Strain Fitness in Escherichia coli BW25113 around b2411

Experiment: M9 Anaerobic Capecitabine 5000 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeH and ypeB overlap by 4 nucleotidesypeB and ligA are separated by 1 nucleotidesligA and zipA are separated by 70 nucleotides b2410: yfeH - predicted inner membrane protein (NCBI), at 2,524,968 to 2,525,966 yfeH b4546: ypeB - hypothetical protein (NCBI), at 2,525,963 to 2,526,181 ypeB b2411: ligA - NAD-dependent DNA ligase LigA (NCBI), at 2,526,183 to 2,528,198 ligA b2412: zipA - cell division protein ZipA (NCBI), at 2,528,269 to 2,529,255 zipA Position (kb) 2526 2527 2528 2529Strain fitness (log2 ratio) -2 -1 0 1at 2525.248 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.613 kb on + strand, within yfeHat 2525.709 kb on + strand, within yfeHat 2525.727 kb on - strand, within yfeHat 2525.727 kb on - strand, within yfeHat 2525.887 kb on + strandat 2525.942 kb on + strandat 2526.092 kb on + strand, within ypeBat 2528.200 kb on - strandat 2528.200 kb on - strandat 2528.257 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Anaerobic Capecitabine 5000 uM
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2,525,248 - yfeH b2410 0.28 +0.4
2,525,454 - yfeH b2410 0.49 +0.7
2,525,454 - yfeH b2410 0.49 +0.4
2,525,454 - yfeH b2410 0.49 -0.1
2,525,454 - yfeH b2410 0.49 -0.2
2,525,613 + yfeH b2410 0.65 -0.7
2,525,709 + yfeH b2410 0.74 -0.6
2,525,727 - yfeH b2410 0.76 -1.9
2,525,727 - yfeH b2410 0.76 +0.5
2,525,887 + -0.8
2,525,942 + -0.5
2,526,092 + ypeB b4546 0.59 -0.5
2,528,200 - -0.0
2,528,200 - -0.4
2,528,257 + +1.0

Or see this region's nucleotide sequence