Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabG and acpP are separated by 210 nucleotides acpP and fabF are separated by 87 nucleotides fabF and pabC are separated by 119 nucleotides pabC and yceG are separated by 2 nucleotides
b1093: fabG - 3-oxoacyl-[acyl-carrier-protein] reductase (NCBI), at 1,149,893 to 1,150,627
fabG
b1094: acpP - acyl carrier protein (NCBI), at 1,150,838 to 1,151,074
acpP
b1095: fabF - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,151,162 to 1,152,403
fabF
b1096: pabC - 4-amino-4-deoxychorismate lyase (NCBI), at 1,152,523 to 1,153,332
pabC
b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357
yceG
Position (kb)
1151
1152
1153 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1151.238 kb on + strand at 1151.266 kb on + strand at 1151.266 kb on + strand at 1151.368 kb on - strand, within fabF at 1151.416 kb on - strand, within fabF at 1151.427 kb on - strand, within fabF at 1151.427 kb on - strand, within fabF at 1151.530 kb on - strand, within fabF at 1151.530 kb on - strand, within fabF at 1151.535 kb on - strand, within fabF at 1151.535 kb on - strand, within fabF at 1151.608 kb on + strand, within fabF at 1151.608 kb on + strand, within fabF at 1151.610 kb on + strand, within fabF at 1151.697 kb on - strand at 1151.697 kb on - strand, within fabF at 1151.703 kb on - strand, within fabF at 1151.783 kb on + strand, within fabF at 1152.010 kb on - strand, within fabF at 1152.010 kb on - strand, within fabF at 1152.177 kb on - strand, within fabF at 1152.219 kb on + strand, within fabF at 1152.219 kb on + strand, within fabF at 1152.219 kb on + strand, within fabF at 1152.332 kb on + strand at 1152.335 kb on + strand at 1152.361 kb on - strand at 1152.361 kb on - strand at 1152.420 kb on + strand at 1152.420 kb on + strand at 1152.474 kb on - strand at 1152.482 kb on - strand at 1152.520 kb on + strand at 1152.520 kb on + strand at 1152.558 kb on + strand at 1152.676 kb on - strand, within pabC at 1152.788 kb on + strand, within pabC at 1152.788 kb on + strand, within pabC at 1152.876 kb on + strand, within pabC at 1152.902 kb on - strand, within pabC at 1152.909 kb on + strand, within pabC at 1152.909 kb on + strand, within pabC at 1153.012 kb on + strand, within pabC at 1153.171 kb on + strand, within pabC at 1153.177 kb on + strand, within pabC at 1153.208 kb on - strand, within pabC at 1153.223 kb on + strand, within pabC at 1153.301 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 1,151,238 + -2.2 1,151,266 + -2.2 1,151,266 + -1.7 1,151,368 - fabF b1095 0.17 +1.3 1,151,416 - fabF b1095 0.20 -3.6 1,151,427 - fabF b1095 0.21 -2.0 1,151,427 - fabF b1095 0.21 -1.6 1,151,530 - fabF b1095 0.30 -2.6 1,151,530 - fabF b1095 0.30 -1.1 1,151,535 - fabF b1095 0.30 -1.1 1,151,535 - fabF b1095 0.30 -1.3 1,151,608 + fabF b1095 0.36 -2.1 1,151,608 + fabF b1095 0.36 -0.5 1,151,610 + fabF b1095 0.36 -0.9 1,151,697 - -2.8 1,151,697 - fabF b1095 0.43 -1.0 1,151,703 - fabF b1095 0.44 -1.8 1,151,783 + fabF b1095 0.50 -1.2 1,152,010 - fabF b1095 0.68 -2.0 1,152,010 - fabF b1095 0.68 -2.3 1,152,177 - fabF b1095 0.82 -1.6 1,152,219 + fabF b1095 0.85 -0.3 1,152,219 + fabF b1095 0.85 -0.8 1,152,219 + fabF b1095 0.85 -1.1 1,152,332 + -1.4 1,152,335 + -2.5 1,152,361 - -2.1 1,152,361 - -0.6 1,152,420 + -1.3 1,152,420 + -1.7 1,152,474 - -0.2 1,152,482 - +0.0 1,152,520 + +0.3 1,152,520 + +0.2 1,152,558 + -2.4 1,152,676 - pabC b1096 0.19 -0.6 1,152,788 + pabC b1096 0.33 -0.3 1,152,788 + pabC b1096 0.33 +0.2 1,152,876 + pabC b1096 0.44 +0.3 1,152,902 - pabC b1096 0.47 -0.2 1,152,909 + pabC b1096 0.48 +0.2 1,152,909 + pabC b1096 0.48 -0.1 1,153,012 + pabC b1096 0.60 -1.4 1,153,171 + pabC b1096 0.80 -2.3 1,153,177 + pabC b1096 0.81 -0.8 1,153,208 - pabC b1096 0.85 -0.4 1,153,223 + pabC b1096 0.86 -1.1 1,153,301 - -1.0
Or see this region's nucleotide sequence