Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampG and yajG are separated by 43 nucleotides yajG and bolA are separated by 304 nucleotides bolA and tig are separated by 343 nucleotides
b0433: ampG - muropeptide transporter (NCBI), at 451,294 to 452,769
ampG
b0434: yajG - putative polymerase/proteinase (VIMSS), at 452,813 to 453,391
yajG
b0435: bolA - possible regulator of murein genes (VIMSS), at 453,696 to 454,013
bolA
b0436: tig - trigger factor (NCBI), at 454,357 to 455,655
tig
Position (kb) 453
454
455 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 452.727 kb on - strand at 452.727 kb on - strand at 452.779 kb on - strand at 452.786 kb on + strand at 452.786 kb on + strand at 452.862 kb on - strand at 452.862 kb on - strand at 452.879 kb on + strand, within yajG at 452.879 kb on + strand, within yajG at 452.897 kb on - strand, within yajG at 452.897 kb on - strand, within yajG at 452.935 kb on + strand, within yajG at 452.935 kb on + strand, within yajG at 453.010 kb on - strand, within yajG at 453.010 kb on - strand, within yajG at 453.024 kb on + strand, within yajG at 453.024 kb on + strand, within yajG at 453.026 kb on + strand, within yajG at 453.026 kb on + strand, within yajG at 453.047 kb on + strand, within yajG at 453.047 kb on + strand, within yajG at 453.052 kb on + strand at 453.060 kb on - strand, within yajG at 453.065 kb on - strand, within yajG at 453.065 kb on - strand, within yajG at 453.138 kb on - strand, within yajG at 453.138 kb on - strand, within yajG at 453.198 kb on - strand, within yajG at 453.198 kb on - strand, within yajG at 453.221 kb on + strand, within yajG at 453.221 kb on + strand, within yajG at 453.228 kb on + strand, within yajG at 453.289 kb on - strand, within yajG at 453.289 kb on - strand, within yajG at 453.303 kb on + strand, within yajG at 453.339 kb on - strand at 453.478 kb on - strand at 453.486 kb on - strand at 453.486 kb on - strand at 453.538 kb on + strand at 453.647 kb on + strand at 453.668 kb on + strand at 453.668 kb on + strand at 453.703 kb on + strand at 453.703 kb on + strand at 453.705 kb on - strand at 453.705 kb on - strand at 453.708 kb on - strand at 453.765 kb on + strand, within bolA at 453.794 kb on - strand, within bolA at 453.794 kb on - strand, within bolA at 453.794 kb on - strand, within bolA at 453.832 kb on - strand, within bolA at 453.835 kb on + strand, within bolA at 453.835 kb on + strand, within bolA at 453.929 kb on + strand, within bolA at 453.937 kb on - strand, within bolA at 453.937 kb on - strand, within bolA at 453.939 kb on + strand, within bolA at 453.939 kb on + strand, within bolA at 453.957 kb on + strand, within bolA at 453.996 kb on + strand at 454.039 kb on + strand at 454.128 kb on - strand at 454.177 kb on - strand at 454.177 kb on - strand at 454.207 kb on + strand at 454.264 kb on - strand at 454.299 kb on + strand at 454.299 kb on + strand at 454.302 kb on - strand at 454.334 kb on + strand at 454.334 kb on - strand at 454.334 kb on - strand at 454.386 kb on - strand at 454.387 kb on - strand at 454.452 kb on + strand at 454.452 kb on + strand at 454.452 kb on + strand at 454.460 kb on - strand at 454.460 kb on - strand at 454.543 kb on + strand, within tig at 454.543 kb on + strand, within tig at 454.561 kb on + strand, within tig at 454.578 kb on - strand, within tig at 454.747 kb on + strand, within tig at 454.752 kb on + strand, within tig at 454.776 kb on + strand, within tig at 454.776 kb on + strand, within tig at 454.776 kb on + strand, within tig at 454.924 kb on + strand, within tig at 454.924 kb on + strand, within tig at 454.932 kb on - strand, within tig
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 452,727 - +0.1 452,727 - -0.8 452,779 - +0.1 452,786 + -0.6 452,786 + -0.5 452,862 - +0.0 452,862 - -0.2 452,879 + yajG b0434 0.11 -1.2 452,879 + yajG b0434 0.11 -0.6 452,897 - yajG b0434 0.15 -1.5 452,897 - yajG b0434 0.15 +0.1 452,935 + yajG b0434 0.21 -3.0 452,935 + yajG b0434 0.21 +0.6 453,010 - yajG b0434 0.34 +0.2 453,010 - yajG b0434 0.34 -0.3 453,024 + yajG b0434 0.36 -0.6 453,024 + yajG b0434 0.36 -1.1 453,026 + yajG b0434 0.37 -2.2 453,026 + yajG b0434 0.37 +0.6 453,047 + yajG b0434 0.40 -0.5 453,047 + yajG b0434 0.40 -1.1 453,052 + +0.6 453,060 - yajG b0434 0.43 -0.0 453,065 - yajG b0434 0.44 -2.1 453,065 - yajG b0434 0.44 -0.8 453,138 - yajG b0434 0.56 -0.2 453,138 - yajG b0434 0.56 -0.4 453,198 - yajG b0434 0.66 +0.1 453,198 - yajG b0434 0.66 -0.1 453,221 + yajG b0434 0.70 -1.7 453,221 + yajG b0434 0.70 -1.9 453,228 + yajG b0434 0.72 -1.9 453,289 - yajG b0434 0.82 +0.1 453,289 - yajG b0434 0.82 -0.3 453,303 + yajG b0434 0.85 -2.4 453,339 - +0.9 453,478 - -1.2 453,486 - -0.0 453,486 - -0.5 453,538 + +1.2 453,647 + +0.2 453,668 + -1.0 453,668 + +0.2 453,703 + -0.6 453,703 + +0.2 453,705 - +0.3 453,705 - +0.6 453,708 - +1.0 453,765 + bolA b0435 0.22 +0.1 453,794 - bolA b0435 0.31 +0.7 453,794 - bolA b0435 0.31 -0.2 453,794 - bolA b0435 0.31 -0.2 453,832 - bolA b0435 0.43 +0.8 453,835 + bolA b0435 0.44 +0.6 453,835 + bolA b0435 0.44 +0.5 453,929 + bolA b0435 0.73 +0.6 453,937 - bolA b0435 0.76 +0.3 453,937 - bolA b0435 0.76 +0.2 453,939 + bolA b0435 0.76 -0.1 453,939 + bolA b0435 0.76 +0.9 453,957 + bolA b0435 0.82 +0.6 453,996 + +0.6 454,039 + +0.4 454,128 - -0.5 454,177 - +0.5 454,177 - +0.3 454,207 + +0.0 454,264 - -0.5 454,299 + -0.3 454,299 + -0.3 454,302 - -0.4 454,334 + -0.5 454,334 - -2.1 454,334 - -0.5 454,386 - -0.3 454,387 - -0.8 454,452 + -0.0 454,452 + -2.1 454,452 + +0.2 454,460 - -0.4 454,460 - -1.5 454,543 + tig b0436 0.14 -0.7 454,543 + tig b0436 0.14 -1.6 454,561 + tig b0436 0.16 -1.9 454,578 - tig b0436 0.17 -2.1 454,747 + tig b0436 0.30 -1.5 454,752 + tig b0436 0.30 -1.0 454,776 + tig b0436 0.32 -1.3 454,776 + tig b0436 0.32 -1.3 454,776 + tig b0436 0.32 -1.6 454,924 + tig b0436 0.44 -2.0 454,924 + tig b0436 0.44 -1.8 454,932 - tig b0436 0.44 +0.8
Or see this region's nucleotide sequence