Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagX and yagY are separated by 25 nucleotides yagY and yagZ are separated by 57 nucleotides yagZ and ykgK are separated by 74 nucleotides
b0291: yagX - predicted aromatic compound dioxygenase (NCBI), at 306,031 to 308,556
yagX
b0292: yagY - hypothetical protein (NCBI), at 308,582 to 309,250
yagY
b0293: yagZ - hypothetical protein (NCBI), at 309,308 to 309,895
yagZ
b0294: ykgK - predicted regulator (NCBI), at 309,970 to 310,560
ykgK
Position (kb) 308
309
310 Strain fitness (log2 ratio) -1
0
1 at 307.645 kb on - strand, within yagX at 307.645 kb on - strand, within yagX at 307.658 kb on - strand, within yagX at 307.662 kb on + strand, within yagX at 307.662 kb on + strand, within yagX at 307.663 kb on - strand, within yagX at 307.770 kb on + strand, within yagX at 307.911 kb on - strand, within yagX at 307.911 kb on - strand, within yagX at 307.958 kb on - strand, within yagX at 308.053 kb on - strand, within yagX at 308.102 kb on - strand, within yagX at 308.102 kb on - strand, within yagX at 308.218 kb on - strand, within yagX at 308.218 kb on - strand, within yagX at 308.266 kb on - strand, within yagX at 308.376 kb on + strand at 308.381 kb on - strand at 308.381 kb on - strand at 308.457 kb on + strand at 308.529 kb on + strand at 308.529 kb on + strand at 308.585 kb on - strand at 308.711 kb on - strand, within yagY at 308.739 kb on - strand, within yagY at 308.807 kb on + strand, within yagY at 308.835 kb on + strand, within yagY at 308.835 kb on + strand, within yagY at 308.957 kb on + strand, within yagY at 308.957 kb on + strand, within yagY at 308.957 kb on + strand, within yagY at 308.984 kb on + strand, within yagY at 309.053 kb on - strand, within yagY at 309.149 kb on + strand, within yagY at 309.158 kb on - strand, within yagY at 309.166 kb on - strand, within yagY at 309.300 kb on - strand at 309.312 kb on - strand at 309.370 kb on - strand, within yagZ at 309.370 kb on - strand, within yagZ at 309.401 kb on - strand, within yagZ at 309.401 kb on - strand, within yagZ at 309.435 kb on - strand, within yagZ at 309.515 kb on - strand, within yagZ at 309.532 kb on - strand, within yagZ at 309.552 kb on + strand, within yagZ at 309.583 kb on - strand, within yagZ at 309.597 kb on + strand, within yagZ at 309.609 kb on + strand, within yagZ at 309.609 kb on + strand, within yagZ at 309.630 kb on + strand, within yagZ at 309.640 kb on + strand, within yagZ at 309.657 kb on + strand, within yagZ at 309.673 kb on - strand, within yagZ at 309.726 kb on + strand, within yagZ at 309.778 kb on + strand, within yagZ at 309.778 kb on + strand, within yagZ at 309.800 kb on + strand, within yagZ at 309.804 kb on + strand, within yagZ at 309.819 kb on + strand, within yagZ at 309.822 kb on - strand, within yagZ at 309.839 kb on - strand at 309.839 kb on - strand at 309.841 kb on + strand at 309.847 kb on + strand at 309.870 kb on + strand at 309.934 kb on - strand at 309.938 kb on - strand at 309.961 kb on + strand at 309.961 kb on + strand at 310.001 kb on + strand at 310.001 kb on + strand at 310.006 kb on + strand at 310.013 kb on + strand at 310.013 kb on + strand at 310.013 kb on + strand at 310.021 kb on - strand at 310.028 kb on + strand at 310.029 kb on - strand, within ykgK at 310.033 kb on - strand, within ykgK at 310.035 kb on - strand, within ykgK at 310.035 kb on - strand, within ykgK at 310.035 kb on - strand, within ykgK at 310.035 kb on - strand, within ykgK at 310.044 kb on - strand, within ykgK at 310.044 kb on - strand, within ykgK at 310.102 kb on + strand, within ykgK at 310.219 kb on - strand, within ykgK at 310.219 kb on - strand, within ykgK
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 307,645 - yagX b0291 0.64 -0.2 307,645 - yagX b0291 0.64 +0.1 307,658 - yagX b0291 0.64 -0.2 307,662 + yagX b0291 0.65 -0.0 307,662 + yagX b0291 0.65 -0.9 307,663 - yagX b0291 0.65 -0.7 307,770 + yagX b0291 0.69 +0.4 307,911 - yagX b0291 0.74 +0.0 307,911 - yagX b0291 0.74 +0.3 307,958 - yagX b0291 0.76 -0.0 308,053 - yagX b0291 0.80 -0.4 308,102 - yagX b0291 0.82 -0.3 308,102 - yagX b0291 0.82 -0.0 308,218 - yagX b0291 0.87 +0.7 308,218 - yagX b0291 0.87 -0.1 308,266 - yagX b0291 0.88 +0.5 308,376 + -0.1 308,381 - +1.2 308,381 - -0.2 308,457 + -0.2 308,529 + +0.1 308,529 + +0.2 308,585 - +0.1 308,711 - yagY b0292 0.19 -0.3 308,739 - yagY b0292 0.23 -0.3 308,807 + yagY b0292 0.34 +0.1 308,835 + yagY b0292 0.38 +0.2 308,835 + yagY b0292 0.38 +0.0 308,957 + yagY b0292 0.56 +0.0 308,957 + yagY b0292 0.56 +1.0 308,957 + yagY b0292 0.56 +0.5 308,984 + yagY b0292 0.60 +0.4 309,053 - yagY b0292 0.70 +0.0 309,149 + yagY b0292 0.85 +0.5 309,158 - yagY b0292 0.86 -0.1 309,166 - yagY b0292 0.87 +0.7 309,300 - -0.6 309,312 - -0.1 309,370 - yagZ b0293 0.11 -0.2 309,370 - yagZ b0293 0.11 -0.3 309,401 - yagZ b0293 0.16 +0.6 309,401 - yagZ b0293 0.16 -0.2 309,435 - yagZ b0293 0.22 -0.1 309,515 - yagZ b0293 0.35 -0.4 309,532 - yagZ b0293 0.38 +0.2 309,552 + yagZ b0293 0.41 +0.7 309,583 - yagZ b0293 0.47 -0.6 309,597 + yagZ b0293 0.49 -0.5 309,609 + yagZ b0293 0.51 -0.3 309,609 + yagZ b0293 0.51 +0.1 309,630 + yagZ b0293 0.55 -0.7 309,640 + yagZ b0293 0.56 -0.7 309,657 + yagZ b0293 0.59 -0.3 309,673 - yagZ b0293 0.62 -1.4 309,726 + yagZ b0293 0.71 -0.8 309,778 + yagZ b0293 0.80 +0.9 309,778 + yagZ b0293 0.80 -1.0 309,800 + yagZ b0293 0.84 -0.0 309,804 + yagZ b0293 0.84 +0.1 309,819 + yagZ b0293 0.87 -0.9 309,822 - yagZ b0293 0.87 +0.2 309,839 - -0.0 309,839 - +0.0 309,841 + +1.2 309,847 + +0.2 309,870 + +0.2 309,934 - +0.2 309,938 - +0.2 309,961 + -0.8 309,961 + +0.6 310,001 + +1.2 310,001 + +0.2 310,006 + +1.2 310,013 + +1.2 310,013 + +0.2 310,013 + +1.2 310,021 - +0.5 310,028 + +1.2 310,029 - ykgK b0294 0.10 +0.1 310,033 - ykgK b0294 0.11 -0.4 310,035 - ykgK b0294 0.11 +1.2 310,035 - ykgK b0294 0.11 -1.1 310,035 - ykgK b0294 0.11 -0.8 310,035 - ykgK b0294 0.11 +1.2 310,044 - ykgK b0294 0.13 +0.9 310,044 - ykgK b0294 0.13 +0.2 310,102 + ykgK b0294 0.22 +1.2 310,219 - ykgK b0294 0.42 +1.2 310,219 - ykgK b0294 0.42 +1.2
Or see this region's nucleotide sequence