Strain Fitness in Escherichia coli BW25113 around b2508

Experiment: M9 Anaerobic Capecitabine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntguaA and guaB are separated by 68 nucleotidesguaB and xseA are separated by 161 nucleotides b2507: guaA - bifunctional GMP synthase/glutamine amidotransferase protein (NCBI), at 2,628,980 to 2,630,557 guaA b2508: guaB - inositol-5-monophosphate dehydrogenase (NCBI), at 2,630,626 to 2,632,092 guaB b2509: xseA - exodeoxyribonuclease VII large subunit (NCBI), at 2,632,254 to 2,633,624 xseA Position (kb) 2630 2631 2632 2633Strain fitness (log2 ratio) -1 0 1at 2630.612 kb on - strandat 2632.102 kb on - strandat 2632.142 kb on - strandat 2632.263 kb on + strandat 2632.263 kb on + strandat 2632.271 kb on - strandat 2632.317 kb on - strandat 2632.419 kb on + strand, within xseAat 2632.419 kb on + strand, within xseAat 2632.555 kb on + strand, within xseAat 2632.578 kb on - strand, within xseAat 2632.658 kb on + strand, within xseAat 2632.658 kb on + strand, within xseAat 2632.670 kb on + strand, within xseAat 2632.670 kb on + strand, within xseAat 2632.688 kb on - strand, within xseAat 2632.688 kb on - strandat 2632.729 kb on + strand, within xseAat 2632.729 kb on + strand, within xseAat 2632.771 kb on - strand, within xseAat 2632.911 kb on + strand, within xseAat 2632.969 kb on + strand, within xseAat 2633.042 kb on - strand, within xseA

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic Capecitabine 500 uM
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2,630,612 - -1.1
2,632,102 - -0.2
2,632,142 - +1.5
2,632,263 + -0.3
2,632,263 + +0.3
2,632,271 - +0.3
2,632,317 - +0.2
2,632,419 + xseA b2509 0.12 +0.3
2,632,419 + xseA b2509 0.12 +0.2
2,632,555 + xseA b2509 0.22 +0.2
2,632,578 - xseA b2509 0.24 -0.2
2,632,658 + xseA b2509 0.29 +0.0
2,632,658 + xseA b2509 0.29 -1.1
2,632,670 + xseA b2509 0.30 -0.1
2,632,670 + xseA b2509 0.30 -0.4
2,632,688 - xseA b2509 0.32 -0.1
2,632,688 - -0.1
2,632,729 + xseA b2509 0.35 -0.2
2,632,729 + xseA b2509 0.35 -0.5
2,632,771 - xseA b2509 0.38 +0.2
2,632,911 + xseA b2509 0.48 -0.5
2,632,969 + xseA b2509 0.52 -0.0
2,633,042 - xseA b2509 0.57 +0.1

Or see this region's nucleotide sequence