Strain Fitness in Escherichia coli BW25113 around b2063

Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwza and yegH are separated by 658 nucleotidesyegH and asmA are separated by 273 nucleotides b2062: wza - lipoprotein required for capsular polysaccharide translocation through the outer membrane (NCBI), at 2,134,128 to 2,135,267 wza b2063: yegH - putative transport protein (VIMSS), at 2,135,926 to 2,137,509 yegH b2064: asmA - predicted assembly protein (NCBI), at 2,137,783 to 2,139,636 asmA Position (kb) 2135 2136 2137 2138Strain fitness (log2 ratio) -2 -1 0 1at 2134.961 kb on + strand, within wzaat 2134.991 kb on - strand, within wzaat 2134.993 kb on - strand, within wzaat 2134.993 kb on - strand, within wzaat 2135.044 kb on + strand, within wzaat 2135.052 kb on - strand, within wzaat 2135.052 kb on - strand, within wzaat 2135.055 kb on - strand, within wzaat 2135.055 kb on - strand, within wzaat 2135.058 kb on + strand, within wzaat 2135.072 kb on - strand, within wzaat 2135.075 kb on + strand, within wzaat 2135.082 kb on - strand, within wzaat 2135.082 kb on - strand, within wzaat 2135.093 kb on + strand, within wzaat 2135.106 kb on + strand, within wzaat 2135.114 kb on - strand, within wzaat 2135.116 kb on - strand, within wzaat 2135.137 kb on + strand, within wzaat 2135.176 kb on + strandat 2135.190 kb on + strandat 2135.193 kb on + strandat 2135.195 kb on + strandat 2135.195 kb on + strandat 2135.219 kb on + strandat 2135.421 kb on - strandat 2135.441 kb on - strandat 2135.450 kb on + strandat 2135.904 kb on - strandat 2135.920 kb on + strandat 2135.932 kb on - strandat 2135.944 kb on + strandat 2135.945 kb on + strandat 2135.945 kb on + strandat 2135.954 kb on + strandat 2136.074 kb on - strandat 2136.150 kb on + strand, within yegHat 2136.150 kb on + strand, within yegHat 2136.150 kb on + strand, within yegHat 2136.257 kb on + strand, within yegHat 2136.257 kb on + strand, within yegHat 2136.363 kb on - strand, within yegHat 2136.836 kb on + strand, within yegHat 2136.865 kb on - strand, within yegHat 2136.940 kb on + strand, within yegHat 2137.000 kb on - strand, within yegHat 2137.000 kb on - strand, within yegHat 2137.023 kb on - strand, within yegHat 2137.147 kb on + strand, within yegHat 2137.264 kb on - strand, within yegHat 2137.290 kb on - strand, within yegHat 2137.305 kb on - strand, within yegHat 2137.305 kb on - strand, within yegHat 2137.355 kb on + strandat 2137.368 kb on + strandat 2137.451 kb on - strandat 2137.471 kb on - strandat 2137.617 kb on - strandat 2137.618 kb on + strandat 2137.618 kb on + strandat 2137.653 kb on - strandat 2137.688 kb on + strandat 2137.764 kb on + strandat 2137.767 kb on - strandat 2137.789 kb on - strandat 2137.844 kb on + strandat 2137.881 kb on - strandat 2137.881 kb on - strandat 2137.934 kb on - strandat 2137.934 kb on - strandat 2137.937 kb on + strandat 2137.944 kb on - strandat 2137.953 kb on + strandat 2137.953 kb on + strandat 2138.077 kb on - strand, within asmAat 2138.115 kb on - strand, within asmAat 2138.212 kb on + strand, within asmAat 2138.230 kb on - strand, within asmAat 2138.243 kb on - strand, within asmAat 2138.328 kb on - strand, within asmAat 2138.328 kb on - strand, within asmAat 2138.347 kb on - strand, within asmAat 2138.402 kb on - strand, within asmAat 2138.402 kb on - strand, within asmA

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
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2,134,961 + wza b2062 0.73 -0.8
2,134,991 - wza b2062 0.76 -0.3
2,134,993 - wza b2062 0.76 +0.4
2,134,993 - wza b2062 0.76 -0.3
2,135,044 + wza b2062 0.80 +1.4
2,135,052 - wza b2062 0.81 -1.3
2,135,052 - wza b2062 0.81 +1.7
2,135,055 - wza b2062 0.81 -0.4
2,135,055 - wza b2062 0.81 +0.3
2,135,058 + wza b2062 0.82 +0.8
2,135,072 - wza b2062 0.83 +0.2
2,135,075 + wza b2062 0.83 +0.6
2,135,082 - wza b2062 0.84 -0.9
2,135,082 - wza b2062 0.84 +0.6
2,135,093 + wza b2062 0.85 +0.3
2,135,106 + wza b2062 0.86 -0.8
2,135,114 - wza b2062 0.86 -1.7
2,135,116 - wza b2062 0.87 -0.6
2,135,137 + wza b2062 0.89 +1.1
2,135,176 + -0.2
2,135,190 + -0.6
2,135,193 + +1.1
2,135,195 + -0.4
2,135,195 + -0.7
2,135,219 + +0.1
2,135,421 - +1.1
2,135,441 - +1.1
2,135,450 + +1.1
2,135,904 - -1.7
2,135,920 + -0.1
2,135,932 - +0.0
2,135,944 + -1.1
2,135,945 + +1.3
2,135,945 + -0.9
2,135,954 + +0.0
2,136,074 - -0.0
2,136,150 + yegH b2063 0.14 +0.5
2,136,150 + yegH b2063 0.14 +0.2
2,136,150 + yegH b2063 0.14 +0.8
2,136,257 + yegH b2063 0.21 +0.6
2,136,257 + yegH b2063 0.21 +0.2
2,136,363 - yegH b2063 0.28 +0.1
2,136,836 + yegH b2063 0.57 +0.3
2,136,865 - yegH b2063 0.59 +0.4
2,136,940 + yegH b2063 0.64 +0.6
2,137,000 - yegH b2063 0.68 -0.0
2,137,000 - yegH b2063 0.68 +0.1
2,137,023 - yegH b2063 0.69 +0.8
2,137,147 + yegH b2063 0.77 +0.0
2,137,264 - yegH b2063 0.84 -0.3
2,137,290 - yegH b2063 0.86 -0.0
2,137,305 - yegH b2063 0.87 +0.4
2,137,305 - yegH b2063 0.87 +0.6
2,137,355 + +0.2
2,137,368 + -0.4
2,137,451 - +0.1
2,137,471 - +0.0
2,137,617 - -0.9
2,137,618 + -0.4
2,137,618 + -0.9
2,137,653 - +0.0
2,137,688 + -0.5
2,137,764 + -0.1
2,137,767 - -0.0
2,137,789 - +0.3
2,137,844 + +0.4
2,137,881 - -1.7
2,137,881 - -0.8
2,137,934 - -0.4
2,137,934 - -0.5
2,137,937 + +0.3
2,137,944 - +0.3
2,137,953 + +1.4
2,137,953 + -0.7
2,138,077 - asmA b2064 0.16 +0.3
2,138,115 - asmA b2064 0.18 -0.0
2,138,212 + asmA b2064 0.23 -0.5
2,138,230 - asmA b2064 0.24 -0.2
2,138,243 - asmA b2064 0.25 -0.7
2,138,328 - asmA b2064 0.29 -1.8
2,138,328 - asmA b2064 0.29 -0.9
2,138,347 - asmA b2064 0.30 -0.1
2,138,402 - asmA b2064 0.33 -1.1
2,138,402 - asmA b2064 0.33 +0.3

Or see this region's nucleotide sequence