Experiment: M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1201 and ycgV are separated by 99 nucleotides ycgV and ychF are separated by 768 nucleotides
b1201: b1201 - putative sensor-type regulator (VIMSS), at 1,250,289 to 1,252,208
b1201
b1202: ycgV - predicted adhesin (NCBI), at 1,252,308 to 1,255,175
ycgV
b1203: ychF - translation-associated GTPase (NCBI), at 1,255,944 to 1,257,035
ychF
Position (kb)
1252
1253
1254
1255
1256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1251.383 kb on - strand, within b1201 at 1251.531 kb on + strand, within b1201 at 1251.643 kb on + strand, within b1201 at 1251.709 kb on - strand, within b1201 at 1251.709 kb on - strand, within b1201 at 1252.094 kb on - strand at 1252.136 kb on + strand at 1252.136 kb on + strand at 1252.302 kb on - strand at 1252.335 kb on + strand at 1252.439 kb on - strand at 1252.463 kb on - strand at 1252.463 kb on - strand at 1252.489 kb on - strand at 1252.517 kb on - strand at 1252.526 kb on - strand at 1252.560 kb on + strand at 1252.595 kb on - strand, within ycgV at 1252.595 kb on - strand, within ycgV at 1252.616 kb on - strand, within ycgV at 1252.616 kb on - strand, within ycgV at 1252.688 kb on + strand, within ycgV at 1252.698 kb on - strand, within ycgV at 1252.698 kb on - strand, within ycgV at 1252.705 kb on - strand, within ycgV at 1252.705 kb on - strand, within ycgV at 1252.713 kb on - strand, within ycgV at 1252.797 kb on + strand, within ycgV at 1252.797 kb on + strand, within ycgV at 1252.805 kb on - strand, within ycgV at 1252.870 kb on + strand, within ycgV at 1252.870 kb on + strand, within ycgV at 1252.993 kb on + strand, within ycgV at 1253.027 kb on - strand, within ycgV at 1253.064 kb on + strand, within ycgV at 1253.180 kb on - strand, within ycgV at 1253.283 kb on + strand, within ycgV at 1253.283 kb on + strand, within ycgV at 1253.509 kb on + strand, within ycgV at 1253.519 kb on - strand, within ycgV at 1253.602 kb on + strand, within ycgV at 1253.633 kb on - strand, within ycgV at 1253.641 kb on + strand, within ycgV at 1253.641 kb on + strand, within ycgV at 1253.681 kb on + strand, within ycgV at 1253.937 kb on - strand, within ycgV at 1253.937 kb on - strand, within ycgV at 1254.020 kb on + strand, within ycgV at 1254.231 kb on + strand, within ycgV at 1254.465 kb on + strand, within ycgV at 1254.465 kb on + strand, within ycgV at 1254.484 kb on + strand, within ycgV at 1254.489 kb on + strand, within ycgV at 1254.612 kb on - strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand, within ycgV at 1254.626 kb on + strand at 1254.634 kb on - strand, within ycgV at 1254.665 kb on + strand, within ycgV at 1254.679 kb on - strand, within ycgV at 1254.681 kb on + strand, within ycgV at 1254.734 kb on + strand, within ycgV at 1254.734 kb on + strand, within ycgV at 1254.788 kb on + strand, within ycgV at 1254.791 kb on - strand, within ycgV at 1254.791 kb on - strand, within ycgV at 1254.809 kb on + strand, within ycgV at 1254.822 kb on - strand, within ycgV at 1254.843 kb on + strand, within ycgV at 1254.872 kb on + strand, within ycgV at 1254.878 kb on + strand, within ycgV at 1254.879 kb on + strand, within ycgV at 1254.900 kb on - strand at 1254.900 kb on - strand at 1254.931 kb on + strand at 1254.934 kb on + strand at 1254.938 kb on + strand at 1254.938 kb on + strand at 1254.938 kb on + strand at 1254.941 kb on - strand at 1254.943 kb on + strand at 1254.948 kb on + strand at 1254.951 kb on - strand at 1254.953 kb on + strand at 1255.026 kb on + strand at 1255.027 kb on + strand at 1255.030 kb on + strand at 1255.039 kb on + strand at 1255.163 kb on + strand at 1255.165 kb on + strand at 1255.175 kb on + strand at 1255.187 kb on + strand at 1255.197 kb on + strand at 1255.582 kb on - strand at 1255.634 kb on + strand at 1255.634 kb on - strand at 1255.634 kb on - strand at 1255.659 kb on + strand at 1255.667 kb on - strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.693 kb on + strand at 1255.713 kb on + strand at 1255.764 kb on + strand at 1255.771 kb on + strand at 1255.799 kb on + strand at 1255.807 kb on - strand at 1255.811 kb on + strand at 1255.811 kb on + strand at 1256.042 kb on - strand at 1256.092 kb on - strand, within ychF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 1,251,383 - b1201 b1201 0.57 -1.3 1,251,531 + b1201 b1201 0.65 -2.4 1,251,643 + b1201 b1201 0.71 -0.2 1,251,709 - b1201 b1201 0.74 +1.1 1,251,709 - b1201 b1201 0.74 -0.5 1,252,094 - -0.5 1,252,136 + -1.1 1,252,136 + +0.7 1,252,302 - -0.1 1,252,335 + -0.2 1,252,439 - +0.1 1,252,463 - -0.0 1,252,463 - -0.2 1,252,489 - +0.1 1,252,517 - -0.1 1,252,526 - +0.6 1,252,560 + -2.8 1,252,595 - ycgV b1202 0.10 +0.6 1,252,595 - ycgV b1202 0.10 +0.2 1,252,616 - ycgV b1202 0.11 -0.5 1,252,616 - ycgV b1202 0.11 -0.2 1,252,688 + ycgV b1202 0.13 -0.4 1,252,698 - ycgV b1202 0.14 +0.5 1,252,698 - ycgV b1202 0.14 +0.2 1,252,705 - ycgV b1202 0.14 -0.6 1,252,705 - ycgV b1202 0.14 +0.1 1,252,713 - ycgV b1202 0.14 -0.0 1,252,797 + ycgV b1202 0.17 -0.6 1,252,797 + ycgV b1202 0.17 +0.5 1,252,805 - ycgV b1202 0.17 +0.5 1,252,870 + ycgV b1202 0.20 +0.5 1,252,870 + ycgV b1202 0.20 +1.1 1,252,993 + ycgV b1202 0.24 -0.4 1,253,027 - ycgV b1202 0.25 -0.0 1,253,064 + ycgV b1202 0.26 -0.7 1,253,180 - ycgV b1202 0.30 +0.4 1,253,283 + ycgV b1202 0.34 +0.0 1,253,283 + ycgV b1202 0.34 +0.0 1,253,509 + ycgV b1202 0.42 +2.4 1,253,519 - ycgV b1202 0.42 -0.0 1,253,602 + ycgV b1202 0.45 +0.3 1,253,633 - ycgV b1202 0.46 +0.5 1,253,641 + ycgV b1202 0.46 -0.0 1,253,641 + ycgV b1202 0.46 -0.2 1,253,681 + ycgV b1202 0.48 -0.1 1,253,937 - ycgV b1202 0.57 -0.1 1,253,937 - ycgV b1202 0.57 +0.6 1,254,020 + ycgV b1202 0.60 -0.7 1,254,231 + ycgV b1202 0.67 +0.4 1,254,465 + ycgV b1202 0.75 -0.5 1,254,465 + ycgV b1202 0.75 +0.4 1,254,484 + ycgV b1202 0.76 +0.1 1,254,489 + ycgV b1202 0.76 +2.9 1,254,612 - ycgV b1202 0.80 -1.8 1,254,626 + ycgV b1202 0.81 +0.1 1,254,626 + ycgV b1202 0.81 +0.4 1,254,626 + ycgV b1202 0.81 -0.4 1,254,626 + ycgV b1202 0.81 -1.2 1,254,626 + ycgV b1202 0.81 +0.2 1,254,626 + ycgV b1202 0.81 -0.2 1,254,626 + ycgV b1202 0.81 -1.2 1,254,626 + +0.4 1,254,634 - ycgV b1202 0.81 -0.0 1,254,665 + ycgV b1202 0.82 -0.2 1,254,679 - ycgV b1202 0.83 -0.5 1,254,681 + ycgV b1202 0.83 +0.2 1,254,734 + ycgV b1202 0.85 -1.7 1,254,734 + ycgV b1202 0.85 +0.5 1,254,788 + ycgV b1202 0.86 -0.1 1,254,791 - ycgV b1202 0.87 -0.5 1,254,791 - ycgV b1202 0.87 +1.1 1,254,809 + ycgV b1202 0.87 -0.2 1,254,822 - ycgV b1202 0.88 +1.1 1,254,843 + ycgV b1202 0.88 -0.3 1,254,872 + ycgV b1202 0.89 -3.0 1,254,878 + ycgV b1202 0.90 +1.1 1,254,879 + ycgV b1202 0.90 +0.1 1,254,900 - +1.1 1,254,900 - +1.1 1,254,931 + +1.5 1,254,934 + +1.5 1,254,938 + -0.9 1,254,938 + +0.3 1,254,938 + +0.5 1,254,941 - +1.1 1,254,943 + -0.2 1,254,948 + +0.7 1,254,951 - +1.1 1,254,953 + +2.0 1,255,026 + +0.1 1,255,027 + +1.1 1,255,030 + +0.6 1,255,039 + +0.1 1,255,163 + +1.1 1,255,165 + +1.1 1,255,175 + +1.1 1,255,187 + +1.1 1,255,197 + +1.1 1,255,582 - -0.2 1,255,634 + +3.1 1,255,634 - +1.1 1,255,634 - +1.1 1,255,659 + +0.5 1,255,667 - +1.1 1,255,693 + -0.5 1,255,693 + +0.5 1,255,693 + -0.6 1,255,693 + +0.1 1,255,693 + +0.1 1,255,693 + +1.5 1,255,693 + +0.1 1,255,693 + +1.1 1,255,693 + +0.4 1,255,713 + -0.7 1,255,764 + -0.5 1,255,771 + +0.4 1,255,799 + +0.1 1,255,807 - +1.1 1,255,811 + +1.1 1,255,811 + +1.1 1,256,042 - +0.5 1,256,092 - ychF b1203 0.14 +1.1
Or see this region's nucleotide sequence