Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nepI and yicN are separated by 210 nucleotides yicN and yicO are separated by 52 nucleotides
b3662: nepI - predicted transporter (RefSeq), at 3,838,572 to 3,839,762
nepI
b3663: yicN - orf, hypothetical protein (VIMSS), at 3,839,973 to 3,840,425
yicN
b3664: yicO - orf, hypothetical protein (VIMSS), at 3,840,478 to 3,841,812
yicO
Position (kb)
3839
3840
3841 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3838.973 kb on + strand, within nepI at 3839.005 kb on - strand, within nepI at 3839.007 kb on + strand, within nepI at 3839.031 kb on + strand, within nepI at 3839.039 kb on - strand, within nepI at 3839.039 kb on - strand, within nepI at 3839.115 kb on - strand, within nepI at 3839.115 kb on - strand, within nepI at 3839.145 kb on - strand, within nepI at 3839.209 kb on + strand, within nepI at 3839.209 kb on + strand, within nepI at 3839.284 kb on + strand, within nepI at 3839.284 kb on + strand, within nepI at 3839.284 kb on + strand, within nepI at 3839.367 kb on - strand, within nepI at 3839.372 kb on - strand, within nepI at 3839.372 kb on - strand, within nepI at 3839.405 kb on + strand, within nepI at 3839.405 kb on + strand, within nepI at 3839.511 kb on - strand, within nepI at 3839.511 kb on - strand, within nepI at 3839.772 kb on - strand at 3839.878 kb on + strand at 3839.955 kb on - strand at 3839.955 kb on - strand at 3839.960 kb on - strand at 3839.974 kb on + strand at 3840.021 kb on - strand, within yicN at 3840.085 kb on + strand, within yicN at 3840.085 kb on + strand, within yicN at 3840.200 kb on - strand, within yicN at 3840.200 kb on - strand, within yicN at 3840.204 kb on - strand, within yicN at 3840.204 kb on - strand, within yicN at 3840.289 kb on + strand, within yicN at 3840.297 kb on - strand, within yicN at 3840.304 kb on + strand, within yicN at 3840.304 kb on + strand, within yicN at 3840.316 kb on + strand, within yicN at 3840.316 kb on + strand, within yicN at 3840.324 kb on - strand, within yicN at 3840.357 kb on - strand, within yicN at 3840.371 kb on + strand, within yicN at 3840.420 kb on + strand at 3840.420 kb on + strand at 3840.431 kb on + strand at 3840.501 kb on + strand at 3840.525 kb on - strand at 3840.542 kb on - strand at 3840.585 kb on - strand at 3840.602 kb on - strand at 3840.602 kb on - strand at 3840.626 kb on + strand, within yicO at 3840.626 kb on + strand, within yicO at 3840.636 kb on + strand, within yicO at 3840.636 kb on + strand, within yicO at 3840.657 kb on - strand, within yicO at 3840.696 kb on - strand, within yicO at 3840.729 kb on + strand, within yicO at 3840.773 kb on - strand, within yicO at 3840.792 kb on + strand, within yicO at 3840.792 kb on + strand, within yicO at 3840.804 kb on + strand, within yicO at 3840.814 kb on + strand, within yicO at 3840.814 kb on + strand, within yicO at 3840.814 kb on + strand, within yicO at 3840.822 kb on - strand, within yicO at 3840.822 kb on - strand, within yicO at 3840.827 kb on - strand, within yicO at 3840.827 kb on - strand, within yicO at 3840.827 kb on - strand at 3840.839 kb on + strand, within yicO at 3840.839 kb on + strand, within yicO at 3840.839 kb on + strand, within yicO at 3840.848 kb on - strand, within yicO at 3840.851 kb on - strand, within yicO at 3840.855 kb on + strand, within yicO at 3840.872 kb on + strand, within yicO at 3840.873 kb on - strand, within yicO at 3840.873 kb on - strand, within yicO at 3840.873 kb on - strand, within yicO at 3840.873 kb on - strand, within yicO at 3840.878 kb on - strand, within yicO at 3840.905 kb on + strand, within yicO at 3840.905 kb on + strand, within yicO at 3840.905 kb on + strand, within yicO at 3840.906 kb on + strand, within yicO at 3840.910 kb on + strand, within yicO at 3840.913 kb on - strand, within yicO at 3840.913 kb on - strand, within yicO at 3840.916 kb on + strand, within yicO at 3840.916 kb on + strand, within yicO at 3840.916 kb on + strand, within yicO at 3840.916 kb on + strand, within yicO at 3840.920 kb on + strand, within yicO at 3840.920 kb on + strand, within yicO at 3840.925 kb on - strand, within yicO at 3840.925 kb on - strand, within yicO at 3840.925 kb on - strand, within yicO at 3840.925 kb on - strand, within yicO at 3840.928 kb on - strand, within yicO at 3840.928 kb on - strand, within yicO at 3840.928 kb on - strand, within yicO at 3840.929 kb on + strand, within yicO at 3840.941 kb on - strand, within yicO at 3840.958 kb on + strand, within yicO at 3840.960 kb on + strand, within yicO at 3840.960 kb on + strand, within yicO at 3840.962 kb on + strand, within yicO at 3840.962 kb on + strand, within yicO at 3840.964 kb on + strand, within yicO at 3840.966 kb on - strand, within yicO at 3840.968 kb on - strand, within yicO at 3840.971 kb on - strand, within yicO at 3840.981 kb on + strand, within yicO at 3840.981 kb on + strand, within yicO at 3840.981 kb on + strand, within yicO at 3840.991 kb on + strand, within yicO at 3840.998 kb on + strand, within yicO at 3840.998 kb on + strand, within yicO at 3841.007 kb on - strand, within yicO at 3841.026 kb on + strand, within yicO at 3841.026 kb on + strand, within yicO at 3841.053 kb on + strand, within yicO at 3841.059 kb on - strand, within yicO at 3841.061 kb on - strand, within yicO at 3841.077 kb on + strand, within yicO at 3841.077 kb on + strand, within yicO at 3841.080 kb on - strand, within yicO at 3841.087 kb on + strand, within yicO at 3841.118 kb on + strand, within yicO at 3841.121 kb on - strand, within yicO at 3841.127 kb on + strand at 3841.127 kb on - strand, within yicO at 3841.145 kb on + strand, within yicO at 3841.176 kb on + strand, within yicO at 3841.176 kb on + strand, within yicO at 3841.176 kb on + strand, within yicO at 3841.176 kb on + strand, within yicO at 3841.200 kb on + strand, within yicO at 3841.214 kb on + strand, within yicO at 3841.214 kb on + strand, within yicO at 3841.265 kb on + strand, within yicO at 3841.265 kb on + strand, within yicO at 3841.273 kb on + strand, within yicO at 3841.273 kb on + strand, within yicO at 3841.274 kb on - strand, within yicO at 3841.282 kb on + strand, within yicO at 3841.292 kb on - strand, within yicO at 3841.292 kb on - strand, within yicO at 3841.298 kb on - strand, within yicO at 3841.302 kb on - strand, within yicO at 3841.363 kb on - strand, within yicO at 3841.366 kb on - strand, within yicO at 3841.366 kb on - strand, within yicO at 3841.371 kb on - strand, within yicO at 3841.371 kb on - strand, within yicO at 3841.371 kb on - strand, within yicO at 3841.371 kb on - strand, within yicO at 3841.371 kb on - strand, within yicO at 3841.376 kb on - strand, within yicO at 3841.378 kb on - strand, within yicO at 3841.378 kb on - strand, within yicO at 3841.381 kb on + strand, within yicO at 3841.383 kb on - strand, within yicO at 3841.383 kb on - strand, within yicO at 3841.411 kb on - strand, within yicO at 3841.411 kb on - strand, within yicO at 3841.411 kb on - strand, within yicO at 3841.414 kb on - strand, within yicO at 3841.415 kb on + strand, within yicO at 3841.416 kb on - strand, within yicO at 3841.416 kb on - strand, within yicO at 3841.416 kb on - strand, within yicO at 3841.421 kb on - strand, within yicO at 3841.421 kb on - strand, within yicO at 3841.423 kb on - strand, within yicO at 3841.423 kb on - strand, within yicO
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 3,838,973 + nepI b3662 0.34 -0.6 3,839,005 - nepI b3662 0.36 -0.6 3,839,007 + nepI b3662 0.37 -1.2 3,839,031 + nepI b3662 0.39 +0.0 3,839,039 - nepI b3662 0.39 -1.0 3,839,039 - nepI b3662 0.39 -1.0 3,839,115 - nepI b3662 0.46 -0.1 3,839,115 - nepI b3662 0.46 -2.0 3,839,145 - nepI b3662 0.48 +1.5 3,839,209 + nepI b3662 0.53 +0.9 3,839,209 + nepI b3662 0.53 -1.6 3,839,284 + nepI b3662 0.60 -0.9 3,839,284 + nepI b3662 0.60 -1.1 3,839,284 + nepI b3662 0.60 -1.5 3,839,367 - nepI b3662 0.67 -1.5 3,839,372 - nepI b3662 0.67 +0.5 3,839,372 - nepI b3662 0.67 -0.4 3,839,405 + nepI b3662 0.70 -0.3 3,839,405 + nepI b3662 0.70 -0.4 3,839,511 - nepI b3662 0.79 -0.4 3,839,511 - nepI b3662 0.79 +1.1 3,839,772 - -0.5 3,839,878 + +0.3 3,839,955 - +1.5 3,839,955 - +0.0 3,839,960 - +1.0 3,839,974 + -0.6 3,840,021 - yicN b3663 0.11 -1.3 3,840,085 + yicN b3663 0.25 -3.2 3,840,085 + yicN b3663 0.25 -1.6 3,840,200 - yicN b3663 0.50 -0.6 3,840,200 - yicN b3663 0.50 -1.0 3,840,204 - yicN b3663 0.51 -2.3 3,840,204 - yicN b3663 0.51 +0.2 3,840,289 + yicN b3663 0.70 -0.5 3,840,297 - yicN b3663 0.72 -0.4 3,840,304 + yicN b3663 0.73 +0.2 3,840,304 + yicN b3663 0.73 -2.0 3,840,316 + yicN b3663 0.76 +0.1 3,840,316 + yicN b3663 0.76 -3.0 3,840,324 - yicN b3663 0.77 -1.9 3,840,357 - yicN b3663 0.85 -2.5 3,840,371 + yicN b3663 0.88 -1.5 3,840,420 + -0.9 3,840,420 + +0.0 3,840,431 + -1.0 3,840,501 + -0.7 3,840,525 - +0.5 3,840,542 - -0.1 3,840,585 - -0.1 3,840,602 - -0.9 3,840,602 - +0.4 3,840,626 + yicO b3664 0.11 -0.9 3,840,626 + yicO b3664 0.11 -2.4 3,840,636 + yicO b3664 0.12 -0.6 3,840,636 + yicO b3664 0.12 -1.2 3,840,657 - yicO b3664 0.13 -0.2 3,840,696 - yicO b3664 0.16 -1.4 3,840,729 + yicO b3664 0.19 +0.1 3,840,773 - yicO b3664 0.22 +0.1 3,840,792 + yicO b3664 0.24 -0.1 3,840,792 + yicO b3664 0.24 -0.7 3,840,804 + yicO b3664 0.24 -0.5 3,840,814 + yicO b3664 0.25 -0.0 3,840,814 + yicO b3664 0.25 -1.0 3,840,814 + yicO b3664 0.25 -0.5 3,840,822 - yicO b3664 0.26 -3.0 3,840,822 - yicO b3664 0.26 -1.4 3,840,827 - yicO b3664 0.26 -1.5 3,840,827 - yicO b3664 0.26 -0.4 3,840,827 - -1.9 3,840,839 + yicO b3664 0.27 -1.5 3,840,839 + yicO b3664 0.27 -3.3 3,840,839 + yicO b3664 0.27 -3.6 3,840,848 - yicO b3664 0.28 -0.9 3,840,851 - yicO b3664 0.28 -1.9 3,840,855 + yicO b3664 0.28 +0.2 3,840,872 + yicO b3664 0.30 -0.4 3,840,873 - yicO b3664 0.30 -0.7 3,840,873 - yicO b3664 0.30 -0.8 3,840,873 - yicO b3664 0.30 -0.4 3,840,873 - yicO b3664 0.30 -1.7 3,840,878 - yicO b3664 0.30 -0.1 3,840,905 + yicO b3664 0.32 -0.5 3,840,905 + yicO b3664 0.32 +1.0 3,840,905 + yicO b3664 0.32 -0.3 3,840,906 + yicO b3664 0.32 +0.4 3,840,910 + yicO b3664 0.32 -1.5 3,840,913 - yicO b3664 0.33 -2.7 3,840,913 - yicO b3664 0.33 +0.5 3,840,916 + yicO b3664 0.33 -0.8 3,840,916 + yicO b3664 0.33 -1.5 3,840,916 + yicO b3664 0.33 -0.7 3,840,916 + yicO b3664 0.33 -2.3 3,840,920 + yicO b3664 0.33 +0.2 3,840,920 + yicO b3664 0.33 +0.0 3,840,925 - yicO b3664 0.33 -1.4 3,840,925 - yicO b3664 0.33 -0.5 3,840,925 - yicO b3664 0.33 +0.8 3,840,925 - yicO b3664 0.33 +0.5 3,840,928 - yicO b3664 0.34 +0.2 3,840,928 - yicO b3664 0.34 -1.7 3,840,928 - yicO b3664 0.34 -0.3 3,840,929 + yicO b3664 0.34 +0.4 3,840,941 - yicO b3664 0.35 -2.1 3,840,958 + yicO b3664 0.36 -0.4 3,840,960 + yicO b3664 0.36 -0.5 3,840,960 + yicO b3664 0.36 +0.1 3,840,962 + yicO b3664 0.36 -1.7 3,840,962 + yicO b3664 0.36 -3.4 3,840,964 + yicO b3664 0.36 -0.9 3,840,966 - yicO b3664 0.37 -2.2 3,840,968 - yicO b3664 0.37 -1.6 3,840,971 - yicO b3664 0.37 -2.5 3,840,981 + yicO b3664 0.38 -2.6 3,840,981 + yicO b3664 0.38 -1.4 3,840,981 + yicO b3664 0.38 -0.6 3,840,991 + yicO b3664 0.38 -2.4 3,840,998 + yicO b3664 0.39 +0.5 3,840,998 + yicO b3664 0.39 +1.1 3,841,007 - yicO b3664 0.40 +2.3 3,841,026 + yicO b3664 0.41 +1.0 3,841,026 + yicO b3664 0.41 +0.2 3,841,053 + yicO b3664 0.43 -1.5 3,841,059 - yicO b3664 0.44 +0.8 3,841,061 - yicO b3664 0.44 +2.8 3,841,077 + yicO b3664 0.45 -0.1 3,841,077 + yicO b3664 0.45 +2.8 3,841,080 - yicO b3664 0.45 +0.9 3,841,087 + yicO b3664 0.46 -0.4 3,841,118 + yicO b3664 0.48 -0.7 3,841,121 - yicO b3664 0.48 +1.9 3,841,127 + +2.4 3,841,127 - yicO b3664 0.49 +2.3 3,841,145 + yicO b3664 0.50 +0.3 3,841,176 + yicO b3664 0.52 +1.2 3,841,176 + yicO b3664 0.52 -0.0 3,841,176 + yicO b3664 0.52 -0.7 3,841,176 + yicO b3664 0.52 +0.3 3,841,200 + yicO b3664 0.54 +0.0 3,841,214 + yicO b3664 0.55 -0.7 3,841,214 + yicO b3664 0.55 +1.9 3,841,265 + yicO b3664 0.59 +0.2 3,841,265 + yicO b3664 0.59 -1.3 3,841,273 + yicO b3664 0.60 -1.6 3,841,273 + yicO b3664 0.60 -1.4 3,841,274 - yicO b3664 0.60 -1.4 3,841,282 + yicO b3664 0.60 -1.0 3,841,292 - yicO b3664 0.61 -1.0 3,841,292 - yicO b3664 0.61 -1.9 3,841,298 - yicO b3664 0.61 -1.1 3,841,302 - yicO b3664 0.62 +2.5 3,841,363 - yicO b3664 0.66 -1.9 3,841,366 - yicO b3664 0.67 -0.5 3,841,366 - yicO b3664 0.67 -0.2 3,841,371 - yicO b3664 0.67 -2.1 3,841,371 - yicO b3664 0.67 -0.3 3,841,371 - yicO b3664 0.67 +0.2 3,841,371 - yicO b3664 0.67 -0.4 3,841,371 - yicO b3664 0.67 +0.5 3,841,376 - yicO b3664 0.67 -1.2 3,841,378 - yicO b3664 0.67 -2.3 3,841,378 - yicO b3664 0.67 +1.1 3,841,381 + yicO b3664 0.68 +2.3 3,841,383 - yicO b3664 0.68 -0.0 3,841,383 - yicO b3664 0.68 -1.4 3,841,411 - yicO b3664 0.70 +1.2 3,841,411 - yicO b3664 0.70 +0.1 3,841,411 - yicO b3664 0.70 -1.7 3,841,414 - yicO b3664 0.70 -0.9 3,841,415 + yicO b3664 0.70 -0.9 3,841,416 - yicO b3664 0.70 +0.2 3,841,416 - yicO b3664 0.70 -1.0 3,841,416 - yicO b3664 0.70 -0.9 3,841,421 - yicO b3664 0.71 -1.3 3,841,421 - yicO b3664 0.71 -0.6 3,841,423 - yicO b3664 0.71 +0.5 3,841,423 - yicO b3664 0.71 -1.4
Or see this region's nucleotide sequence