Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjN and ycjO are separated by 20 nucleotides ycjO and ycjP overlap by 14 nucleotides ycjP and ycjQ are separated by 30 nucleotides
b1310: ycjN - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 1,369,933 to 1,371,225
ycjN
b1311: ycjO - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 1,371,246 to 1,372,127
ycjO
b1312: ycjP - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 1,372,114 to 1,372,956
ycjP
b1313: ycjQ - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,372,987 to 1,374,039
ycjQ
Position (kb)
1371
1372
1373 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1370.269 kb on + strand, within ycjN at 1370.502 kb on + strand, within ycjN at 1370.527 kb on + strand, within ycjN at 1370.739 kb on - strand, within ycjN at 1370.740 kb on - strand, within ycjN at 1370.811 kb on + strand, within ycjN at 1370.899 kb on - strand, within ycjN at 1370.899 kb on - strand, within ycjN at 1370.985 kb on - strand, within ycjN at 1371.017 kb on + strand, within ycjN at 1371.153 kb on + strand at 1371.153 kb on + strand at 1371.161 kb on - strand at 1371.285 kb on + strand at 1371.323 kb on - strand at 1371.474 kb on + strand, within ycjO at 1371.474 kb on + strand, within ycjO at 1371.508 kb on - strand, within ycjO at 1371.587 kb on + strand, within ycjO at 1371.597 kb on + strand, within ycjO at 1371.652 kb on - strand, within ycjO at 1371.665 kb on - strand, within ycjO at 1371.738 kb on - strand, within ycjO at 1371.738 kb on - strand, within ycjO at 1371.778 kb on - strand, within ycjO at 1371.778 kb on - strand, within ycjO at 1371.938 kb on - strand, within ycjO at 1371.991 kb on + strand, within ycjO at 1372.224 kb on - strand, within ycjP at 1372.462 kb on + strand, within ycjP at 1372.470 kb on - strand, within ycjP at 1372.470 kb on - strand, within ycjP at 1372.546 kb on + strand, within ycjP at 1372.564 kb on - strand, within ycjP at 1372.564 kb on - strand, within ycjP at 1372.661 kb on - strand, within ycjP at 1372.673 kb on - strand, within ycjP at 1372.673 kb on - strand, within ycjP at 1372.684 kb on + strand, within ycjP at 1372.850 kb on + strand, within ycjP at 1373.016 kb on - strand at 1373.021 kb on - strand at 1373.031 kb on - strand at 1373.058 kb on - strand at 1373.110 kb on - strand, within ycjQ
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 1,370,269 + ycjN b1310 0.26 +0.2 1,370,502 + ycjN b1310 0.44 +1.6 1,370,527 + ycjN b1310 0.46 -0.4 1,370,739 - ycjN b1310 0.62 -1.5 1,370,740 - ycjN b1310 0.62 +0.5 1,370,811 + ycjN b1310 0.68 -0.8 1,370,899 - ycjN b1310 0.75 +0.1 1,370,899 - ycjN b1310 0.75 +1.7 1,370,985 - ycjN b1310 0.81 +0.0 1,371,017 + ycjN b1310 0.84 +0.4 1,371,153 + -2.6 1,371,153 + -2.9 1,371,161 - -2.7 1,371,285 + -1.1 1,371,323 - -1.6 1,371,474 + ycjO b1311 0.26 -0.6 1,371,474 + ycjO b1311 0.26 -1.2 1,371,508 - ycjO b1311 0.30 -0.8 1,371,587 + ycjO b1311 0.39 -2.6 1,371,597 + ycjO b1311 0.40 -1.6 1,371,652 - ycjO b1311 0.46 +0.3 1,371,665 - ycjO b1311 0.48 +1.6 1,371,738 - ycjO b1311 0.56 +1.9 1,371,738 - ycjO b1311 0.56 +1.0 1,371,778 - ycjO b1311 0.60 +2.1 1,371,778 - ycjO b1311 0.60 -0.1 1,371,938 - ycjO b1311 0.78 +0.1 1,371,991 + ycjO b1311 0.84 -0.1 1,372,224 - ycjP b1312 0.13 +0.9 1,372,462 + ycjP b1312 0.41 -0.3 1,372,470 - ycjP b1312 0.42 -2.0 1,372,470 - ycjP b1312 0.42 +0.1 1,372,546 + ycjP b1312 0.51 -0.3 1,372,564 - ycjP b1312 0.53 +1.1 1,372,564 - ycjP b1312 0.53 +0.6 1,372,661 - ycjP b1312 0.65 +1.1 1,372,673 - ycjP b1312 0.66 +1.5 1,372,673 - ycjP b1312 0.66 -1.1 1,372,684 + ycjP b1312 0.68 +0.8 1,372,850 + ycjP b1312 0.87 +1.3 1,373,016 - +0.7 1,373,021 - +0.1 1,373,031 - -4.0 1,373,058 - -0.9 1,373,110 - ycjQ b1313 0.12 -0.5
Or see this region's nucleotide sequence