Experiment: M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zntA and yhhP are separated by 101 nucleotides yhhP and yhhQ are separated by 220 nucleotides yhhQ and b3472 are separated by 72 nucleotides
b3469: zntA - zinc, cobalt and lead efflux system (NCBI), at 3,604,474 to 3,606,672
zntA
b3470: yhhP - cell developmental protein SirA (NCBI), at 3,606,774 to 3,607,019
yhhP
b3471: yhhQ - conserved inner membrane protein (NCBI), at 3,607,240 to 3,607,905
yhhQ
b3472: b3472 - orf, hypothetical protein (VIMSS), at 3,607,978 to 3,608,535
b3472
Position (kb)
3606
3607
3608 Strain fitness (log2 ratio)
-2
-1
0
1 at 3605.846 kb on - strand, within zntA at 3605.849 kb on + strand, within zntA at 3605.870 kb on - strand, within zntA at 3605.905 kb on + strand, within zntA at 3605.905 kb on + strand, within zntA at 3605.909 kb on + strand, within zntA at 3605.932 kb on - strand, within zntA at 3606.008 kb on - strand, within zntA at 3606.008 kb on - strand, within zntA at 3606.008 kb on - strand, within zntA at 3606.021 kb on + strand, within zntA at 3606.069 kb on - strand, within zntA at 3606.074 kb on - strand, within zntA at 3606.078 kb on + strand, within zntA at 3606.100 kb on + strand, within zntA at 3606.100 kb on + strand, within zntA at 3606.144 kb on + strand, within zntA at 3606.198 kb on + strand, within zntA at 3606.203 kb on + strand, within zntA at 3606.222 kb on - strand, within zntA at 3606.298 kb on + strand, within zntA at 3606.326 kb on + strand, within zntA at 3606.331 kb on + strand, within zntA at 3606.350 kb on - strand, within zntA at 3606.350 kb on - strand, within zntA at 3606.367 kb on - strand, within zntA at 3606.367 kb on - strand, within zntA at 3606.367 kb on - strand, within zntA at 3606.383 kb on - strand, within zntA at 3606.383 kb on - strand, within zntA at 3606.414 kb on + strand, within zntA at 3606.414 kb on + strand, within zntA at 3606.448 kb on + strand, within zntA at 3606.448 kb on + strand, within zntA at 3606.454 kb on + strand at 3606.454 kb on + strand at 3606.468 kb on + strand at 3606.478 kb on + strand at 3606.478 kb on + strand at 3606.590 kb on + strand at 3606.602 kb on - strand at 3606.629 kb on - strand at 3606.631 kb on - strand at 3606.636 kb on - strand at 3606.667 kb on + strand at 3606.713 kb on - strand at 3606.713 kb on - strand at 3606.723 kb on - strand at 3606.723 kb on - strand at 3606.723 kb on - strand at 3606.727 kb on + strand at 3606.727 kb on + strand at 3606.727 kb on + strand at 3606.735 kb on - strand at 3606.735 kb on - strand at 3606.735 kb on - strand at 3606.736 kb on - strand at 3606.775 kb on - strand at 3606.775 kb on - strand at 3606.987 kb on + strand, within yhhP at 3606.997 kb on + strand at 3607.058 kb on - strand at 3607.059 kb on - strand at 3607.175 kb on - strand at 3607.177 kb on + strand at 3607.177 kb on + strand at 3607.214 kb on - strand at 3607.274 kb on - strand at 3607.274 kb on - strand at 3607.411 kb on - strand, within yhhQ at 3607.435 kb on + strand, within yhhQ at 3607.447 kb on + strand, within yhhQ at 3607.466 kb on + strand, within yhhQ at 3607.466 kb on + strand, within yhhQ at 3607.482 kb on - strand, within yhhQ at 3607.511 kb on - strand, within yhhQ at 3607.542 kb on + strand, within yhhQ at 3607.603 kb on + strand, within yhhQ at 3607.603 kb on + strand, within yhhQ at 3607.660 kb on - strand, within yhhQ at 3607.660 kb on - strand, within yhhQ at 3607.673 kb on + strand, within yhhQ at 3607.725 kb on + strand, within yhhQ at 3607.725 kb on + strand, within yhhQ at 3607.753 kb on + strand, within yhhQ at 3607.753 kb on + strand, within yhhQ at 3607.954 kb on - strand at 3608.011 kb on - strand at 3608.016 kb on + strand at 3608.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 3,605,846 - zntA b3469 0.62 -1.2 3,605,849 + zntA b3469 0.63 +0.1 3,605,870 - zntA b3469 0.63 -2.2 3,605,905 + zntA b3469 0.65 -1.4 3,605,905 + zntA b3469 0.65 -0.4 3,605,909 + zntA b3469 0.65 -1.0 3,605,932 - zntA b3469 0.66 -1.4 3,606,008 - zntA b3469 0.70 +1.1 3,606,008 - zntA b3469 0.70 +0.1 3,606,008 - zntA b3469 0.70 -1.2 3,606,021 + zntA b3469 0.70 -1.6 3,606,069 - zntA b3469 0.73 -1.7 3,606,074 - zntA b3469 0.73 -1.4 3,606,078 + zntA b3469 0.73 -1.7 3,606,100 + zntA b3469 0.74 -0.4 3,606,100 + zntA b3469 0.74 -0.7 3,606,144 + zntA b3469 0.76 -1.9 3,606,198 + zntA b3469 0.78 -2.7 3,606,203 + zntA b3469 0.79 -1.4 3,606,222 - zntA b3469 0.79 -1.4 3,606,298 + zntA b3469 0.83 -2.4 3,606,326 + zntA b3469 0.84 -2.0 3,606,331 + zntA b3469 0.84 -2.3 3,606,350 - zntA b3469 0.85 -1.9 3,606,350 - zntA b3469 0.85 -1.2 3,606,367 - zntA b3469 0.86 -1.7 3,606,367 - zntA b3469 0.86 +1.1 3,606,367 - zntA b3469 0.86 +0.1 3,606,383 - zntA b3469 0.87 -1.7 3,606,383 - zntA b3469 0.87 -0.4 3,606,414 + zntA b3469 0.88 -1.7 3,606,414 + zntA b3469 0.88 -0.4 3,606,448 + zntA b3469 0.90 -1.9 3,606,448 + zntA b3469 0.90 -1.6 3,606,454 + +0.1 3,606,454 + -2.0 3,606,468 + -1.2 3,606,478 + -1.2 3,606,478 + +1.1 3,606,590 + -1.9 3,606,602 - -0.4 3,606,629 - -0.9 3,606,631 - -1.0 3,606,636 - -1.7 3,606,667 + -0.3 3,606,713 - -0.2 3,606,713 - -0.5 3,606,723 - -0.5 3,606,723 - -0.2 3,606,723 - -0.4 3,606,727 + -0.1 3,606,727 + -0.2 3,606,727 + -0.1 3,606,735 - +0.1 3,606,735 - +0.5 3,606,735 - +0.1 3,606,736 - +0.1 3,606,775 - -0.1 3,606,775 - -0.6 3,606,987 + yhhP b3470 0.87 -0.4 3,606,997 + +0.6 3,607,058 - -0.0 3,607,059 - -0.3 3,607,175 - +0.7 3,607,177 + -0.1 3,607,177 + +0.7 3,607,214 - -0.2 3,607,274 - -1.7 3,607,274 - +0.7 3,607,411 - yhhQ b3471 0.26 +0.3 3,607,435 + yhhQ b3471 0.29 +0.3 3,607,447 + yhhQ b3471 0.31 -0.4 3,607,466 + yhhQ b3471 0.34 -0.1 3,607,466 + yhhQ b3471 0.34 +0.2 3,607,482 - yhhQ b3471 0.36 -0.1 3,607,511 - yhhQ b3471 0.41 +0.1 3,607,542 + yhhQ b3471 0.45 +0.0 3,607,603 + yhhQ b3471 0.55 +0.4 3,607,603 + yhhQ b3471 0.55 -0.9 3,607,660 - yhhQ b3471 0.63 +0.6 3,607,660 - yhhQ b3471 0.63 +0.2 3,607,673 + yhhQ b3471 0.65 -0.3 3,607,725 + yhhQ b3471 0.73 +0.3 3,607,725 + yhhQ b3471 0.73 -1.2 3,607,753 + yhhQ b3471 0.77 -0.2 3,607,753 + yhhQ b3471 0.77 -0.2 3,607,954 - -0.6 3,608,011 - -0.4 3,608,016 + -0.8 3,608,016 + -0.2
Or see this region's nucleotide sequence