Strain Fitness in Escherichia coli BW25113 around b2242

Experiment: M9 Anaerobic Capecitabine 5000 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglpA and glpB overlap by 11 nucleotidesglpB and glpC overlap by 4 nucleotides b2241: glpA - sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI), at 2,350,669 to 2,352,297 glpA b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546 glpB b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733 glpC Position (kb) 2352 2353 2354Strain fitness (log2 ratio) -2 -1 0 1at 2351.298 kb on + strand, within glpAat 2351.353 kb on + strand, within glpAat 2351.353 kb on + strand, within glpAat 2351.392 kb on - strand, within glpAat 2351.608 kb on - strand, within glpAat 2351.686 kb on - strand, within glpAat 2351.686 kb on - strand, within glpAat 2351.773 kb on + strand, within glpAat 2351.773 kb on + strand, within glpAat 2351.792 kb on + strand, within glpAat 2351.792 kb on + strand, within glpAat 2351.801 kb on + strand, within glpAat 2351.801 kb on + strand, within glpAat 2351.802 kb on + strand, within glpAat 2351.802 kb on + strand, within glpAat 2351.809 kb on - strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.826 kb on + strand, within glpAat 2351.947 kb on + strand, within glpAat 2351.947 kb on + strand, within glpAat 2351.955 kb on - strand, within glpAat 2351.955 kb on - strand, within glpAat 2352.006 kb on - strand, within glpAat 2352.059 kb on + strand, within glpAat 2352.059 kb on + strand, within glpAat 2352.067 kb on - strand, within glpAat 2352.074 kb on - strand, within glpAat 2352.104 kb on + strand, within glpAat 2352.104 kb on + strand, within glpAat 2352.122 kb on + strand, within glpAat 2352.122 kb on + strand, within glpAat 2352.265 kb on + strandat 2352.300 kb on - strandat 2352.326 kb on + strandat 2352.347 kb on + strandat 2352.369 kb on + strandat 2352.440 kb on - strand, within glpBat 2352.447 kb on + strand, within glpBat 2352.461 kb on - strand, within glpBat 2352.463 kb on + strand, within glpBat 2352.463 kb on + strand, within glpBat 2352.506 kb on + strand, within glpBat 2352.506 kb on + strand, within glpBat 2352.547 kb on + strand, within glpBat 2352.631 kb on + strand, within glpBat 2352.650 kb on + strand, within glpBat 2352.650 kb on + strand, within glpBat 2352.787 kb on - strand, within glpBat 2353.281 kb on + strand, within glpBat 2353.291 kb on + strand, within glpBat 2353.291 kb on + strand, within glpBat 2353.326 kb on - strand, within glpBat 2353.326 kb on - strand, within glpBat 2353.379 kb on - strand, within glpBat 2353.400 kb on + strand, within glpBat 2353.400 kb on + strand, within glpBat 2353.436 kb on + strandat 2353.436 kb on + strandat 2353.446 kb on + strandat 2353.575 kb on - strandat 2353.575 kb on - strandat 2353.577 kb on + strandat 2353.577 kb on + strandat 2353.615 kb on - strandat 2353.684 kb on + strand, within glpCat 2353.686 kb on + strand, within glpCat 2353.696 kb on - strand, within glpCat 2353.696 kb on - strand, within glpCat 2353.767 kb on + strand, within glpCat 2353.812 kb on + strand, within glpCat 2353.853 kb on + strand, within glpCat 2353.853 kb on + strand, within glpCat 2353.871 kb on - strand, within glpCat 2354.115 kb on - strand, within glpCat 2354.125 kb on - strand, within glpCat 2354.125 kb on - strand, within glpCat 2354.169 kb on + strand, within glpCat 2354.278 kb on - strand, within glpCat 2354.438 kb on - strand, within glpCat 2354.534 kb on + strand, within glpCat 2354.542 kb on - strand, within glpC

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic Capecitabine 5000 uM
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2,351,298 + glpA b2241 0.39 -0.8
2,351,353 + glpA b2241 0.42 -0.9
2,351,353 + glpA b2241 0.42 -1.1
2,351,392 - glpA b2241 0.44 +0.2
2,351,608 - glpA b2241 0.58 -0.2
2,351,686 - glpA b2241 0.62 -0.1
2,351,686 - glpA b2241 0.62 +0.8
2,351,773 + glpA b2241 0.68 +0.7
2,351,773 + glpA b2241 0.68 -0.1
2,351,792 + glpA b2241 0.69 -0.3
2,351,792 + glpA b2241 0.69 -0.1
2,351,801 + glpA b2241 0.69 +0.4
2,351,801 + glpA b2241 0.69 -0.2
2,351,802 + glpA b2241 0.70 -0.6
2,351,802 + glpA b2241 0.70 +0.0
2,351,809 - glpA b2241 0.70 -0.6
2,351,816 + glpA b2241 0.70 +0.2
2,351,816 + glpA b2241 0.70 -1.0
2,351,816 + glpA b2241 0.70 +0.7
2,351,816 + glpA b2241 0.70 -1.2
2,351,826 + glpA b2241 0.71 -0.9
2,351,947 + glpA b2241 0.78 +0.3
2,351,947 + glpA b2241 0.78 +0.8
2,351,955 - glpA b2241 0.79 -0.7
2,351,955 - glpA b2241 0.79 +0.3
2,352,006 - glpA b2241 0.82 +0.5
2,352,059 + glpA b2241 0.85 -0.2
2,352,059 + glpA b2241 0.85 -0.1
2,352,067 - glpA b2241 0.86 +0.3
2,352,074 - glpA b2241 0.86 +0.0
2,352,104 + glpA b2241 0.88 +0.6
2,352,104 + glpA b2241 0.88 +0.6
2,352,122 + glpA b2241 0.89 -0.7
2,352,122 + glpA b2241 0.89 -0.4
2,352,265 + +0.5
2,352,300 - -0.3
2,352,326 + -0.7
2,352,347 + +0.2
2,352,369 + -1.1
2,352,440 - glpB b2242 0.12 -0.3
2,352,447 + glpB b2242 0.13 -0.5
2,352,461 - glpB b2242 0.14 +0.3
2,352,463 + glpB b2242 0.14 -0.8
2,352,463 + glpB b2242 0.14 -0.0
2,352,506 + glpB b2242 0.17 +0.1
2,352,506 + glpB b2242 0.17 +0.8
2,352,547 + glpB b2242 0.21 +0.3
2,352,631 + glpB b2242 0.27 -0.1
2,352,650 + glpB b2242 0.29 -1.8
2,352,650 + glpB b2242 0.29 -1.5
2,352,787 - glpB b2242 0.40 +0.1
2,353,281 + glpB b2242 0.79 -0.4
2,353,291 + glpB b2242 0.80 +0.1
2,353,291 + glpB b2242 0.80 -0.0
2,353,326 - glpB b2242 0.82 -0.8
2,353,326 - glpB b2242 0.82 +0.7
2,353,379 - glpB b2242 0.87 +0.0
2,353,400 + glpB b2242 0.88 +0.4
2,353,400 + glpB b2242 0.88 -0.6
2,353,436 + -0.2
2,353,436 + -0.4
2,353,446 + -0.3
2,353,575 - +0.3
2,353,575 - -0.9
2,353,577 + -0.5
2,353,577 + -0.1
2,353,615 - -0.7
2,353,684 + glpC b2243 0.12 -0.2
2,353,686 + glpC b2243 0.12 +0.4
2,353,696 - glpC b2243 0.13 +0.7
2,353,696 - glpC b2243 0.13 -1.4
2,353,767 + glpC b2243 0.19 -0.4
2,353,812 + glpC b2243 0.23 -0.7
2,353,853 + glpC b2243 0.26 -0.9
2,353,853 + glpC b2243 0.26 -1.0
2,353,871 - glpC b2243 0.28 -0.7
2,354,115 - glpC b2243 0.48 -0.4
2,354,125 - glpC b2243 0.49 -1.3
2,354,125 - glpC b2243 0.49 +0.9
2,354,169 + glpC b2243 0.53 -0.2
2,354,278 - glpC b2243 0.62 -0.4
2,354,438 - glpC b2243 0.75 -0.7
2,354,534 + glpC b2243 0.83 -0.9
2,354,542 - glpC b2243 0.84 +0.2

Or see this region's nucleotide sequence