Experiment: M9 Anaerobic Capecitabine 5000 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbS and allA are separated by 229 nucleotides allA and allR are separated by 77 nucleotides allR and gcl are separated by 89 nucleotides
b0504: ybbS - DNA-binding transcriptional activator of the allD operon (NCBI), at 530,519 to 531,445
ybbS
b0505: allA - ureidoglycolate hydrolase (NCBI), at 531,675 to 532,157
allA
b0506: allR - DNA-binding transcriptional repressor (NCBI), at 532,235 to 533,050
allR
b0507: gcl - glyoxylate carboligase (NCBI), at 533,140 to 534,921
gcl
Position (kb) 531
532
533 Strain fitness (log2 ratio) -1
0
1 at 531.058 kb on - strand, within ybbS at 531.077 kb on - strand, within ybbS at 531.113 kb on + strand, within ybbS at 531.134 kb on - strand, within ybbS at 531.229 kb on + strand, within ybbS at 531.687 kb on + strand at 531.687 kb on + strand at 531.776 kb on + strand, within allA at 531.816 kb on + strand, within allA at 531.907 kb on - strand, within allA at 531.962 kb on - strand, within allA at 532.199 kb on + strand at 532.216 kb on - strand at 532.221 kb on + strand at 532.278 kb on + strand at 532.278 kb on + strand at 532.497 kb on - strand, within allR at 532.513 kb on - strand, within allR at 532.560 kb on + strand, within allR at 532.616 kb on + strand, within allR at 532.642 kb on - strand, within allR at 532.657 kb on - strand, within allR at 532.677 kb on + strand, within allR at 532.677 kb on + strand, within allR at 532.798 kb on - strand, within allR at 532.798 kb on - strand, within allR at 532.811 kb on + strand, within allR at 532.811 kb on + strand, within allR at 532.840 kb on + strand, within allR at 532.840 kb on + strand, within allR at 532.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Anaerobic Capecitabine 5000 uM remove 531,058 - ybbS b0504 0.58 -1.1 531,077 - ybbS b0504 0.60 +0.1 531,113 + ybbS b0504 0.64 -0.5 531,134 - ybbS b0504 0.66 -1.5 531,229 + ybbS b0504 0.77 +0.1 531,687 + -1.4 531,687 + -1.2 531,776 + allA b0505 0.21 -0.2 531,816 + allA b0505 0.29 -1.0 531,907 - allA b0505 0.48 +0.3 531,962 - allA b0505 0.59 -0.9 532,199 + -0.1 532,216 - +0.1 532,221 + -0.1 532,278 + +1.2 532,278 + -0.3 532,497 - allR b0506 0.32 -0.3 532,513 - allR b0506 0.34 -1.4 532,560 + allR b0506 0.40 +0.4 532,616 + allR b0506 0.47 +0.6 532,642 - allR b0506 0.50 +0.0 532,657 - allR b0506 0.52 +0.9 532,677 + allR b0506 0.54 +0.8 532,677 + allR b0506 0.54 +0.1 532,798 - allR b0506 0.69 +0.9 532,798 - allR b0506 0.69 -0.7 532,811 + allR b0506 0.71 +0.1 532,811 + allR b0506 0.71 +0.9 532,840 + allR b0506 0.74 +0.8 532,840 + allR b0506 0.74 +0.1 532,992 - +0.5
Or see this region's nucleotide sequence