Strain Fitness in Escherichia coli BW25113 around b1891

Experiment: mariner vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmotA and flhC are separated by 126 nucleotidesflhC and flhD are separated by 2 nucleotidesflhD and insB-5 are separated by 320 nucleotides b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163 motA b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868 flhC b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221 flhD b1893: insB-5 - IS1 transposase InsAB' (NCBI), at 1,976,542 to 1,977,045 insB-5 Position (kb) 1975 1976Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1974.414 kb on + strand, within motAat 1974.854 kb on - strand, within motAat 1974.934 kb on + strand, within motAat 1974.934 kb on + strand, within motAat 1975.040 kb on + strand, within motAat 1975.092 kb on + strandat 1975.093 kb on - strandat 1975.173 kb on + strandat 1975.179 kb on + strandat 1975.179 kb on + strandat 1975.415 kb on - strand, within flhCat 1975.477 kb on + strand, within flhCat 1975.589 kb on + strand, within flhCat 1975.636 kb on + strand, within flhCat 1975.707 kb on + strand, within flhCat 1975.914 kb on - strand, within flhDat 1975.961 kb on - strand, within flhDat 1975.961 kb on - strand, within flhDat 1975.991 kb on + strand, within flhDat 1975.991 kb on - strand, within flhDat 1976.072 kb on + strand, within flhDat 1976.072 kb on + strand, within flhDat 1976.072 kb on + strand, within flhDat 1976.120 kb on + strand, within flhDat 1976.120 kb on + strand, within flhDat 1976.138 kb on + strand, within flhDat 1976.146 kb on - strand, within flhDat 1976.149 kb on - strand, within flhDat 1976.149 kb on - strand, within flhDat 1976.221 kb on - strandat 1976.226 kb on - strandat 1976.226 kb on - strandat 1976.259 kb on + strandat 1976.262 kb on + strandat 1976.272 kb on + strandat 1976.281 kb on + strandat 1976.354 kb on - strandat 1976.387 kb on + strandat 1976.396 kb on - strandat 1976.432 kb on - strandat 1976.436 kb on + strandat 1976.439 kb on + strandat 1976.439 kb on + strandat 1976.439 kb on + strandat 1976.439 kb on + strandat 1976.444 kb on + strandat 1976.447 kb on - strandat 1976.447 kb on - strandat 1976.447 kb on - strandat 1976.447 kb on - strandat 1976.448 kb on + strandat 1976.452 kb on - strandat 1976.452 kb on - strandat 1976.453 kb on + strandat 1976.454 kb on - strandat 1976.455 kb on - strandat 1976.460 kb on - strandat 1976.514 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay
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1,974,414 + motA b1890 0.16 +0.4
1,974,854 - motA b1890 0.65 -2.5
1,974,934 + motA b1890 0.74 +0.6
1,974,934 + motA b1890 0.74 +0.8
1,975,040 + motA b1890 0.86 -0.9
1,975,092 + +0.2
1,975,093 - -0.9
1,975,173 + +0.7
1,975,179 + -0.7
1,975,179 + +2.4
1,975,415 - flhC b1891 0.22 +1.5
1,975,477 + flhC b1891 0.32 -0.4
1,975,589 + flhC b1891 0.52 +1.7
1,975,636 + flhC b1891 0.60 +0.3
1,975,707 + flhC b1891 0.72 +1.0
1,975,914 - flhD b1892 0.12 +1.7
1,975,961 - flhD b1892 0.26 +0.9
1,975,961 - flhD b1892 0.26 -0.6
1,975,991 + flhD b1892 0.34 +1.8
1,975,991 - flhD b1892 0.34 +0.3
1,976,072 + flhD b1892 0.57 +3.2
1,976,072 + flhD b1892 0.57 +0.6
1,976,072 + flhD b1892 0.57 +0.1
1,976,120 + flhD b1892 0.71 -0.9
1,976,120 + flhD b1892 0.71 +0.3
1,976,138 + flhD b1892 0.76 +0.3
1,976,146 - flhD b1892 0.78 +3.6
1,976,149 - flhD b1892 0.79 -0.8
1,976,149 - flhD b1892 0.79 -2.0
1,976,221 - +0.4
1,976,226 - +0.7
1,976,226 - -1.5
1,976,259 + +0.6
1,976,262 + -1.6
1,976,272 + -0.9
1,976,281 + +0.8
1,976,354 - +1.2
1,976,387 + +0.6
1,976,396 - +1.2
1,976,432 - +2.8
1,976,436 + -0.3
1,976,439 + +0.9
1,976,439 + +0.1
1,976,439 + +0.6
1,976,439 + +0.3
1,976,444 + +2.8
1,976,447 - -0.0
1,976,447 - -0.5
1,976,447 - -1.7
1,976,447 - -0.2
1,976,448 + +2.1
1,976,452 - -3.1
1,976,452 - -1.1
1,976,453 + +1.5
1,976,454 - +0.8
1,976,455 - -0.2
1,976,460 - +2.4
1,976,514 - +1.2

Or see this region's nucleotide sequence