Experiment: M9 aerobic no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuZ and cysT are separated by 12 nucleotides cysT and glyW are separated by 54 nucleotides glyW and pgsA are separated by 151 nucleotides pgsA and uvrC are separated by 56 nucleotides uvrC and uvrY overlap by 4 nucleotides
b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925
leuZ
b1910: cysT - tRNA-Cys (NCBI), at 1,989,938 to 1,990,011
cysT
b1911: glyW - tRNA-Gly (NCBI), at 1,990,066 to 1,990,141
glyW
b1912: pgsA - phosphatidylglycerophosphate synthetase (NCBI), at 1,990,293 to 1,990,841
pgsA
b1913: uvrC - excinuclease ABC, subunit C; repair of UV damage to DNA (VIMSS), at 1,990,898 to 1,992,730
uvrC
b1914: uvrY - response regulator (NCBI), at 1,992,727 to 1,993,383
uvrY
Position (kb)
1990
1991
1992
1993 Strain fitness (log2 ratio)
-1
0
1 at 1990.916 kb on + strand at 1991.220 kb on + strand, within uvrC at 1991.230 kb on + strand, within uvrC at 1991.528 kb on + strand, within uvrC at 1991.533 kb on + strand, within uvrC at 1991.533 kb on + strand, within uvrC at 1991.662 kb on + strand, within uvrC at 1991.662 kb on + strand, within uvrC at 1991.798 kb on - strand, within uvrC at 1991.798 kb on - strand, within uvrC at 1991.857 kb on + strand, within uvrC at 1991.922 kb on + strand, within uvrC at 1991.948 kb on - strand, within uvrC at 1992.150 kb on + strand, within uvrC at 1992.202 kb on + strand, within uvrC at 1992.227 kb on - strand, within uvrC at 1992.461 kb on - strand, within uvrC at 1992.461 kb on - strand, within uvrC at 1992.523 kb on - strand, within uvrC at 1992.523 kb on - strand, within uvrC at 1992.721 kb on + strand at 1992.862 kb on + strand, within uvrY at 1993.136 kb on - strand, within uvrY at 1993.136 kb on - strand, within uvrY at 1993.220 kb on - strand, within uvrY at 1993.236 kb on + strand, within uvrY at 1993.236 kb on + strand, within uvrY at 1993.244 kb on - strand, within uvrY at 1993.244 kb on - strand, within uvrY at 1993.268 kb on - strand, within uvrY at 1993.309 kb on + strand, within uvrY at 1993.317 kb on + strand, within uvrY at 1993.328 kb on + strand at 1993.345 kb on + strand at 1993.350 kb on + strand at 1993.350 kb on + strand at 1993.358 kb on - strand at 1993.415 kb on - strand at 1993.419 kb on + strand at 1993.419 kb on - strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.534 kb on + strand at 1993.537 kb on + strand at 1993.540 kb on - strand at 1993.542 kb on - strand at 1993.548 kb on - strand at 1993.548 kb on - strand at 1993.549 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.560 kb on + strand at 1993.560 kb on + strand at 1993.565 kb on + strand at 1993.571 kb on + strand at 1993.571 kb on + strand at 1993.571 kb on + strand at 1993.717 kb on + strand at 1993.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 aerobic no stress control remove 1,990,916 + -1.1 1,991,220 + uvrC b1913 0.18 +0.8 1,991,230 + uvrC b1913 0.18 +0.4 1,991,528 + uvrC b1913 0.34 +0.1 1,991,533 + uvrC b1913 0.35 -1.1 1,991,533 + uvrC b1913 0.35 -0.5 1,991,662 + uvrC b1913 0.42 +0.4 1,991,662 + uvrC b1913 0.42 -0.1 1,991,798 - uvrC b1913 0.49 +0.2 1,991,798 - uvrC b1913 0.49 +0.0 1,991,857 + uvrC b1913 0.52 +0.7 1,991,922 + uvrC b1913 0.56 -0.6 1,991,948 - uvrC b1913 0.57 +0.0 1,992,150 + uvrC b1913 0.68 -0.0 1,992,202 + uvrC b1913 0.71 +0.2 1,992,227 - uvrC b1913 0.73 -0.3 1,992,461 - uvrC b1913 0.85 -0.6 1,992,461 - uvrC b1913 0.85 +0.1 1,992,523 - uvrC b1913 0.89 +1.7 1,992,523 - uvrC b1913 0.89 -0.9 1,992,721 + -0.4 1,992,862 + uvrY b1914 0.21 -0.3 1,993,136 - uvrY b1914 0.62 +0.0 1,993,136 - uvrY b1914 0.62 -0.7 1,993,220 - uvrY b1914 0.75 +0.8 1,993,236 + uvrY b1914 0.77 +0.2 1,993,236 + uvrY b1914 0.77 +0.3 1,993,244 - uvrY b1914 0.79 -0.1 1,993,244 - uvrY b1914 0.79 +0.1 1,993,268 - uvrY b1914 0.82 -0.0 1,993,309 + uvrY b1914 0.89 -0.3 1,993,317 + uvrY b1914 0.90 -0.0 1,993,328 + -0.7 1,993,345 + +0.1 1,993,350 + -0.3 1,993,350 + -0.2 1,993,358 - -1.2 1,993,415 - -0.8 1,993,419 + +0.0 1,993,419 - -0.2 1,993,522 + -0.2 1,993,522 + +0.3 1,993,522 + +0.1 1,993,522 + -0.6 1,993,534 + +0.9 1,993,537 + +0.6 1,993,540 - +0.8 1,993,542 - +0.8 1,993,548 - +0.8 1,993,548 - +0.8 1,993,549 + -0.7 1,993,559 + +1.0 1,993,559 + +0.2 1,993,559 + -0.4 1,993,559 + +0.2 1,993,559 + -0.5 1,993,559 + +0.1 1,993,560 + -0.4 1,993,560 + -0.0 1,993,565 + -0.2 1,993,571 + -0.7 1,993,571 + +0.7 1,993,571 + +0.3 1,993,717 + -1.2 1,993,717 + -1.2
Or see this region's nucleotide sequence