Strain Fitness in Escherichia coli BW25113 around b1791

Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeaL and yeaM overlap by 44 nucleotidesyeaM and yeaN are separated by 96 nucleotidesyeaN and yeaO are separated by 54 nucleotidesyeaO and yoaF are separated by 21 nucleotidesyoaF and yeaP are separated by 182 nucleotides b1789: yeaL - conserved inner membrane protein (NCBI), at 1,872,376 to 1,872,822 yeaL b1790: yeaM - predicted DNA-binding transcriptional regulator (NCBI), at 1,872,779 to 1,873,600 yeaM b1791: yeaN - predicted transporter (NCBI), at 1,873,697 to 1,874,878 yeaN b1792: yeaO - orf, hypothetical protein (VIMSS), at 1,874,933 to 1,875,280 yeaO b1793: yoaF - conserved outer membrane protein (NCBI), at 1,875,302 to 1,875,556 yoaF b1794: yeaP - orf, hypothetical protein (VIMSS), at 1,875,739 to 1,876,764 yeaP Position (kb) 1873 1874 1875Strain fitness (log2 ratio) -2 -1 0 1at 1873.148 kb on + strand, within yeaMat 1873.370 kb on + strand, within yeaMat 1873.391 kb on - strand, within yeaMat 1873.472 kb on + strand, within yeaMat 1873.472 kb on + strand, within yeaMat 1873.510 kb on + strand, within yeaMat 1873.559 kb on - strandat 1873.744 kb on - strandat 1873.791 kb on + strandat 1873.799 kb on - strandat 1873.839 kb on - strand, within yeaNat 1873.888 kb on + strand, within yeaNat 1873.888 kb on + strand, within yeaNat 1874.141 kb on - strand, within yeaNat 1874.429 kb on - strand, within yeaNat 1874.429 kb on - strand, within yeaNat 1874.429 kb on - strand, within yeaNat 1874.429 kb on - strand, within yeaNat 1874.457 kb on - strand, within yeaNat 1874.518 kb on - strand, within yeaNat 1874.614 kb on + strand, within yeaNat 1874.783 kb on + strandat 1875.211 kb on + strand, within yeaOat 1875.211 kb on + strand, within yeaOat 1875.211 kb on + strand, within yeaOat 1875.241 kb on - strand, within yeaOat 1875.241 kb on - strand, within yeaOat 1875.291 kb on + strandat 1875.416 kb on - strand, within yoaFat 1875.530 kb on + strand, within yoaFat 1875.571 kb on + strandat 1875.582 kb on + strandat 1875.724 kb on + strandat 1875.848 kb on - strand, within yeaP

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
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1,873,148 + yeaM b1790 0.45 +0.2
1,873,370 + yeaM b1790 0.72 -2.1
1,873,391 - yeaM b1790 0.74 -0.7
1,873,472 + yeaM b1790 0.84 +0.9
1,873,472 + yeaM b1790 0.84 -0.5
1,873,510 + yeaM b1790 0.89 -1.7
1,873,559 - +0.2
1,873,744 - -0.4
1,873,791 + -0.9
1,873,799 - +0.2
1,873,839 - yeaN b1791 0.12 +0.2
1,873,888 + yeaN b1791 0.16 +1.5
1,873,888 + yeaN b1791 0.16 +0.2
1,874,141 - yeaN b1791 0.38 -0.0
1,874,429 - yeaN b1791 0.62 +0.2
1,874,429 - yeaN b1791 0.62 +1.1
1,874,429 - yeaN b1791 0.62 +0.3
1,874,429 - yeaN b1791 0.62 -0.2
1,874,457 - yeaN b1791 0.64 -0.6
1,874,518 - yeaN b1791 0.69 +0.7
1,874,614 + yeaN b1791 0.78 -0.2
1,874,783 + -0.2
1,875,211 + yeaO b1792 0.80 +1.2
1,875,211 + yeaO b1792 0.80 +0.1
1,875,211 + yeaO b1792 0.80 -0.0
1,875,241 - yeaO b1792 0.89 -0.2
1,875,241 - yeaO b1792 0.89 +0.4
1,875,291 + +0.0
1,875,416 - yoaF b1793 0.45 -0.1
1,875,530 + yoaF b1793 0.89 +0.0
1,875,571 + -0.0
1,875,582 + +0.9
1,875,724 + -0.5
1,875,848 - yeaP b1794 0.11 +0.5

Or see this region's nucleotide sequence