Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucB and sucC are separated by 274 nucleotides sucC and sucD overlap by 1 nucleotides sucD and mngR are separated by 103 nucleotides
b0727: sucB - dihydrolipoamide acetyltransferase (NCBI), at 760,745 to 761,962
sucB
b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403
sucC
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
Position (kb) 762
763
764 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 761.505 kb on + strand, within sucB at 761.728 kb on + strand, within sucB at 761.860 kb on + strand at 761.970 kb on + strand at 761.992 kb on + strand at 762.001 kb on - strand at 762.146 kb on + strand at 762.154 kb on - strand at 762.154 kb on - strand at 762.196 kb on + strand at 762.223 kb on + strand at 762.284 kb on - strand at 762.308 kb on - strand at 762.310 kb on + strand at 762.318 kb on - strand at 762.398 kb on - strand, within sucC at 762.398 kb on - strand, within sucC at 762.600 kb on - strand, within sucC at 762.708 kb on + strand, within sucC at 762.708 kb on + strand, within sucC at 762.713 kb on + strand, within sucC at 762.989 kb on - strand, within sucC at 762.999 kb on - strand, within sucC at 763.015 kb on + strand, within sucC at 763.070 kb on - strand, within sucC at 763.131 kb on + strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.278 kb on + strand, within sucC at 763.625 kb on + strand, within sucD at 763.625 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.773 kb on + strand, within sucD at 763.871 kb on - strand, within sucD at 763.901 kb on + strand, within sucD at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 761,505 + sucB b0727 0.62 -0.3 761,728 + sucB b0727 0.81 -0.4 761,860 + +0.3 761,970 + -0.2 761,992 + -0.2 762,001 - -0.3 762,146 + -0.5 762,154 - -0.2 762,154 - +0.1 762,196 + -0.9 762,223 + -0.6 762,284 - -2.3 762,308 - -4.4 762,310 + -3.3 762,318 - -3.4 762,398 - sucC b0728 0.14 -3.1 762,398 - sucC b0728 0.14 -4.3 762,600 - sucC b0728 0.31 -0.3 762,708 + sucC b0728 0.40 -1.4 762,708 + sucC b0728 0.40 -1.2 762,713 + sucC b0728 0.41 -3.9 762,989 - sucC b0728 0.64 -2.9 762,999 - sucC b0728 0.65 -1.8 763,015 + sucC b0728 0.67 -1.9 763,070 - sucC b0728 0.71 -1.1 763,131 + sucC b0728 0.77 -2.1 763,206 - sucC b0728 0.83 -2.3 763,206 - sucC b0728 0.83 -2.7 763,206 - sucC b0728 0.83 -1.1 763,278 + sucC b0728 0.89 -3.2 763,625 + sucD b0729 0.26 -0.9 763,625 + sucD b0729 0.26 -2.1 763,712 + sucD b0729 0.36 -1.5 763,712 + sucD b0729 0.36 -3.3 763,773 + sucD b0729 0.43 -2.1 763,871 - sucD b0729 0.54 -4.1 763,901 + sucD b0729 0.57 -0.9 764,252 - -0.9 764,279 + -1.7 764,302 + -1.3 764,302 + +0.1 764,311 + -0.2 764,319 + +0.0 764,349 + +0.2
Or see this region's nucleotide sequence