Strain Fitness in Escherichia coli BW25113 around b2319

Experiment: M9 Anaerobic Capecitabine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdedA and truA are separated by 82 nucleotidestruA and usg overlap by 1 nucleotidesusg and pdxB are separated by 65 nucleotides b2317: dedA - conserved inner membrane protein (NCBI), at 2,432,104 to 2,432,763 dedA b2318: truA - tRNA pseudouridine synthase A (NCBI), at 2,432,846 to 2,433,658 truA b2319: usg - hypothetical protein (NCBI), at 2,433,658 to 2,434,671 usg b2320: pdxB - erythronate-4-phosphate dehydrogenase (NCBI), at 2,434,737 to 2,435,873 pdxB Position (kb) 2433 2434 2435Strain fitness (log2 ratio) -2 -1 0 1 2at 2432.800 kb on + strandat 2432.808 kb on - strandat 2432.808 kb on - strandat 2433.004 kb on + strand, within truAat 2433.141 kb on + strand, within truAat 2433.141 kb on + strand, within truAat 2433.157 kb on + strand, within truAat 2433.160 kb on - strand, within truAat 2433.160 kb on - strand, within truAat 2433.170 kb on - strand, within truAat 2433.363 kb on - strandat 2433.465 kb on - strand, within truAat 2433.465 kb on - strand, within truAat 2433.465 kb on - strand, within truAat 2433.465 kb on - strand, within truAat 2433.600 kb on + strandat 2433.600 kb on + strandat 2433.656 kb on + strandat 2433.664 kb on - strandat 2433.670 kb on + strandat 2433.670 kb on + strandat 2433.678 kb on - strandat 2433.753 kb on - strandat 2433.846 kb on + strand, within usgat 2433.853 kb on - strand, within usgat 2433.853 kb on - strand, within usgat 2433.910 kb on + strand, within usgat 2433.943 kb on + strand, within usgat 2433.952 kb on - strand, within usgat 2433.952 kb on - strand, within usgat 2434.057 kb on + strand, within usgat 2434.167 kb on - strand, within usgat 2434.287 kb on - strand, within usgat 2434.287 kb on - strand, within usgat 2434.339 kb on + strand, within usgat 2434.360 kb on + strand, within usgat 2434.626 kb on - strandat 2434.647 kb on + strandat 2434.647 kb on + strandat 2434.690 kb on - strandat 2434.711 kb on + strandat 2434.761 kb on + strandat 2434.787 kb on + strandat 2434.881 kb on - strand, within pdxBat 2434.881 kb on - strand, within pdxBat 2434.932 kb on + strand, within pdxBat 2434.932 kb on + strand, within pdxBat 2434.999 kb on - strand, within pdxBat 2435.038 kb on - strand, within pdxBat 2435.105 kb on - strandat 2435.110 kb on + strand, within pdxBat 2435.110 kb on + strand, within pdxBat 2435.230 kb on + strand, within pdxBat 2435.230 kb on + strand, within pdxBat 2435.246 kb on + strand, within pdxBat 2435.285 kb on + strand, within pdxBat 2435.324 kb on + strand, within pdxBat 2435.324 kb on + strand, within pdxBat 2435.343 kb on - strand, within pdxBat 2435.511 kb on + strand, within pdxB

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic Capecitabine 500 uM
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2,432,800 + -0.6
2,432,808 - -0.3
2,432,808 - +1.0
2,433,004 + truA b2318 0.19 -2.3
2,433,141 + truA b2318 0.36 -2.0
2,433,141 + truA b2318 0.36 -1.7
2,433,157 + truA b2318 0.38 -1.1
2,433,160 - truA b2318 0.39 -0.4
2,433,160 - truA b2318 0.39 +0.7
2,433,170 - truA b2318 0.40 -0.1
2,433,363 - +0.4
2,433,465 - truA b2318 0.76 -2.5
2,433,465 - truA b2318 0.76 -1.9
2,433,465 - truA b2318 0.76 -0.9
2,433,465 - truA b2318 0.76 -2.0
2,433,600 + -0.1
2,433,600 + +0.0
2,433,656 + -0.3
2,433,664 - -0.2
2,433,670 + +2.4
2,433,670 + -1.1
2,433,678 - -1.0
2,433,753 - +0.1
2,433,846 + usg b2319 0.19 -2.3
2,433,853 - usg b2319 0.19 -0.0
2,433,853 - usg b2319 0.19 -1.3
2,433,910 + usg b2319 0.25 +0.2
2,433,943 + usg b2319 0.28 +0.4
2,433,952 - usg b2319 0.29 -0.4
2,433,952 - usg b2319 0.29 -0.0
2,434,057 + usg b2319 0.39 -0.1
2,434,167 - usg b2319 0.50 +0.6
2,434,287 - usg b2319 0.62 -0.1
2,434,287 - usg b2319 0.62 -1.8
2,434,339 + usg b2319 0.67 -0.0
2,434,360 + usg b2319 0.69 +1.2
2,434,626 - -0.3
2,434,647 + -0.4
2,434,647 + -2.1
2,434,690 - -0.3
2,434,711 + +0.2
2,434,761 + -0.7
2,434,787 + +0.1
2,434,881 - pdxB b2320 0.13 -1.7
2,434,881 - pdxB b2320 0.13 -1.4
2,434,932 + pdxB b2320 0.17 -0.9
2,434,932 + pdxB b2320 0.17 -1.1
2,434,999 - pdxB b2320 0.23 -2.0
2,435,038 - pdxB b2320 0.26 -1.7
2,435,105 - -2.3
2,435,110 + pdxB b2320 0.33 -1.9
2,435,110 + pdxB b2320 0.33 -1.7
2,435,230 + pdxB b2320 0.43 -0.7
2,435,230 + pdxB b2320 0.43 -1.4
2,435,246 + pdxB b2320 0.45 -1.9
2,435,285 + pdxB b2320 0.48 -1.4
2,435,324 + pdxB b2320 0.52 -1.4
2,435,324 + pdxB b2320 0.52 -1.5
2,435,343 - pdxB b2320 0.53 -1.6
2,435,511 + pdxB b2320 0.68 -1.0

Or see this region's nucleotide sequence