Strain Fitness in Escherichia coli BW25113 around b3958

Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
4,152,083	-	argE	b3957	0.32	-1.8
4,152,094	-	argE	b3957	0.33	-1.4
4,152,094	-	argE	b3957	0.33	-1.6
4,152,107	+	argE	b3957	0.34	-2.3
4,152,125	-	argE	b3957	0.35	-2.2
4,152,138	+	argE	b3957	0.36	-1.1
4,152,138	+	argE	b3957	0.36	-2.1
4,152,144	+	argE	b3957	0.37	-1.8
4,152,156	+	argE	b3957	0.38	-1.3
4,152,163	+	argE	b3957	0.39	-2.1
4,152,163	+	argE	b3957	0.39	-2.6
4,152,163	-	argE	b3957	0.39	-1.4
4,152,169	-	argE	b3957	0.39	-1.4
4,152,172	+	argE	b3957	0.39	-1.3
4,152,187	-	argE	b3957	0.41	-2.3
4,152,191	-	argE	b3957	0.41	-1.5
4,152,191	-	argE	b3957	0.41	-2.9
4,152,244	+	argE	b3957	0.46	-0.9
4,152,251	-	argE	b3957	0.46	-2.2
4,152,306	-	argE	b3957	0.51	-1.9
4,152,306	-	argE	b3957	0.51	-1.6
4,152,311	-	argE	b3957	0.51	-2.4
4,152,311	-	argE	b3957	0.51	-2.6
4,152,329	+	argE	b3957	0.53	-1.8
4,152,363	+	argE	b3957	0.56	-2.9
4,152,377	+	argE	b3957	0.57	-2.3
4,152,543	+	argE	b3957	0.72	-2.3
4,152,605	-	argE	b3957	0.77	-1.5
4,152,623	+	argE	b3957	0.78	-1.7
4,152,652	+	argE	b3957	0.81	-1.3
4,152,663	+	argE	b3957	0.82	-1.9
4,152,671	-	argE	b3957	0.83	-1.5
4,152,671	-	argE	b3957	0.83	-1.9
4,152,676	-	argE	b3957	0.83	-1.5
4,152,676	-	argE	b3957	0.83	-1.4
4,152,718	-	argE	b3957	0.87	-1.9
4,152,725	-	argE	b3957	0.87	-1.8
4,152,778	+				-0.4
4,152,876	+				+1.0
4,152,881	+				+0.6
4,152,889	-				-1.1
4,152,889	-				-1.8
4,152,952	-				-1.2
4,152,952	-				-1.4
4,153,026	+				-0.4
4,153,072	-				-1.2
4,153,072	-				-2.4
4,153,082	+				-1.1
4,153,082	+				-1.6
4,153,087	-				-2.5
4,153,101	+				-1.6
4,153,137	-	argC	b3958	0.11	-3.3
4,153,184	-	argC	b3958	0.16	-1.4
4,153,208	-	argC	b3958	0.18	-1.3
4,153,208	-	argC	b3958	0.18	-1.5
4,153,240	-	argC	b3958	0.21	-2.9
4,153,240	-	argC	b3958	0.21	-1.7
4,153,244	+	argC	b3958	0.22	-2.4
4,153,261	+	argC	b3958	0.24	-2.3
4,153,298	+	argC	b3958	0.27	+1.1
4,153,326	-	argC	b3958	0.30	-2.1
4,153,348	+	argC	b3958	0.32	-0.9
4,153,384	+	argC	b3958	0.36	-2.2
4,153,384	+	argC	b3958	0.36	-2.7
4,153,387	-	argC	b3958	0.36	-1.8
4,153,390	-	argC	b3958	0.36	-2.5
4,153,424	+	argC	b3958	0.40	-1.8
4,153,425	-	argC	b3958	0.40	-2.5
4,153,425	-	argC	b3958	0.40	-2.9
4,153,440	-	argC	b3958	0.41	-1.5
4,153,442	-	argC	b3958	0.42	-1.1
4,153,501	-	argC	b3958	0.47	-2.3
4,153,586	+	argC	b3958	0.56	-1.6
4,153,586	+	argC	b3958	0.56	-0.6
4,153,631	+	argC	b3958	0.60	-2.2
4,153,638	+	argC	b3958	0.61	-2.3
4,153,647	+	argC	b3958	0.62	-1.9
4,153,647	+	argC	b3958	0.62	-1.8
4,153,656	-	argC	b3958	0.63	-2.0
4,153,656	-	argC	b3958	0.63	-2.6
4,153,676	+	argC	b3958	0.65	-1.1
4,153,684	-	argC	b3958	0.66	-2.1
4,153,684	-				-1.2
4,153,694	-	argC	b3958	0.67	-2.0
4,153,699	-	argC	b3958	0.67	-4.1
4,153,699	-	argC	b3958	0.67	-0.8
4,153,809	+	argC	b3958	0.78	-1.6
4,153,825	-	argC	b3958	0.80	-1.2
4,153,850	-	argC	b3958	0.82	-1.9
4,153,852	-	argC	b3958	0.82	-2.2
4,153,917	+	argC	b3958	0.89	-1.5
4,153,917	+	argC	b3958	0.89	-1.3
4,153,939	+				-1.9
4,153,939	+				-1.9
4,153,972	+				-1.7
4,154,018	+				+0.5
4,154,018	+				+0.3
4,154,023	+				+0.0
4,154,023	+				+0.9
4,154,023	+				-0.1
4,154,074	+				-2.0
4,154,079	+				-2.4
4,154,087	-				-2.7
4,154,090	-				-3.0
4,154,098	-				-1.9
4,154,121	+	argB	b3959	0.11	-1.9
4,154,144	+	argB	b3959	0.14	-1.7
4,154,153	+	argB	b3959	0.15	-2.2
4,154,249	+	argB	b3959	0.27	-1.1
4,154,249	+	argB	b3959	0.27	-2.8
4,154,353	+	argB	b3959	0.41	-0.2
4,154,353	+	argB	b3959	0.41	-2.5
4,154,389	+	argB	b3959	0.45	-1.6
4,154,394	-	argB	b3959	0.46	-2.5
4,154,403	+	argB	b3959	0.47	-1.6
4,154,421	+	argB	b3959	0.50	-1.6
4,154,421	+	argB	b3959	0.50	-1.3
4,154,472	+	argB	b3959	0.56	-0.8
4,154,487	-	argB	b3959	0.58	-1.4
4,154,497	-	argB	b3959	0.59	-1.9
4,154,509	+	argB	b3959	0.61	-2.5
4,154,517	-	argB	b3959	0.62	-2.5
4,154,549	-	argB	b3959	0.66	-2.5
4,154,659	+	argB	b3959	0.80	-1.7
4,154,667	-	argB	b3959	0.81	-2.1
4,154,667	-	argB	b3959	0.81	-1.5
4,154,671	-	argB	b3959	0.82	-1.2
4,154,683	-	argB	b3959	0.83	-2.5
4,154,683	-	argB	b3959	0.83	-2.4
4,154,696	+	argB	b3959	0.85	-2.3
4,154,701	+	argB	b3959	0.86	-2.3
4,154,707	+	argB	b3959	0.86	-1.9
4,154,729	+	argB	b3959	0.89	-1.3
4,154,729	+	argB	b3959	0.89	-1.4
4,154,750	+				-0.5
4,154,750	+				-1.2
4,154,761	+				-1.5
4,154,761	+				-1.7
4,154,763	+				-1.8
4,154,763	+				-1.5
4,154,771	-				-1.9
4,154,773	+				-3.0
4,154,878	-				-2.9
4,154,900	-				-1.4
4,154,901	+				-4.5
4,154,968	+				-5.4
4,155,017	+	argH	b3960	0.10	-2.3
4,155,019	-	argH	b3960	0.11	-2.5