Experiment: M9 Aerobic Capecitabine 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutH and ygdQ are separated by 68 nucleotides ygdQ and ygdR are separated by 137 nucleotides ygdR and tas are separated by 107 nucleotides
b2831: mutH - DNA mismatch repair protein (NCBI), at 2,967,684 to 2,968,373
mutH
b2832: ygdQ - predicted inner membrane protein (NCBI), at 2,968,442 to 2,969,155
ygdQ
b2833: ygdR - hypothetical protein (NCBI), at 2,969,293 to 2,969,511
ygdR
b2834: tas - predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase (NCBI), at 2,969,619 to 2,970,659
tas
Position (kb)
2968
2969
2970 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2967.640 kb on - strand at 2967.694 kb on - strand at 2967.728 kb on + strand at 2967.768 kb on - strand, within mutH at 2967.768 kb on - strand, within mutH at 2967.994 kb on - strand, within mutH at 2968.044 kb on + strand, within mutH at 2968.088 kb on + strand, within mutH at 2968.088 kb on + strand, within mutH at 2968.096 kb on - strand, within mutH at 2968.174 kb on + strand, within mutH at 2968.179 kb on - strand, within mutH at 2968.183 kb on - strand, within mutH at 2968.499 kb on + strand at 2968.509 kb on - strand at 2968.509 kb on - strand at 2968.614 kb on - strand, within ygdQ at 2968.614 kb on - strand, within ygdQ at 2968.620 kb on - strand, within ygdQ at 2968.620 kb on - strand, within ygdQ at 2968.648 kb on + strand, within ygdQ at 2968.648 kb on + strand, within ygdQ at 2968.674 kb on + strand, within ygdQ at 2968.786 kb on - strand, within ygdQ at 2968.829 kb on + strand, within ygdQ at 2968.856 kb on - strand, within ygdQ at 2968.880 kb on + strand, within ygdQ at 2968.942 kb on + strand, within ygdQ at 2968.942 kb on + strand, within ygdQ at 2968.955 kb on - strand, within ygdQ at 2969.039 kb on - strand, within ygdQ at 2969.039 kb on - strand, within ygdQ at 2969.108 kb on - strand at 2969.108 kb on - strand at 2969.160 kb on - strand at 2969.160 kb on - strand at 2969.164 kb on + strand at 2969.172 kb on - strand at 2969.172 kb on - strand at 2969.193 kb on + strand at 2969.193 kb on - strand at 2969.202 kb on - strand at 2969.231 kb on + strand at 2969.242 kb on - strand at 2969.263 kb on + strand at 2969.263 kb on + strand at 2969.280 kb on - strand at 2969.281 kb on - strand at 2969.286 kb on - strand at 2969.327 kb on + strand, within ygdR at 2969.426 kb on + strand, within ygdR at 2969.439 kb on + strand, within ygdR at 2969.439 kb on + strand, within ygdR at 2969.454 kb on + strand, within ygdR at 2969.457 kb on + strand, within ygdR at 2969.457 kb on + strand, within ygdR at 2969.457 kb on + strand, within ygdR at 2969.458 kb on - strand, within ygdR at 2969.467 kb on - strand, within ygdR at 2969.616 kb on + strand at 2969.636 kb on + strand at 2969.642 kb on - strand at 2969.654 kb on + strand at 2969.693 kb on + strand at 2969.699 kb on - strand at 2969.746 kb on + strand, within tas at 2969.746 kb on + strand, within tas at 2969.753 kb on - strand, within tas at 2969.755 kb on - strand, within tas at 2969.872 kb on + strand, within tas at 2969.872 kb on + strand, within tas at 2969.912 kb on - strand, within tas at 2969.917 kb on + strand, within tas at 2969.976 kb on - strand, within tas at 2969.976 kb on - strand, within tas at 2969.984 kb on - strand, within tas at 2969.993 kb on + strand, within tas
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Aerobic Capecitabine 5000 uM remove 2,967,640 - +0.5 2,967,694 - +0.9 2,967,728 + -0.8 2,967,768 - mutH b2831 0.12 -1.3 2,967,768 - mutH b2831 0.12 +0.0 2,967,994 - mutH b2831 0.45 -0.9 2,968,044 + mutH b2831 0.52 -0.3 2,968,088 + mutH b2831 0.59 -0.5 2,968,088 + mutH b2831 0.59 -0.1 2,968,096 - mutH b2831 0.60 -0.7 2,968,174 + mutH b2831 0.71 -0.3 2,968,179 - mutH b2831 0.72 -1.1 2,968,183 - mutH b2831 0.72 +0.0 2,968,499 + -1.0 2,968,509 - -1.1 2,968,509 - -1.0 2,968,614 - ygdQ b2832 0.24 -0.5 2,968,614 - ygdQ b2832 0.24 -1.2 2,968,620 - ygdQ b2832 0.25 -1.3 2,968,620 - ygdQ b2832 0.25 -3.2 2,968,648 + ygdQ b2832 0.29 -2.0 2,968,648 + ygdQ b2832 0.29 -1.0 2,968,674 + ygdQ b2832 0.32 -0.8 2,968,786 - ygdQ b2832 0.48 -2.1 2,968,829 + ygdQ b2832 0.54 -0.9 2,968,856 - ygdQ b2832 0.58 -0.5 2,968,880 + ygdQ b2832 0.61 -1.0 2,968,942 + ygdQ b2832 0.70 -0.6 2,968,942 + ygdQ b2832 0.70 -2.5 2,968,955 - ygdQ b2832 0.72 -0.4 2,969,039 - ygdQ b2832 0.84 -1.0 2,969,039 - ygdQ b2832 0.84 -0.3 2,969,108 - -1.7 2,969,108 - -0.1 2,969,160 - -0.4 2,969,160 - -1.1 2,969,164 + +0.3 2,969,172 - +0.1 2,969,172 - -0.1 2,969,193 + -0.4 2,969,193 - -0.1 2,969,202 - -0.1 2,969,231 + -0.1 2,969,242 - -0.5 2,969,263 + +0.2 2,969,263 + -0.8 2,969,280 - -0.1 2,969,281 - -0.2 2,969,286 - -0.5 2,969,327 + ygdR b2833 0.16 +0.2 2,969,426 + ygdR b2833 0.61 -0.4 2,969,439 + ygdR b2833 0.67 -0.0 2,969,439 + ygdR b2833 0.67 +0.2 2,969,454 + ygdR b2833 0.74 +0.1 2,969,457 + ygdR b2833 0.75 -1.5 2,969,457 + ygdR b2833 0.75 -0.1 2,969,457 + ygdR b2833 0.75 -0.2 2,969,458 - ygdR b2833 0.75 +0.6 2,969,467 - ygdR b2833 0.79 -1.0 2,969,616 + -0.1 2,969,636 + -0.0 2,969,642 - +0.1 2,969,654 + -0.1 2,969,693 + -0.8 2,969,699 - +0.6 2,969,746 + tas b2834 0.12 -0.3 2,969,746 + tas b2834 0.12 +0.4 2,969,753 - tas b2834 0.13 +0.2 2,969,755 - tas b2834 0.13 -0.1 2,969,872 + tas b2834 0.24 +0.0 2,969,872 + tas b2834 0.24 +0.2 2,969,912 - tas b2834 0.28 -0.3 2,969,917 + tas b2834 0.29 +0.7 2,969,976 - tas b2834 0.34 -1.0 2,969,976 - tas b2834 0.34 +0.3 2,969,984 - tas b2834 0.35 -1.5 2,969,993 + tas b2834 0.36 +0.1
Or see this region's nucleotide sequence