b0280 0.73 -0.7 294,698 + yagN b0280 0.76 +0.6 294,761 - +1.6 294,761 - +0.3 294,777 + -1.2 294,809 + -0.4 294,822 - -0.0 294,847 + -0.4 294,855 - +0.5
Or see this region's nucleotide sequence