Strain Fitness in Escherichia coli BW25113 around b3940
Experiment: M9 Anaerobic Capecitabine 500 uM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | M9 Anaerobic Capecitabine 500 uM |
---|---|---|---|---|---|
remove | |||||
4,126,900 | + | metB | b3939 | 0.18 | +0.0 |
4,126,900 | + | metB | b3939 | 0.18 | +1.0 |
4,126,902 | - | metB | b3939 | 0.18 | +0.0 |
4,126,908 | - | metB | b3939 | 0.18 | +0.0 |
4,126,910 | + | metB | b3939 | 0.19 | +0.5 |
4,126,913 | - | metB | b3939 | 0.19 | -0.2 |
4,127,015 | + | metB | b3939 | 0.28 | -0.1 |
4,127,023 | + | metB | b3939 | 0.28 | +0.9 |
4,127,042 | + | metB | b3939 | 0.30 | +1.0 |
4,127,042 | + | metB | b3939 | 0.30 | +0.1 |
4,127,112 | - | metB | b3939 | 0.36 | +0.2 |
4,127,120 | + | metB | b3939 | 0.37 | +0.3 |
4,127,152 | - | metB | b3939 | 0.39 | +1.2 |
4,127,203 | - | metB | b3939 | 0.44 | -0.1 |
4,127,232 | + | metB | b3939 | 0.46 | +0.5 |
4,127,273 | + | metB | b3939 | 0.50 | -0.6 |
4,127,273 | + | metB | b3939 | 0.50 | +0.5 |
4,127,373 | - | metB | b3939 | 0.58 | -0.8 |
4,127,400 | + | metB | b3939 | 0.61 | -0.1 |
4,127,400 | + | metB | b3939 | 0.61 | +0.8 |
4,127,410 | + | metB | b3939 | 0.62 | +0.5 |
4,127,453 | - | metB | b3939 | 0.65 | +0.2 |
4,127,592 | - | metB | b3939 | 0.77 | +0.0 |
4,127,604 | - | metB | b3939 | 0.78 | -0.0 |
4,127,663 | + | metB | b3939 | 0.83 | -0.1 |
4,127,663 | + | metB | b3939 | 0.83 | -0.2 |
4,127,709 | + | metB | b3939 | 0.87 | -0.0 |
4,127,719 | + | metB | b3939 | 0.88 | +0.5 |
4,127,720 | + | metB | b3939 | 0.88 | -0.5 |
4,127,720 | + | metB | b3939 | 0.88 | -0.1 |
4,127,725 | + | metB | b3939 | 0.89 | -0.3 |
4,127,737 | - | metB | b3939 | 0.90 | +0.0 |
4,127,737 | - | metB | b3939 | 0.90 | -0.5 |
4,127,788 | + | -0.6 | |||
4,127,796 | - | +0.3 | |||
4,127,796 | - | -0.5 | |||
4,127,796 | - | -0.8 | |||
4,127,926 | - | +1.5 | |||
4,127,978 | - | +1.5 | |||
4,128,062 | - | +1.5 | |||
4,128,238 | - | metL | b3940 | 0.16 | +1.5 |
4,128,370 | + | metL | b3940 | 0.21 | +0.5 |
4,128,487 | + | metL | b3940 | 0.26 | +1.5 |
4,129,055 | + | metL | b3940 | 0.49 | +1.5 |
4,129,114 | - | metL | b3940 | 0.52 | +1.5 |
4,129,155 | + | metL | b3940 | 0.53 | +1.5 |
4,129,197 | - | metL | b3940 | 0.55 | +1.5 |
4,129,201 | + | metL | b3940 | 0.55 | +1.3 |
4,129,236 | + | metL | b3940 | 0.57 | -0.2 |
4,129,236 | + | metL | b3940 | 0.57 | +0.2 |
4,129,236 | + | metL | b3940 | 0.57 | -0.2 |
4,129,243 | - | metL | b3940 | 0.57 | -0.1 |
4,129,244 | - | metL | b3940 | 0.57 | -0.7 |
4,129,283 | - | metL | b3940 | 0.59 | +1.5 |
4,129,290 | + | metL | b3940 | 0.59 | -0.5 |
4,129,317 | + | metL | b3940 | 0.60 | -0.1 |
4,129,332 | - | metL | b3940 | 0.61 | -0.1 |
4,129,367 | + | metL | b3940 | 0.62 | -0.6 |
4,129,444 | + | metL | b3940 | 0.65 | -2.2 |
4,129,444 | + | metL | b3940 | 0.65 | -0.3 |
4,129,444 | - | metL | b3940 | 0.65 | +1.5 |
4,129,444 | - | metL | b3940 | 0.65 | -2.1 |
4,129,452 | - | metL | b3940 | 0.66 | -0.1 |
4,129,472 | - | metL | b3940 | 0.66 | +0.5 |
4,129,472 | - | metL | b3940 | 0.66 | +1.5 |
4,129,498 | - | metL | b3940 | 0.67 | -0.5 |
4,129,596 | + | metL | b3940 | 0.71 | -0.9 |
4,129,607 | + | metL | b3940 | 0.72 | +0.3 |
4,129,607 | + | metL | b3940 | 0.72 | -1.1 |
4,129,641 | + | metL | b3940 | 0.73 | -1.9 |
4,129,641 | + | metL | b3940 | 0.73 | -0.5 |
4,129,649 | - | metL | b3940 | 0.74 | -1.1 |
4,129,649 | - | metL | b3940 | 0.74 | +1.5 |
4,129,656 | - | metL | b3940 | 0.74 | -1.9 |
4,129,684 | + | metL | b3940 | 0.75 | -1.2 |
4,129,684 | + | metL | b3940 | 0.75 | +0.0 |
4,129,684 | + | metL | b3940 | 0.75 | +0.8 |
4,129,690 | - | metL | b3940 | 0.75 | -1.3 |
4,129,690 | - | metL | b3940 | 0.75 | +0.1 |
4,129,722 | + | metL | b3940 | 0.77 | -0.5 |
4,129,759 | + | metL | b3940 | 0.78 | +2.1 |
4,129,759 | + | metL | b3940 | 0.78 | -0.1 |
4,129,759 | + | metL | b3940 | 0.78 | +0.5 |
4,129,759 | + | metL | b3940 | 0.78 | +1.5 |
4,129,783 | + | metL | b3940 | 0.79 | -1.7 |
4,129,805 | + | metL | b3940 | 0.80 | -0.4 |
4,129,811 | + | metL | b3940 | 0.80 | -0.2 |
4,129,830 | + | metL | b3940 | 0.81 | -0.1 |
4,129,836 | - | metL | b3940 | 0.81 | -0.1 |
4,129,866 | + | metL | b3940 | 0.83 | -0.1 |
4,129,866 | + | metL | b3940 | 0.83 | -0.3 |
4,129,879 | + | metL | b3940 | 0.83 | -0.2 |
4,129,879 | + | metL | b3940 | 0.83 | -1.6 |
4,129,925 | - | metL | b3940 | 0.85 | +0.5 |
4,129,928 | + | metL | b3940 | 0.85 | -0.2 |
4,129,928 | + | metL | b3940 | 0.85 | -0.4 |
4,129,938 | + | metL | b3940 | 0.85 | +1.1 |
4,129,938 | + | metL | b3940 | 0.85 | -0.6 |
4,129,953 | + | metL | b3940 | 0.86 | -0.9 |
4,130,020 | - | metL | b3940 | 0.89 | -1.3 |
4,130,079 | + | -0.4 | |||
4,130,097 | - | +0.4 | |||
4,130,097 | - | +0.5 | |||
4,130,116 | - | -0.5 | |||
4,130,194 | + | -0.6 | |||
4,130,196 | - | +0.5 | |||
4,130,202 | - | -1.2 | |||
4,130,202 | - | -1.1 | |||
4,130,211 | + | -0.1 | |||
4,130,219 | + | -0.1 | |||
4,130,229 | + | -1.2 | |||
4,130,284 | - | +0.0 | |||
4,130,309 | + | -0.1 | |||
4,130,314 | + | +0.1 | |||
4,130,325 | - | -0.0 | |||
4,130,330 | - | -0.2 | |||
4,130,378 | + | -0.3 | |||
4,130,378 | + | +0.4 | |||
4,130,386 | - | -0.1 | |||
4,130,416 | + | -0.0 | |||
4,130,479 | - | +0.2 | |||
4,130,523 | - | -0.8 | |||
4,130,609 | - | +0.3 | |||
4,130,609 | - | -0.5 | |||
4,130,614 | - | -0.4 | |||
4,130,620 | - | -1.0 | |||
4,130,631 | + | -0.7 | |||
4,130,631 | + | -0.4 | |||
4,130,678 | - | -0.8 | |||
4,130,690 | - | -1.7 | |||
4,130,787 | + | metF | b3941 | 0.17 | -1.3 |
4,130,787 | + | metF | b3941 | 0.17 | -0.3 |
4,130,795 | - | metF | b3941 | 0.18 | -1.3 |
4,130,849 | - | metF | b3941 | 0.24 | -0.8 |
4,130,853 | + | metF | b3941 | 0.24 | -0.3 |
4,130,853 | + | metF | b3941 | 0.24 | -1.0 |
4,130,856 | + | metF | b3941 | 0.24 | -1.2 |
4,130,861 | - | metF | b3941 | 0.25 | -1.5 |
4,130,882 | + | metF | b3941 | 0.27 | -1.5 |
4,130,883 | + | metF | b3941 | 0.27 | -0.7 |
4,130,885 | - | metF | b3941 | 0.28 | -0.7 |
4,130,885 | - | metF | b3941 | 0.28 | -1.8 |
4,130,940 | + | metF | b3941 | 0.34 | -1.2 |
4,131,034 | - | metF | b3941 | 0.44 | -1.1 |
4,131,079 | + | metF | b3941 | 0.49 | -1.3 |
4,131,236 | - | metF | b3941 | 0.67 | -0.5 |
4,131,286 | - | metF | b3941 | 0.73 | -1.5 |
Or see this region's nucleotide sequence