Strain Fitness in Escherichia coli BW25113 around b2499

Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
2,618,356	+	upp	b2498	0.14	+0.8
2,618,361	-	upp	b2498	0.15	+0.5
2,618,361	-	upp	b2498	0.15	+1.2
2,618,361	-	upp	b2498	0.15	+1.0
2,618,364	-	upp	b2498	0.15	+2.1
2,618,402	+	upp	b2498	0.21	-0.5
2,618,561	+	upp	b2498	0.47	+1.1
2,618,648	+	upp	b2498	0.61	+1.3
2,618,648	+	upp	b2498	0.61	+2.1
2,618,729	-	upp	b2498	0.74	+0.6
2,618,770	+	upp	b2498	0.80	+0.3
2,618,778	-	upp	b2498	0.81	+1.7
2,618,846	+				+0.5
2,618,889	-				+0.9
2,619,127	+				-0.1
2,619,127	+				-0.8
2,619,262	+				-3.5
2,619,284	+				-3.8
2,619,362	+	purM	b2499	0.14	-3.3
2,619,501	-	purM	b2499	0.27	-3.5
2,619,662	+	purM	b2499	0.43	-2.1
2,619,662	+	purM	b2499	0.43	-2.4
2,619,832	+	purM	b2499	0.59	-4.1
2,619,832	+	purM	b2499	0.59	-2.8
2,619,835	-	purM	b2499	0.59	-2.4
2,619,923	-	purM	b2499	0.68	-3.4
2,619,923	-	purM	b2499	0.68	-3.2
2,619,971	-	purM	b2499	0.72	-3.5
2,620,035	+	purM	b2499	0.79	-3.8
2,620,035	+	purM	b2499	0.79	-3.6
2,620,043	-	purM	b2499	0.79	-3.2
2,620,164	+				-0.7
2,620,164	+				+0.1
2,620,164	+				-1.0
2,620,175	+				-1.6
2,620,258	+				+1.0
2,620,258	+				+0.2
2,620,260	+				+0.5
2,620,422	-	purN	b2500	0.26	-0.4
2,620,451	+	purN	b2500	0.31	+0.7
2,620,459	-	purN	b2500	0.32	+0.2
2,620,470	-	purN	b2500	0.33	-0.2
2,620,604	+	purN	b2500	0.54	+0.2
2,620,651	+	purN	b2500	0.62	-0.8
2,620,651	+	purN	b2500	0.62	-0.5
2,620,661	-	purN	b2500	0.63	-0.1
2,620,744	+	purN	b2500	0.76	+0.3
2,620,873	+				+0.4
2,620,873	+				+0.4
2,620,899	-				-1.0
2,620,981	+				+0.5
2,620,981	+				+1.0
2,620,989	-				+0.6
2,620,989	-				+0.4
2,620,998	-				+0.5
2,621,024	-				-0.1
2,621,032	-				-0.0
2,621,041	-				-0.2
2,621,118	-				-0.9