Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdpA and kdpF overlap by 1 nucleotides kdpF and ybfA are separated by 312 nucleotides ybfA and rhsC are separated by 242 nucleotides
b0698: kdpA - potassium-transporting ATPase subunit A (NCBI), at 726,282 to 727,955
kdpA
b4513: kdpF - potassium ion accessory transporter subunit (NCBI), at 727,955 to 728,044
kdpF
b0699: ybfA - hypothetical protein (NCBI), at 728,357 to 728,563
ybfA
b0700: rhsC - rhsC element core protein RshC (NCBI), at 728,806 to 732,999
rhsC
Position (kb) 727
728
729 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 726.981 kb on + strand, within kdpA at 727.062 kb on - strand, within kdpA at 727.075 kb on - strand, within kdpA at 727.075 kb on - strand, within kdpA at 727.107 kb on - strand, within kdpA at 727.325 kb on - strand, within kdpA at 727.338 kb on + strand, within kdpA at 727.338 kb on + strand, within kdpA at 727.435 kb on - strand, within kdpA at 727.464 kb on + strand, within kdpA at 727.600 kb on + strand, within kdpA at 727.600 kb on + strand, within kdpA at 727.640 kb on - strand, within kdpA at 727.687 kb on - strand, within kdpA at 727.692 kb on - strand, within kdpA at 727.696 kb on + strand, within kdpA at 727.696 kb on + strand, within kdpA at 727.696 kb on + strand, within kdpA at 727.767 kb on + strand, within kdpA at 727.792 kb on + strand at 727.871 kb on + strand at 727.871 kb on + strand at 727.884 kb on + strand at 727.904 kb on - strand at 727.904 kb on - strand at 727.963 kb on + strand at 727.966 kb on - strand at 727.988 kb on - strand, within kdpF at 728.022 kb on - strand, within kdpF at 728.054 kb on - strand at 728.054 kb on - strand at 728.059 kb on - strand at 728.068 kb on - strand at 728.076 kb on - strand at 728.580 kb on + strand at 728.597 kb on + strand at 728.641 kb on + strand at 728.641 kb on + strand at 728.758 kb on - strand at 728.810 kb on - strand at 728.840 kb on - strand at 728.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 726,981 + kdpA b0698 0.42 -1.0 727,062 - kdpA b0698 0.47 -0.4 727,075 - kdpA b0698 0.47 +1.8 727,075 - kdpA b0698 0.47 -1.1 727,107 - kdpA b0698 0.49 -0.5 727,325 - kdpA b0698 0.62 -2.6 727,338 + kdpA b0698 0.63 -0.0 727,338 + kdpA b0698 0.63 -3.8 727,435 - kdpA b0698 0.69 -1.0 727,464 + kdpA b0698 0.71 -0.9 727,600 + kdpA b0698 0.79 -1.7 727,600 + kdpA b0698 0.79 -0.3 727,640 - kdpA b0698 0.81 -0.3 727,687 - kdpA b0698 0.84 +0.4 727,692 - kdpA b0698 0.84 -2.1 727,696 + kdpA b0698 0.84 -1.3 727,696 + kdpA b0698 0.84 -1.0 727,696 + kdpA b0698 0.84 -0.8 727,767 + kdpA b0698 0.89 -0.7 727,792 + -1.4 727,871 + +1.3 727,871 + -2.5 727,884 + +0.9 727,904 - -0.7 727,904 - -0.2 727,963 + -0.4 727,966 - -0.4 727,988 - kdpF b4513 0.37 -3.2 728,022 - kdpF b4513 0.74 -0.9 728,054 - +0.2 728,054 - -1.0 728,059 - -2.6 728,068 - +2.4 728,076 - -1.0 728,580 + +0.1 728,597 + -0.7 728,641 + -0.3 728,641 + -0.9 728,758 - -0.3 728,810 - -0.2 728,840 - -0.1 728,863 - -0.2
Or see this region's nucleotide sequence