Experiment: M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queA and tgt are separated by 55 nucleotides tgt and yajC are separated by 22 nucleotides yajC and secD are separated by 27 nucleotides
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
b0406: tgt - queuine tRNA-ribosyltransferase (NCBI), at 425,361 to 426,488
tgt
b0407: yajC - preprotein translocase subunit YajC (NCBI), at 426,511 to 426,843
yajC
b0408: secD - protein export protein SecD (NCBI), at 426,871 to 428,718
secD
Position (kb) 425
426
427 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA at 424.612 kb on + strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.657 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.922 kb on - strand, within queA at 425.147 kb on - strand, within queA at 425.147 kb on - strand, within queA at 425.300 kb on + strand at 425.300 kb on + strand at 425.436 kb on - strand at 425.470 kb on + strand at 425.700 kb on - strand, within tgt at 425.700 kb on - strand, within tgt at 425.852 kb on - strand, within tgt at 425.914 kb on + strand, within tgt at 425.945 kb on - strand, within tgt at 425.945 kb on - strand at 425.964 kb on + strand, within tgt at 426.030 kb on - strand, within tgt at 426.030 kb on - strand, within tgt at 426.035 kb on - strand, within tgt at 426.035 kb on - strand, within tgt at 426.230 kb on + strand, within tgt at 426.230 kb on + strand, within tgt at 426.241 kb on + strand, within tgt at 426.249 kb on - strand, within tgt at 426.259 kb on - strand, within tgt at 426.277 kb on - strand, within tgt at 426.318 kb on - strand, within tgt at 426.352 kb on - strand, within tgt at 426.352 kb on - strand, within tgt at 426.352 kb on - strand, within tgt at 426.352 kb on - strand, within tgt at 426.405 kb on + strand at 426.481 kb on - strand at 426.561 kb on + strand, within yajC at 426.666 kb on + strand, within yajC at 426.666 kb on + strand, within yajC at 426.765 kb on + strand, within yajC at 426.765 kb on + strand, within yajC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM remove 424,484 - queA b0405 0.23 -1.4 424,484 - queA b0405 0.23 -0.5 424,612 + queA b0405 0.35 -0.9 424,651 - queA b0405 0.39 -0.9 424,651 - queA b0405 0.39 -0.2 424,651 - queA b0405 0.39 -0.5 424,657 + queA b0405 0.39 -1.2 424,668 + queA b0405 0.40 -0.5 424,668 + queA b0405 0.40 +0.4 424,922 - queA b0405 0.64 -0.5 425,147 - queA b0405 0.85 -0.4 425,147 - queA b0405 0.85 -1.4 425,300 + +0.6 425,300 + +0.1 425,436 - -0.1 425,470 + -1.0 425,700 - tgt b0406 0.30 +0.1 425,700 - tgt b0406 0.30 -0.2 425,852 - tgt b0406 0.44 -1.4 425,914 + tgt b0406 0.49 -0.2 425,945 - tgt b0406 0.52 -1.5 425,945 - -0.5 425,964 + tgt b0406 0.53 -1.8 426,030 - tgt b0406 0.59 -1.5 426,030 - tgt b0406 0.59 -0.1 426,035 - tgt b0406 0.60 +0.1 426,035 - tgt b0406 0.60 -2.5 426,230 + tgt b0406 0.77 -1.8 426,230 + tgt b0406 0.77 -2.1 426,241 + tgt b0406 0.78 -1.1 426,249 - tgt b0406 0.79 -2.2 426,259 - tgt b0406 0.80 -2.2 426,277 - tgt b0406 0.81 -1.8 426,318 - tgt b0406 0.85 -3.4 426,352 - tgt b0406 0.88 -3.4 426,352 - tgt b0406 0.88 -0.8 426,352 - tgt b0406 0.88 -2.5 426,352 - tgt b0406 0.88 -2.4 426,405 + -1.2 426,481 - +1.1 426,561 + yajC b0407 0.15 -1.5 426,666 + yajC b0407 0.47 -1.7 426,666 + yajC b0407 0.47 -2.5 426,765 + yajC b0407 0.76 -2.4 426,765 + yajC b0407 0.76 -0.7
Or see this region's nucleotide sequence