Strain Fitness in Escherichia coli BW25113 around b2281

Experiment: M9 Anaerobic Capecitabine 500 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnuoL and nuoK overlap by 4 nucleotidesnuoK and nuoJ overlap by 4 nucleotidesnuoJ and nuoI are separated by 11 nucleotidesnuoI and nuoH are separated by 14 nucleotidesnuoH and nuoG overlap by 4 nucleotides b2278: nuoL - NADH dehydrogenase subunit L (NCBI), at 2,391,227 to 2,393,068 nuoL b2279: nuoK - NADH dehydrogenase subunit K (NCBI), at 2,393,065 to 2,393,367 nuoK b2280: nuoJ - NADH dehydrogenase subunit J (NCBI), at 2,393,364 to 2,393,918 nuoJ b2281: nuoI - NADH dehydrogenase subunit I (NCBI), at 2,393,930 to 2,394,472 nuoI b2282: nuoH - NADH dehydrogenase subunit H (NCBI), at 2,394,487 to 2,395,464 nuoH b2283: nuoG - NADH:ubiquinone oxidoreductase, chain G (RefSeq), at 2,395,461 to 2,398,187 nuoG Position (kb) 2393 2394 2395Strain fitness (log2 ratio) -1 0 1 2at 2393.077 kb on + strandat 2393.077 kb on + strandat 2393.106 kb on + strand, within nuoKat 2393.152 kb on - strand, within nuoKat 2393.213 kb on - strand, within nuoKat 2393.323 kb on + strand, within nuoKat 2393.346 kb on + strandat 2393.410 kb on + strandat 2393.412 kb on + strandat 2393.412 kb on + strandat 2393.419 kb on + strandat 2393.456 kb on - strand, within nuoJat 2393.463 kb on - strand, within nuoJat 2393.530 kb on - strand, within nuoJat 2393.530 kb on - strand, within nuoJat 2393.536 kb on + strand, within nuoJat 2393.559 kb on + strand, within nuoJat 2393.581 kb on - strand, within nuoJat 2393.586 kb on - strand, within nuoJat 2393.606 kb on + strand, within nuoJat 2393.611 kb on - strand, within nuoJat 2393.617 kb on + strand, within nuoJat 2393.637 kb on + strand, within nuoJat 2393.638 kb on + strand, within nuoJat 2393.638 kb on + strand, within nuoJat 2393.645 kb on + strand, within nuoJat 2393.724 kb on + strand, within nuoJat 2393.731 kb on - strand, within nuoJat 2393.839 kb on - strand, within nuoJat 2393.840 kb on + strand, within nuoJat 2393.876 kb on + strandat 2393.898 kb on - strandat 2393.915 kb on + strandat 2393.915 kb on + strandat 2393.967 kb on - strandat 2393.967 kb on - strandat 2394.043 kb on + strand, within nuoIat 2394.124 kb on - strand, within nuoIat 2394.163 kb on + strand, within nuoIat 2394.320 kb on + strand, within nuoIat 2394.328 kb on - strand, within nuoIat 2394.345 kb on - strand, within nuoIat 2394.347 kb on - strand, within nuoIat 2394.411 kb on - strand, within nuoIat 2394.482 kb on - strandat 2394.495 kb on - strandat 2394.495 kb on - strandat 2394.603 kb on + strand, within nuoHat 2394.603 kb on + strand, within nuoHat 2394.624 kb on + strand, within nuoHat 2394.634 kb on - strand, within nuoHat 2394.712 kb on - strandat 2394.804 kb on + strand, within nuoHat 2394.815 kb on + strand, within nuoHat 2394.834 kb on + strand, within nuoHat 2395.011 kb on + strand, within nuoHat 2395.154 kb on - strand, within nuoHat 2395.213 kb on + strand, within nuoHat 2395.213 kb on + strand, within nuoHat 2395.265 kb on + strand, within nuoHat 2395.375 kb on - strandat 2395.392 kb on - strandat 2395.406 kb on + strandat 2395.415 kb on - strandat 2395.451 kb on - strandat 2395.471 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic Capecitabine 500 uM
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2,393,077 + +1.5
2,393,077 + +0.1
2,393,106 + nuoK b2279 0.14 +2.3
2,393,152 - nuoK b2279 0.29 +0.5
2,393,213 - nuoK b2279 0.49 +0.3
2,393,323 + nuoK b2279 0.85 +0.2
2,393,346 + -0.0
2,393,410 + +0.3
2,393,412 + +0.8
2,393,412 + +0.5
2,393,419 + +1.8
2,393,456 - nuoJ b2280 0.17 -0.5
2,393,463 - nuoJ b2280 0.18 +1.4
2,393,530 - nuoJ b2280 0.30 +0.4
2,393,530 - nuoJ b2280 0.30 +0.8
2,393,536 + nuoJ b2280 0.31 -0.5
2,393,559 + nuoJ b2280 0.35 +0.4
2,393,581 - nuoJ b2280 0.39 -0.1
2,393,586 - nuoJ b2280 0.40 +0.4
2,393,606 + nuoJ b2280 0.44 +0.2
2,393,611 - nuoJ b2280 0.45 +0.5
2,393,617 + nuoJ b2280 0.46 +0.4
2,393,637 + nuoJ b2280 0.49 -0.5
2,393,638 + nuoJ b2280 0.49 +1.2
2,393,638 + nuoJ b2280 0.49 +1.7
2,393,645 + nuoJ b2280 0.51 +0.3
2,393,724 + nuoJ b2280 0.65 +0.1
2,393,731 - nuoJ b2280 0.66 +0.6
2,393,839 - nuoJ b2280 0.86 -0.6
2,393,840 + nuoJ b2280 0.86 +0.3
2,393,876 + -0.5
2,393,898 - -0.6
2,393,915 + +0.3
2,393,915 + +1.0
2,393,967 - +0.2
2,393,967 - +0.0
2,394,043 + nuoI b2281 0.21 -0.1
2,394,124 - nuoI b2281 0.36 +0.4
2,394,163 + nuoI b2281 0.43 +0.3
2,394,320 + nuoI b2281 0.72 -0.1
2,394,328 - nuoI b2281 0.73 +0.7
2,394,345 - nuoI b2281 0.76 +0.7
2,394,347 - nuoI b2281 0.77 -1.4
2,394,411 - nuoI b2281 0.89 +0.7
2,394,482 - -0.0
2,394,495 - -0.4
2,394,495 - +0.2
2,394,603 + nuoH b2282 0.12 +1.1
2,394,603 + nuoH b2282 0.12 +1.5
2,394,624 + nuoH b2282 0.14 +0.5
2,394,634 - nuoH b2282 0.15 +1.0
2,394,712 - -0.1
2,394,804 + nuoH b2282 0.32 +0.8
2,394,815 + nuoH b2282 0.34 -0.5
2,394,834 + nuoH b2282 0.35 +0.9
2,395,011 + nuoH b2282 0.54 +0.8
2,395,154 - nuoH b2282 0.68 +0.2
2,395,213 + nuoH b2282 0.74 +0.7
2,395,213 + nuoH b2282 0.74 -0.9
2,395,265 + nuoH b2282 0.80 +1.0
2,395,375 - +0.7
2,395,392 - -0.8
2,395,406 + +0.9
2,395,415 - -0.9
2,395,451 - +0.5
2,395,471 + +0.9

Or see this region's nucleotide sequence